Human Sertoli Cells & Media - Biocenter

Lonza Walkersville, Inc.www.lonza.comscientific.support@lonza.comScientific Support: 800-521-0390Document# TS-MM-HSE-2305-2 07/09Walkersville, MD 21793-0127 USA© 2009 Lonza Walkersville, Inc.Clonetics ® Human Sertoli Cells & MediaIntroductionHuman male testes contain a population of “nurse”cells, known as Sertoli cells, capable of replicationas undifferentiated cells. The Human Sertoli CellSystem contains Normal Human Sertoli Cells andmedium for their maintenance and growth. Eachsystem can generate sertoli cell cultures forexperimental studies in male infertility, in vitro germcell proliferation models, drug screening, malereproductive toxicology, xeno- and allograftcotransplantation, immune suppression applicationsand apoptotic control mechanisms. They areexcellent tools for gene delivery research, functionalgenomics, drug screening, high throughputscreening and toxicology.Clonetics ® Sertoli Cells and Media are quality testedand guaranteed to give optimum performance.Cell System Components (Need to be purchasedseparately)• One Sertoli Cell Product (Cryopreserved inpassage 3) ≥ 500,000 cells.• One Sertoli Cell Medium BulletKit ® , 500 mlSeGM BulletKit ® (# 00191053) contains one500 ml bottle Sertoli Cell Basal Medium(SeBM) and the following growthsupplements: 25 ml Fetal Bovine Serum (FBS)and 0.5 ml GA-1000 as an SeGMSingleQuots ® Kit (# 00191052).PerformanceRecommended seeding density forsubcultureRecommended feeding intervalTypical time from seeding tosubculture readiness4000 - 5000cells/cm 23 - 4 days7 - 10 daysCharacterization of CellsRoutine characterization of Sertoli cells includestesting for cellular proteins, for seeding efficiency,doubling time and morphology and ≥ 70%confluence by day 12 in culture. Cells test positivefor GATA-4 and Sox-9 at > 70% by flow cytometry.Refer to Certificate of Analysis (COA) for specificQuality Control results.Quality ControlAll cells are performance assayed and test negativefor HIV-1, Hepatitis B & C, mycoplasma, bacteria,yeast and fungi. Cell viability and morphology aremeasured after recovery from cryopreservation. ACertificate of Analysis (COA) for each cell lot isshipped with each order. COAs for all otherproducts are available upon request.Ordering InformationMM-HSE-2305 HSEC - Human SertoliCells00191053 SeGM BulletKit ®SeBM plusSingleQuots ® Kit of GrowthSupplements00191051 SeBM, Sertoli Cell BasalMedium00191052 SeGM SingleQuots ® Kit,Formulates SeBM toSeGM≥500,000cells500 ml500 mlAll trademarks herein are marks of Lonza Groupor its subsidiaries.

Limited Use LicenseSertoli cells are produced for Lonza by MandalMedInc. and are subject to the following limited uselicense:The included biological material, including progenyand derivatives, (collectively referred to as“Material”) is licensed to you under specific terms.You are responsible for ensuring that the terms ofthe license agreement are met.1. GRANTS OF LICENSE: Lonza grants you anontransferable, nonexclusive license to use theMaterial for research.2. NOT FOR HUMAN USE: The Material may notbe used: a) in humans; b) in conjunction withhuman clinical trials; c) in association withhuman diagnostics.3. MATERIAL NOT TRANSFERABLE: You maynot transfer the Material to any other person ororganization4. PATENT NOTICE: Material is under licensefrom MandalMed Inc. Material is covered by USPatent 2009/0028833 A1.When placing an order or for technical support,please refer to the product numbers anddescriptions listed above. For a complete listing ofall Lonza Products, refer to the Lonza website or thecurrent Lonza catalog. To obtain a catalog,additional information or technical service you maycontact Lonza by web, e-mail, telephone, fax or mail.Product WarrantyCULTURES HAVE A FINITE LIFESPAN IN VITRO.Lonza warrants its cells only if SeBM Basal Mediaand SeGM Growth Supplements are used, andthe recommended protocols are followed.Cryopreserved Sertoli Cells are assured to be viableand functional when thawed and maintainedproperly.THESE PRODUCTS ARE FOR RESEARCH USE ONLY. Notapproved for human or veterinary use, for application to humansor animals, or for use in clinical or in vitro procedures.WARNING: Sertoli Cells CONTAIN HUMAN SOURCEMATERIAL, TREAT AS POTENTIALLY INFECTIOUS. In theevent donor testing is not possible, cell products are tested for thepresence of viral nucleic acid from HIV, Hepatitis B Virus, andHepatitis C Virus. Testing can not offer complete assurance thatHIV-1, Hepatitis B Virus, and Hepatitis C Virus are absent. Allhuman sourced products should be handled at the BiologicalSafety Level 2 to minimize exposure of potentially infectiousproducts, as recommended in the CDC-NIH Manual, Biosafety inMicrobiological and Biomedical Laboratories, 1999. If you requirefurther information, please contact your site Safety Officer orScientific Support.All trademarks herein are marks of Lonza Groupor its subsidiaries.