System Introduction Applied Biosystems 7300 &7500 Real ... - CRN2M

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After a Lamp Change• Run ROI calibration• using the „ROI Calibration Plate“ from the „Spectralcalibration Kit“ for 7300 & 7500 systems(using the Green Tray for 7000 system)• Run and extract a background• using the „Background Plate“ from the „SpectralCalibration Kit“ or any 96 well plate filled with water.Signal should be:< 72.000 for 7300 / 7500 systems Filter A-D< 90.000 for filter E (only 7500 system)(< 1.200 for the 7000 system)¢¡¤£ £ ¥§¦©¨ ¨ 47Check ROI Mask after Lamp Change1) Open a new Document2) Instrument > Calibrate, select „Block up“3) Select first Filter A, take „Snapshot“4) Click „Generate calibration“ green circles appear5) Save calibration6) Run calibration for each filter- 1024 ms- Block up- Select FiltersA to D or E- Take Snapshot48Please follow the guidelines of the£ ¥§¦©¨ ¨ ¢¡¤£„Installation and Maintenance“ Guide
Instrument Log49¢¡¤£ £ ¥§¦©¨ ¨ Function Test• Perform a System Function Test: select „ALL Tests“Never select„Download Firmware“50¢¡¤£ £ ¥§¦©¨ ¨
Page 1 and 2: System IntroductionApplied Biosyste
Page 3 and 4: Spectral Overlap500 nm 660 nm6¢¡
Page 5 and 6: Allelic Discrimination View Single
Page 7 and 8: Latest Software VersionSDS v1.4 sof
Page 10 and 11: Disposables96-well platesOptical 96
Page 12 and 13: „Help“ Function in SDS Software
Page 14 and 15: Monthly MaintenanceIf the pixel int
Page 18: Bi-annual Maintenance• 1) Generat

System Introduction Applied Biosystems 7300 &7500 Real ... - CRN2M

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