ACE C18-AR and C18-PFP - Hplc.eu

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®ACEHPLC / UHPLC ColumnsSelectivity: The Key to Chromatographic Peak Resolution8EfficiencySelectivity Retention3.02.5R s =√ N4-1k1+k~40% ~80% ~10%Resolution (R s)2.01.51.0Nk0.5From the examples, selectivity hasthe greatest impact on increasingpeak resolution0.01.00 1.05 1.10 1.15 1.20 1.250 5000 10000 15000 20000 250000 5 10 15 20 25NkZhao, J.H. and P.W. Carr. Analytical Chemistry, (1999) 71, 2623-2632
®ACEHPLC / UHPLC ColumnsWhich Factors a Affect Selectivity?9MOSTInfluenceIsocratic SeparationsColumn stationary phaseGradient SeparationsAll parameters for isocraticOrganic modifierGradient steepnesspH (ionised analytes only) k*% Organic modifierDwell volumeBuffer selectionColumn dimensionsColumn temperatureBuffer concentrationLEASTInfluencek*tG F 85 V Sma Adapted from ‘Introduction to Modern Liquid Chromatography”, 3 rd Edition, Snyder, Kirkland, Dolan, 2010, p.29, Wiley & Sons
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ACE C18-AR and C18-PFP - Hplc.eu

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