Download (PDF) - Metrohm
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New separation columns for ICNew IC columns complete the Metrohm range of products for ionchromatographyMetrohm has included various new IC separation columns in its product range. Inaddition to the favorably-priced Metrosep A Supp 10 (see report on page 14), wepresent three new columns for attractive special applications: Whether anions inbrine, the determination of pharmaceutical ingredients such as paracetamol andcaffeine or the isocratic separation of glycolate and acetate – Metrohm can now offersuitable columns for all these applications.Column specificationsColumn dimensions 250 mm x 4.0 mmSeparation material Polyvinyl alcoholwith quaternary ammoniumgroupsParticle size 5 µmpH range 3...12RecommendedprecolumnMetrosep RP Guard(6.1011.020)Metrosep A Supp 7 – 250 (6.1006.630)The Metrosep A Supp 7 has been specially developed for the simultaneous determinationof oxohalides, standard anions and dichloroacetic acid. However, thisimportant application is by far not the only field of use of this new high-performanceIC column. It is also ideally suitable for the isocratic separation of glycolateand acetate – this means that it is now possible to dispense with costly gradientsystems.The disinfection by-products (DBP) generated in water treatment plants are suspectedto be not only a general health hazard, but could even be carcinogenic.For this reason the oxohalides are the subject of many investigations and standardmethods (e.g. EPA 300.1 Part B, EPA 317.0). Most attention is being givento bromate, which is produced from bromide during the ozonization of drinkingwater.With the new Metrosep A Supp 7 IC column the oxohalides, standard anions anddichloroacetic acid can now be determined reliably and accurately down to the lowppb range. The polyvinyl alcohol separation material with a particle diameter of only5 µm guarantees extremely high plate numbers and thus high chromatographicefficiency. A temperature of 45 °C is required for the separation.A further interesting field of application for the Metrosep A Supp 7 is monitoring thecooling water in nuclear power plants. Too high ion concentrations accelerate thecorrosion of the piping systems and must therefore be avoided at all costs. Thismeans that an accurate monitoring system that functions 100% is absolutely essential.Apart from acetate, which is added to the cooling water as an additive, anyglycolate present as a contaminant also has to be determined.Glycolate and acetate are separated isocratically, that is without the use of agradient system that would otherwise be required for this application (see chromatogrambelow). Applying the preconcentration technique, it is possible to reliablydetermine concentrations as low as 100 ppt in the original solution.Determination of glycolate, acetate and formate besidesthe standard anions using the Metrosep A Supp 7 – 250IC column. As can be seen from the enlarged section ofthe chromatogram, glycolate and acetate are separatedat the baseline.Eluent: 3.5 mmol/L Na 2CO 3Flow rate: 0.8 mL/minSample: Standard containing 0.5 ppm fluoride,chloride, nitrite, bromide, nitrate, phosphate,sulfate; 5 ppm glycolate, acetate,formateTemperature: 45 °CDetection: Conductivity12 Metrohm Information Issue 2/2005
New separation columns for ICMetrosep A Supp 8 – 150 (6.1017.010)Determination of anions in brine samples? Previously this application could onlybe realized by carrying out complicated and time-consuming sample preparationsteps. With the new high-capacity Metrosep A Supp 8 «salt column» the ion chromatographicdetermination is now possible without any sample purification. Theanions are detected photometrically; our new 844 UV/VIS Compact IC is ideal forthis application.If eluents with high ion concentrations (10% NaCl solution) are used then samplesolutions containing up to 30% NaCl can be injected. In this way detection limits inthe low ppb range can be achieved.Column specificationsColumn dimensions 150 mm x 4.0 mmSeparation material Styrene/divinylbenzenecopolymer with quaternaryammoniumgroupsParticle size 5 µmpH range 0...14Recommendedprecolumn Metrosep A Supp 8Guard (6.1017.500)[Determination of nitrite, bromide and nitrate in brine usingthe Metrosep A Supp 8 – 150 separation column(with Metrosep A Supp 8 Guard precolumn).Eluent: 100 g/L NaClFlow rate: 1.0 mL/minTemperature: 22 °CDetection: UV at 215 nmResults: Nitrite 50 ppbBromide 1.4 ppmNitrate 120 ppbProntoSIL 120-5-C18 AQ (6.1008.100)In the field of determination of pharmaceutical ingredients, the 844 UV/VIS CompactIC opens up a multitude of new applications for ion chromatography. With theProntoSIL 120-5-C18 AQ a suitable IC separation column is now available for theseapplications.The separation and determination of broad-spectrum antibiotics (cefazolin, amoxicillin,ampicillin, etc.) or painkillers such as acetylsalicylic acid (aspirin) andparacetamol (known as acetaminophen in North America) can be carried out withthe 844 UV/VIS Compact IC and ProntoSIL 120-5-C18 AQ just as fast and reliablyas determining the caffeine content of cola drinks.Column specificationsColumn dimensions 150 mm x 4.0 mmSeparation material Silica gel C18Particle size 5 µmpH range 2...9RecommendedprecolumnProntoSIL 120-5-C18AQ Guard (6.1008.110)Determination of paracetamol, caffeine and 4-hydroxybenzoicacid at three different wavelengths using theProntoSIL 120-5-C18 AQ IC column.Eluent: 20 mmol/L H 2SO 4in acetonitrile/water(15:85 v/v)Flow rate: 1.0 mL/minSample: Standard containing 20 ppm paracetamol,caffeine, 4-hydroxybenzoic acidTemperature: 22 °CDetection: UV at 220 nm, 254 nm, 270 nmMetrohm Information Issue 2/2005 13
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- Page 5: Third-party instruments in tiamofro
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- Page 10 and 11: IC-MS systemA powerful combination:
- Page 14 and 15: Metrosep A Supp 10In focus: the new
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- Page 20 and 21: 6.0262.100 Ecotrode Plus6.0262.100
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New separation columns for ICMetrosep A Supp 8 – 150 (6.1017.010)Determination of anions in brine samples? Previously this application could onlybe realized by carrying out complicated and time-consuming sample preparationsteps. With the new high-capacity Metrosep A Supp 8 «salt column» the ion chromatographicdetermination is now possible without any sample purification. Theanions are detected photometrically; our new 844 UV/VIS Compact IC is ideal forthis application.If eluents with high ion concentrations (10% NaCl solution) are used then samplesolutions containing up to 30% NaCl can be injected. In this way detection limits inthe low ppb range can be achieved.Column specificationsColumn dimensions 150 mm x 4.0 mmSeparation material Styrene/divinylbenzenecopolymer with quaternaryammoniumgroupsParticle size 5 µmpH range 0...14Recommendedprecolumn Metrosep A Supp 8Guard (6.1017.500)[Determination of nitrite, bromide and nitrate in brine usingthe Metrosep A Supp 8 – 150 separation column(with Metrosep A Supp 8 Guard precolumn).Eluent: 100 g/L NaClFlow rate: 1.0 mL/minTemperature: 22 °CDetection: UV at 215 nmResults: Nitrite 50 ppbBromide 1.4 ppmNitrate 120 ppbProntoSIL 120-5-C18 AQ (6.1008.100)In the field of determination of pharmaceutical ingredients, the 844 UV/VIS CompactIC opens up a multitude of new applications for ion chromatography. With theProntoSIL 120-5-C18 AQ a suitable IC separation column is now available for theseapplications.The separation and determination of broad-spectrum antibiotics (cefazolin, amoxicillin,ampicillin, etc.) or painkillers such as acetylsalicylic acid (aspirin) andparacetamol (known as acetaminophen in North America) can be carried out withthe 844 UV/VIS Compact IC and ProntoSIL 120-5-C18 AQ just as fast and reliablyas determining the caffeine content of cola drinks.Column specificationsColumn dimensions 150 mm x 4.0 mmSeparation material Silica gel C18Particle size 5 µmpH range 2...9RecommendedprecolumnProntoSIL 120-5-C18AQ Guard (6.1008.110)Determination of paracetamol, caffeine and 4-hydroxybenzoicacid at three different wavelengths using theProntoSIL 120-5-C18 AQ IC column.Eluent: 20 mmol/L H 2SO 4in acetonitrile/water(15:85 v/v)Flow rate: 1.0 mL/minSample: Standard containing 20 ppm paracetamol,caffeine, 4-hydroxybenzoic acidTemperature: 22 °CDetection: UV at 220 nm, 254 nm, 270 nm<strong>Metrohm</strong> Information Issue 2/2005 13
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