ACE C18-AR and C18-PFP - Hplc.eu
ACE C18-AR and C18-PFP - Hplc.eu
ACE C18-AR and C18-PFP - Hplc.eu
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
®<strong>ACE</strong>HPLC / UHPLC ColumnsSelectivity: The Key to Chromatographic Peak Resolution8EfficiencySelectivity Retention3.02.5R s =√ N4-1k1+k~40% ~80% ~10%Resolution (R s)2.01.51.0Nk0.5From the examples, selectivity hasthe greatest impact on increasingpeak resolution0.01.00 1.05 1.10 1.15 1.20 1.250 5000 10000 15000 20000 250000 5 10 15 20 25NkZhao, J.H. <strong>and</strong> P.W. Carr. Analytical Chemistry, (1999) 71, 2623-2632