System Introduction Applied Biosystems 7300 &7500 Real ... - CRN2M

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Monthly MaintenanceIf the pixel intensity shows a bright spot in the ROI Inspectorview, and background raw data exceeds 72.000 rfu, check thethermal cycler block for contamination:• Leave the block empty• Open ROI Inspector• Block up• Take Snapshots43¢¡¤£ £ ¥§¦©¨ ¨ ROI Inspector• Open a new document• Instrument > Calibrate2048 msBlock upFilter ASnapshot44If any well shows a bright spot, run abackground run (see following slides)¢¡¤£ £ ¥§¦©¨ ¨
Block Cleaning• Use Water HPLC grade or EtOH p.aa.) Cleaning withcotton budsb.) Cleaning with lint- andfluorescence-free Kimwipes ®45¢¡¤£ £ ¥§¦©¨ ¨ Lamp Status /ReplacementInstrument → Lamp Status/ReplacementNote: Lamp automatically stops 4h after run is completed(if nobody touches the computer)46¢¡¤£ £ ¥§¦©¨ ¨
Page 1 and 2: System IntroductionApplied Biosyste
Page 3 and 4: Spectral Overlap500 nm 660 nm6¢¡
Page 5 and 6: Allelic Discrimination View Single
Page 7 and 8: Latest Software VersionSDS v1.4 sof
Page 10 and 11: Disposables96-well platesOptical 96
Page 12 and 13: „Help“ Function in SDS Software
Page 16 and 17: After a Lamp Change• Run ROI cali
Page 18: Bi-annual Maintenance• 1) Generat

System Introduction Applied Biosystems 7300 &7500 Real ... - CRN2M

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