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ACE C18-AR and C18-PFP - Hplc.eu

ACE C18-AR and C18-PFP - Hplc.eu

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®<strong>ACE</strong>HPLC / UHPLC ColumnsEffect of Pressure on Selectivity <strong>and</strong> Retention Factor43Initial 2µm <strong>and</strong> 3µm data aresimilar (A, B)Scalability looks goodRetention <strong>and</strong> selectivity seento change with pressure (BE)Agilent 1290, 50 x 2.1 mm (constant flow <strong>and</strong> restrictor capillary used)Mobile phase: A=0.1% FA in water: B=0.1% FA in MeOH (51:49 v/v)Flow Rate: 0.21 ml/min, Temperature: 40 °CK= Ketoprofen; S= Sulindac; N=Naproxena Fallas et al. / J. Chromatogr. A 1209 (2008) 195–205

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