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298User Guide for Thresholds and ClassificationsTable 19A.1: Aquatic toxicity test guidelines for chemicals, including mixturesSpeciesTest guideline numberOECD EC USEPA OPPTSAlgae/aquatic plantCrustaceaOECD 201 (2006) Alga,Growth Inhibition TestOECD 221 (2006) Lemnasp. Growth inhibition testEC C.3: Algal InhibitionTest (1992)850.5400 Algal toxicity, Tiers I and II850.4400 Aquatic plant toxicity testusing Lemna spp. Tiers I and II850.4450 Aquatic plants field study,Tier IIIAcuteOECD 202 (2004) Daphniasp. Acute ImmobilisationTestEC C.2: Acute Toxicity forDaphnia (1992)850.1010 Aquatic invertebrate acutetoxicity, test, freshwater daphnids850.1020 Gammarid acute toxicitytest850.1035 Mysid acute toxicity test850.1045 Penaeid acute toxicity testChronicOECD 211 (1998) Daphniamagna Reproduction TestEC C.20: Daphnia magnaReproduction Test (2001)850.1300 Daphnid chronic toxicitytest850.1350 Mysid chronic toxicity testFishAcuteOECD 203 (1992) Fish,Acute Toxicity TestEC C.1: Acute Toxicity forFish (1992)850.1075 Fish acute toxicity test,freshwater and marineChronicOECD 204 (1984) Fish,Prolonged Toxicity Test:14-Day StudyOECD 210 (1992) Fish,Early-Life Stage ToxicityTest850.1400 Fish early-life stage toxicitytestOECD Test Guideline 212(1998) Fish, Short-termToxicity Test on Embryoand Sac-Fry StagesEC C.15: Fish, Short-termToxicity Test on Embryoand Sac-Fry Stages (2001)OECD Test Guideline 215(2000) Fish, JuvenileGrowth TestEC C.14: Fish JuvenileGrowth Test (2001)850.1500 Fish life cycle toxicityJanuary 2012 EPA0109
299User Guide for Thresholds and ClassificationsTable 19A.2: Aquatic toxicity test guidelines for microbial biopesticidesUSEPA OPPTS guidelines885.4000 Background for non-target organism testing of microbial pest control agents885.4200 Freshwater fish testing, Tier I885.4240 Freshwater aquatic invertebrate testing, Tier I885.4280 Estuarine and marine animal testing, Tier I885.4650 Aquatic invertebrate range testing, Tier III885.4700 Fish life cycle studies, Tier III885.4750 Aquatic ecosystem test885.5000 Background for microbial pesticides testing885.5300 Expression in a freshwater environment885.5400 Expression in a marine or estuarine environment19A.3 Abiotic and biotic degradationA large number of test methods are available for evaluating the degradability of a substance in the aquaticenvironment (see Table 19A.3).A pass in one of the ready biodegradability tests will meet the HSNO Act criteria for a substance to beconsidered rapidly degradable. Results from other test methods will usually require further interpretation toassess whether the HSNO Act criteria are met or not.See Appendix 19E for detailed guidance on the interpretation of test results and further information ondegradation testing.Table 19A.3: Test methods for degradability of organic substances in aquatic systemsTest guidelinesTest typeOECD EC USEPA OPPTS OtherReady biodegradability tests301A DOC die-awaytestEC C.4. A to F:Determination ofreadybiodegradability.Directive 67/548/EEC,Annex V. (1992) (arethe same as theOECD 301 tests)835.3110 Readybiodegradability301B CO 2 evolutiontestISO 9439 (1990).Water quality –January 2012 EPA0109
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