Arts - Buffalo State College

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Fabrication of a Microfluidic Device Containing Multiple Parallel Channels For Fluorescence Sensing Brandon Sligh, Chemistry Faculty Mentor: Professor Jinseok Heo, Chemistry My presentation is about the fabrication of a microfluidic device containing twelve parallel channels for the simultaneous detection of various concentrations of a fluorescent solution. The device consists of an elastic polydimethyl siloxane (PDMS) polymer and a glass slide. This device will significantly save the consumption of chemical reagents requiring less than 10 uL of solution for analysis. Microfabrication is a key technology in the semiconductor industry. For my research the industrial microfabrication method was modified to suit for an ordinary chemistry laboratory that does not have a clean room facility. A photoresist solution was spin-coated on the glass surface. After a drying step, the photoresist layer was exposed to UV light via a photomask and developed in an alkaline solution. The photomask image was designed with AutoCAD and printed on a polyester film with a high-resolution laser printer. The developed photoresist was used a master. PDMS molds were prepared by casting a PDMS prepolymer against the photoresist master and curing in a hot oven. The microfluidic device was completed by sealing the PDMS mold with a clean glass slide. The microfluidic device was tested using calcein, a fluorescent dye. Different concentrations of calcein solutions were filled in each channel of the device and the fluorescence image was obtained using a fluorescence microscope. Calibration curves could be easily constructed by analyzing the fluorescence signal from each channel. This microfluidic device will be used to quickly determine the critical micelle concentration of a surfactant. Presentation Type and Session: Poster VIII Fabrication of Microwell Array For Studying Single Cell Volume Regulation Valerie Fleischauer, Chemistry and Forensic Chemistry Faculty Mentors: Professor Jinseok Heo, Chemistry and Professor Andrea Guiati, Director, All College Honors Program Development of a single cell microwell array is important for studying or screening cells at single cell level in high throughput. Here we report two different methods of fabricating microwell array. For the first method, we formed a hexagonal-close packed single layer of microbeads on a glass surface by slowly evaporating a drop of polystryene bead solution confined in a well defined hydrophilic surface. Then, heating the layer at 240°C for 1 min created good adhesion of the self-assembled layer on the surface. This fused bead layer can be successfully used as a master for making microwell array. The dimension of the microwell was restricted by the sizes of microbeads that were available. In addition, we constructed another master consisting of photoresist. The 25 x 25 microwell array pattern was imprinted on the photoresist-coated glass by exposing it to UV Physical Geography, Sciences, and Mathematics light via photomask and a developer solution was used to reveal the array pattern. The dimensions of each microwell array can be easily controlled by changing the mask design. Microwell arrays were prepared by casting polydimethylsiloxane (PDMS) against either of the two masters. The trapping efficiency of these arrays for the bead and Madin-Darby Canine Kidney (MDCK) cells showed lower than ~10%. Our work is currently in progress to improve the capturing efficiency. Presentation Type and Session: Poster VII Factors Affecting Treatment Efficacy In Post-Mastectomy Lymphedema Patients Shelby Baldwin, AMT 495: Special Project for Applied Mathematics Faculty Mentors: Professor Chaitali Ghosh, Mathematics and Professor Joaquin Carbonara, Mathematics In this day and age, almost all of us are close to or at least know a woman who has had to undergo a mastectomy after being diagnosed with breast cancer. What many of us do not know is the uphill battle they face after this procedure; not only are they recovering from a major surgery but the onset of other conditions can make their daily life almost unbearable. One of these conditions is lymphedema, which is the acute or chronic swelling of a limb due to the lack of proper drainage of the lymph fluid. Post-mastectomy patients suffer from lymphedema in the limb corresponding to the side of their mastectomy resulting in deformity, pain and necrosis of the cutaneous tissue in severe cases. These women seek treatments including massage therapy, bandaging, kinesio taping and prescribed exercises over a course of weeks to reduce the swelling and to restore mobility. This project investigates the relationship between age, affected limb, number of visits, years since mastectomy, type of treatment and reduction in volume of affected limb after treatment, in a cross-sectional study of 45 upper extremity lymphedema patients between the ages of 41 and 93 years, identified from WNY Breast Health, Buffalo, NY. Factors affecting the efficacy of treatment are determined using standard statistical analysis to increase awareness of the odds of recovery from this debilitating condition. Presentation Type and Session: Oral – Science, Mathematics and Technology Finding a Quarterback: Statistically Speaking Breanna Fenski, HON 400: All College Honors Program Faculty Mentor: Professor Andrea Guiati, Director, All College Honors Program The purpose of my research project is to combine my two loves: statistics and sports. The premise of my project is to take an in depth look at a number of quarterbacks from their college playing days through the NFL. Not only did I look at successful professional 97
98 Physical Geography, Sciences, and Mathematics quarterbacks, but also ones that had good college statistics but did not succeed in the NFL. Using several online references, I observed various categories such as completion percentage, touchdowns, and interceptions. Also, I considered other factors such as combine statistics and how they affected their respective teams positively or negatively. Any overall team improvement would be considered a positive impact, and the opposite would be measured as a negative impact. I will use my findings to predict the success of some of the big name players who are entering the draft this year such as Andrew Luck and Robert Griffin III. Presentation Type and Session: Poster I Geochemical Analysis of Associated Granite Bodies From the Eastern Sebago Pluton, Maine Krista Ventura, Geology Faculty Mentors: Professor Gary Solar, Earth Sciences and Science Education and Professor Paul Tomascak, Geochemistry (SUNY Oswego) As part of a large-scale, ongoing project, the research focused on rocks in the northern Appalachians, north of Portland, Maine. Evidence shows partial melting of rocks during their deformation, and the emplacement of associated granite bodies of various sizes. Study of the relations of the mineral patterns and associated granite bodies at several scales is a means of understanding granite magma production, travel and emplacement as granite bodies. My work was focused on the geochemical analysis of granitic rocks from the Eastern Sebago Pluton. Geochemistry is used to help understand the age and composition of the rock source. Field work was done to obtain samples from the field area near Poland, Maine. Two distinct areas were explored for sampling, the Sebago Pluton, and an area to the north that has rocks of similar composition to that of the Sebago Pluton. We conducted new mapping along an E-W corridor along the north contact of the Sebago pluton as part of our lab’s ongoing work on understanding the relation of the pluton to its migmatitegranite complex country rocks reported on previously by students in our lab in the last 5 years (LaFleur, Nyitrai, Bohlen, Naschke). In the summers of 2010 and 2011, we discovered this area of similarity to the pluton that was separated from the pluton by the migmatitegranite complex country rocks. Rocks in the pluton are typically medium-grained, and homogeneous 2-mica granite, whereas the complex outside the pluton is plastically deformed, strongly heterogeneous migmatites, and granites with varying solid-state fabrics. 2011 field work was required in order to both (1) check field data against my 2010 lab results, and to (2) continue mapping in unexplored parts of the field area near Poland, Maine. Geochemistry was performed on selected specimens in the geochemistry laboratory at Syracuse University. Results show positive correlations with previous field data. Presentation Type and Session: Poster VI Growth and Low Temperature Optical Studies of HoMnO 3 Thin Films Anthony Delmont, Physics Faculty Mentor: Professor Ram Rai, Physics We prepared multiferroic HoMnO 3 by a conventional solid-state method. We used high purity Ho 2O 3 and Mn 2O 3 powder samples (and also Ho 2O 3 and MnO 2) to synthesize two different batches of HoMnO 3. The synthesized HoMnO 3 compound was pressed into halfinch diameter pellets using a hydraulic press, and the pellets were used as the target material for the electron-beam system. To fabricate high quality thin films, we deposited HoMnO 3 thin films on different single crystal substrates, such as sapphire, quartz, YSZ, and LiNbO 3, using the electron-beam evaporation system. We studied the growth parameters, such as substrate temperature, oxygen partial pressure, and evaporation rate to improve the quality of the thin films. All thin films with thickness ranging from 100 nm to 300 nm were annealed after each deposition. To characterize our thin film samples, we utilized a dual beam spectrophotometer (190 – 3000 nm) equipped with a liquid nitrogen-cooled cryostat. We also took advantage of our department’s new spectrometer, which utilizes fiber optic cables and is much faster, to take preliminary room temperature transmittance and reflectance on our samples. Our transmittance data, measured at temperatures ranging from 78 K to 490 K, show insulating character with a weak Mn d to d electronic excitation at ~1.7 eV. We also found that the electronic excitations of the HoMnO 3 thin films are weaker than the corresponding excitations in the bulk samples, possibly due to quantum size effects. Presentation Type and Session: Poster VI Has Cazenovia Park Impacted Cazenovia Creek? West Cassenti, Rafael Manenti, and Brittany Carlson, GES 460: Environmental Field Methods and Analysis Faculty Mentor: Professor Elisa Bergslien, Earth Sciences and Science Education Recent flooding and a history of industrial development in South Buffalo, New York have potentially impacted Cazenovia Park. This research is to determine if any contamination of the park has taken place and in contrast if any contamination is occurring due to Cazenovia Park on Cazenovia Creek. The fragile balance of these adjacent entities is in question. Is Cazenovia Creek affecting the remediation of Buffalo’s urban watershed and the park that houses a handful of community destinations, including a playground, bike path, pool house, casino, and a golf course. The research will be conducted on the creek and the park composed of water, soil and creek sediment samples in order to determine if the park is impacting the creek, vice versa, or the possibility of both entities simultaneously impacting the other. We will be testing for temperature, pH levels, and heavy metals, as well as phosphates and nitrates, which is essential in the event that any fertilizers are entering Cazenovia Creek upstream from the park, to see if
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Editor Jill K. Singer, Ph.D. Direct
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Welcome Statements ________________
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Welcome to the 14 th annual Student
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Congratulations to all the student
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‘Year of the Arts’ Events: Warr
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Information Assurance Through Cyber
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Poster Sessions and Displays Butler
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Sooner Rather Than Later: The Role
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Shadows Under the Bed Mallory Schel
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Parent Survey Evaluation and Distri
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Family: Portraits In High Dynamic R
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34 Arts both pianist and pedagogue.
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