System Introduction Applied Biosystems 7300 &7500 Real ... - CRN2M

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Component View : Matrix CalculationFrom spectra, using Background and Pure Spectra files,a matrix calculation is done giving the contributions of thedifferent dyes8¢¡¤£ £ ¥§¦©¨ ¨ Amplification Plot ViewReporter SignalSemilog or linear view9¢¡¤£ £ ¥§¦©¨ ¨
Allelic Discrimination View Single Tube, Dual Probe Detection Represents a plate read after a PCR reactionFluorescence Intensity Dye I10Fluorescence Intensity Dye II¢¡¤£ £ ¥§¦©¨ ¨ Data Collection• Step for data collectioncan be selected• Annealing/extension steprecommended• Keep extension step≥ 32 seconds11¢¡¤£ £ ¥§¦©¨ ¨
Page 1 and 2: System IntroductionApplied Biosyste
Page 3: Spectral Overlap500 nm 660 nm6¢¡
Page 7 and 8: Latest Software VersionSDS v1.4 sof
Page 10 and 11: Disposables96-well platesOptical 96
Page 12 and 13: „Help“ Function in SDS Software
Page 14 and 15: Monthly MaintenanceIf the pixel int
Page 16 and 17: After a Lamp Change• Run ROI cali
Page 18: Bi-annual Maintenance• 1) Generat

System Introduction Applied Biosystems 7300 &7500 Real ... - CRN2M

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