ACE C18-AR and C18-PFP - Hplc.eu

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®ACEHPLC / UHPLC ColumnsThe Importance of N, k and For Resolution6Double Retention Factor (e.g., decrease solvent strength):k = 4 8√ NR s =4-1k1+kR SR S14 2.01.11810,000 1.1 18R s = 1.8~10% IncreaseR s = 2.0Slight improvement in resolution has led to increased analysis time
®ACEHPLC / UHPLC ColumnsThe Importance of N, k and For Resolution7Increase Selectivity (e.g., change column): = 1.1 1.2R s =√ N4-1k1+kR SR S1 1.214 10,0004 1.2 14 3.3R s = 1.8Can now shorten run time with shorter column and/orfaster flow rate~80% IncreaseR s = 3.3Significant opportunity to speed up for modest change in selectivity
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ACE C18-AR and C18-PFP - Hplc.eu

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