System Introduction Applied Biosystems 7300 &7500 Real ... - CRN2M

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7300 & 7500 hardwareExcitation source : Halogen- Tungsten lamp 7300 :– 1 excitation filter & 4 emission filters 7500 :– 5 excitation filters & 5 emission filters (matched to Ex) The emission is measured by a CCD camera7500 and 7500 Fast differ by block and electronics3¢¡¤£ £ ¥§¦©¨ ¨ Multicolor Detection 7500Dyes:SYBR ® Green 1FAMVIC ®JOENEDTAMRA, Cy3 ROX, TEXAS RED ®Cy5 528 nm530 nm554 nm554 nm576 nm582 nm610 nm650 nm5¢¡¤£ £ ¥§¦©¨ ¨
Spectral Overlap500 nm 660 nm6¢¡¤£ £ ¥§¦©¨ ¨ Spectra View – Raw Data7300 instrumentFAM VIC ® TAMRA ROX (dyes)First cycleLast cycle,Increase offluorescence signalin filter A (Fam orSYBR Green)7¢¡¤£ £ ¥§¦©¨ ¨
Page 1: System IntroductionApplied Biosyste
Page 5 and 6: Allelic Discrimination View Single
Page 7 and 8: Latest Software VersionSDS v1.4 sof
Page 10 and 11: Disposables96-well platesOptical 96
Page 12 and 13: „Help“ Function in SDS Software
Page 14 and 15: Monthly MaintenanceIf the pixel int
Page 16 and 17: After a Lamp Change• Run ROI cali
Page 18: Bi-annual Maintenance• 1) Generat

System Introduction Applied Biosystems 7300 &7500 Real ... - CRN2M

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