ACE C18-AR and C18-PFP - Hplc.eu

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®ACEHPLC / UHPLC ColumnsThe Importance of N, k and For Resolution4Typical separation:N = 10,000 platesk = 3.8 / 4.2 (4.0 mean) = 1.1R SR S14 1.81.11410,000 1.1 14R s =√ N4-1k1+kWhich looks like
®ACEHPLC / UHPLC ColumnsThe Importance of N, k and For Resolution5Double Efficiency (e.g., 5 m 2.5 m):N = 10,000 20,000 platesR s =√ N4-1k1+kR SR S14 2.61.11420,000 1.1 14R s = 1.8~40% IncreaseR s = 2.6Opportunity to optimize further, e.g. reduce column length to speed up
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ACE C18-AR and C18-PFP - Hplc.eu

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