ACE C18-AR and C18-PFP - Hplc.eu
ACE C18-AR and C18-PFP - Hplc.eu
ACE C18-AR and C18-PFP - Hplc.eu
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
®<strong>ACE</strong>HPLC / UHPLC ColumnsLosses in Performance Due to Incorrect Column Fitting47mAUCorrectly fitted column300 1.5363.147 5.6658.22625020015010050N 5%h= 12,800 platesPW 5%h= 0.291 minA s= 0.94Correctly fittedcolumns make themost of your column<strong>and</strong> system02 4 6 8 10minIncorrectly fitted columnmAU300 1.5373.160 5.7132502001501005008.3132 4 6 8 10< ~ 0.5mm gapN 5%h= 9,850 platesPW 5%h= 0.335 minA s= 0.84min Incorrectly connectedcolumns lead toreduced efficiency,reduced peaksymmetry, <strong>and</strong>possibly, leaksLoss of ~23% for NLoss of ~11% for A s