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300User Guide for Thresholds and ClassificationsTest typeTest guidelinesOECD EC USEPA OPPTS OtherEvaluation in anaqueous medium ofthe ‗ultimate‘biodegradability oforganic compounds –Method by analysis ofreleased CO 2301C Modified MITI (I)301D Closed bottletestISO 10707 (1994).Water quality –Evaluation in anaqueous medium ofthe ‗ultimate‘biodegradability oforganic compounds –Method by analysis ofbiochemical oxygendemand (closed bottletest)301E Modified OECDscreening test301F Manometricrespirometry testISO 9408 (1991).Water quality –Evaluation in anaqueous medium ofthe ‗ultimate‘biodegradability oforganic compounds –Method bydetermining theoxygen demand in aclosed respirometerOECD 306 (1992).Biodegradability inseawater.OECD. 310 (2006)ReadyBiodegradability –CO 2 in sealed vessels(Headspace Test)Aquatic simulation tests835.3170 Shake flaskdie-away testASTM E 1279-89(95)Standard test methodJanuary 2012 EPA0109
301User Guide for Thresholds and ClassificationsTest typeTest guidelinesOECD EC USEPA OPPTS Otherfor biodegradation bya shake-flask dieawaymethodISO/DIS 14592 (1999)Water quality –Evaluation of theaerobicbiodegradability oforganic compounds atlow concentrations inwater. Part 1: Shakeflask batch test withsurface water orsurfacewater/sedimentsuspensions(22.11.1999)OECD 308 (2002).Aerobic and anaerobictransformation inaquatic sedimentsystemsEC C24 Aerobic andanaerobictransformation inaquatic sedimentsystems835.3180Sediment/watermicrocosmbiodegradability testOECD 309 (2004)Aerobic mineralisationin surface water –simulationbiodegradation testInherent biodegradabillityOECD 302A (1981).Inherentbiodegradability:Modified SCAS test.EC C.12.Biodegradation:Modified SCAS test.Directive 67/548/EEC,Annex V. (1998)OPPTS 835.3210Modified SCAS testASTM E 1625-94Standard test methodfor determiningbiodegradability oforganic chemicals insemicontinuousactivated sludge(SCAS)OECD 302B (1992).Zahn-Wellens/EMPAtestEC C.9.Biodegradation: Zahn-Wellens test. Directive67/548/EEC, Annex V.(1988)835.3200 Zahn-Wellens/EMPA testISO 9888 (1991).Water quality –Evaluation of theaerobicbiodegradability oforganic compounds inan aqueous medium –Static test (Zahn-January 2012 EPA0109
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