Arts - Buffalo State College
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Arts - Buffalo State College

Arts - Buffalo State College Arts - Buffalo State College

buffalostate.edu
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23.11.2012 Views

Physical Geography, Sciences, and Mathematics Acid-Base Indicators and the Spring Production: Chemistry-Theater Collaboration Joshua Churik, Biology Faculty Mentors: Professor Maria Pacheco, Chemistry, Professor Ann Emo, Theater, and Professor Joy Guarino, Theater As part of a collaborative study with members of the Theater Department, the interaction between commercial (phenolphthalein, bromophenol blue, methyl red) and natural (red cabbage extract) acid-base indicators with various fabric samples (muslin, spandex, nylon and others) will be explored. Fabrics will be tested for ease of absorption of the indicator as well as changes in physical properties upon absorption. The corresponding color changes when the indicator-coated fabric is exposed to either a mild acid or a mild base will be characterized by the type and brightness of the color produced, the rate of appearance of the color and the post exposure color retention on the fabric. The results will be incorporated in the design and construction of costumes to be used in the Theater Department’s Spring Production. The audience will have the opportunity to interact with the performers through the production and will induce color changes in the costumes by exposing the fabrics to mild acids and bases. Presentation Type and Session: Poster VIII Analysis of a Point Mutation (G5W) In the Amino Terminus of Connexin32 Nicholas Vaughn, Biology Faculty Mentor: Professor Martha Skerrett, Biology The gap junction protein connexin 32 (Cx32) was studied using site-directed mutagenesis. Cx32 is expressed in the liver, skin, and brain of mammals where it forms gap junction channels between adjacent cells. As part of a larger class project that involved tryptophan scanning of the Cx32 N-terminus, tryptophan was substituted for glycine at position five of its protein sequence (Cx32 G5W). The function of Cx32 G5W was assessed using the Xenopus oocyte expression system and the two-electrode voltage clamp method. The mutant formed gap junction channels between paired oocytes but the voltage-dependence of the channels was altered and conductance was significantly reduced. In order to better understand the consequences of the G5W substitution, a similar mutation was created in the high-resolution crystal structure of connexin 26 using the “mutagenesis” function in PyMol. In Cx26-based channels the amino terminus folds into the pore and substitution of tryptophan creates an obvious reduction in pore diameter. Presentation Type and Session: Oral – Science, Mathematics, and Technology Analysis of Street Traffic Density Impact On CO 2 and Air Particle Distribution In Beijing, China Robin Tedlock, Geography Faculty Mentor: Professor Tao Tang, Geography and Planning The growing economy in China has drawn many people to the cities. As people have more money, they would like to drive cars. It is estimated that over 1000 cars per day are added to the roads in Beijing, China. This contributed a great impact on air pollution in the city. The current research examines the distribution of gasses and particles across Beijing. Field sampling and measurements were taken at 86 locations across the city over a period of 2 weeks from June 3rd to June 18th 2011. The survey data was then interpolated into map layers using the universal kriging function in ArcGIS. The density of traffic lanes was interpreted from high spatial resolution satellite images. The number of lanes was assigned to the attribute database for each of the street segments. Kernel density of the traffic lanes was analyzed in ArcGIS. The kriging surfaces were then overlayed against a surface representing the density of traffic lanes. The results of this analysis show that areas with higher lane density either having more roads, more lanes, or both, generally coincide with areas that have lower concentrations of CO 2. The only exception is the CBD area, where the high density is coincident with high concentration. This suggests that traffic capacity and the number of cars on the road at a given time may have less of an effect on local air quality than other pollution sources. In the meantime, weather condition of the time may impact the distribution of the pollutants. Presentation Type and Session: Oral – Science, Mathematics, and Technology Beach Sand As a Reservoir For E. coli: Implications of Water Quality Testing Nicole Bennett, Geology Faculty Mentor: Professor Gary Pettibone, Biology Bacterial contamination at freshwater beaches has been an ongoing concern among beach goers and researchers worldwide. Extensive water quality monitoring has been done on public beach waters but few evaluations of the water quality of interstitial water from beach sand have been carried out. A modified AMS gas vapor probe was used to extract water from beach sand and an AMS sand core was used to collect sand samples weekly between June and August, 2011 at two depths along a transect at Bennett Beach. Samples were collected along transects at the water/beach interface and at 6 meters up beach from the shoreline. Escherichia coli were enumerated from water and sand core samples according to EPA Method 1603 (2009). 79% of the interstitial water samples were found to have E. coli densities higher than the EPA standard of 235 CFU per 100mL of water while E. coli levels in beach sand cores exceeded EPA standards in 94% of the samples. Moreover, E. coli levels were 1-3 logs higher in the interstitial water and beach 93

94 Physical Geography, Sciences, and Mathematics sand samples than in lake water adjacent to the beach. There was a significant difference between E. coli densities collected from the beach water and from sand samples taken at the beach/ water interface at a depth of 24-30cm (p-value

94<br />

Physical Geography, Sciences, and Mathematics<br />

sand samples than in lake water adjacent to the beach. There<br />

was a significant difference between E. coli densities collected<br />

from the beach water and from sand samples taken at the beach/<br />

water interface at a depth of 24-30cm (p-value

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