ACE C18-AR and C18-PFP - Hplc.eu
ACE C18-AR and C18-PFP - Hplc.eu
ACE C18-AR and C18-PFP - Hplc.eu
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®<strong>ACE</strong>HPLC / UHPLC Columns<strong>ACE</strong> ® Excel <strong>C18</strong>-<strong>PFP</strong> Selectivity & Throughput (Gradient)39Aim: obtain R s≥ 1.7 in shortest possible time for mixture<strong>ACE</strong> 5µm <strong>C18</strong>100 x 4.6 mm1 ml/min, t G= 29 minmax pressure: 92 bar40 min cycle timeR s= 1.81325476, 22.49 minHPLC: 5µm <strong>C18</strong>22.49 mins<strong>ACE</strong> Excel 2µm <strong>C18</strong>50 x 2.1 mm0.6 ml/min, t G= 5 minmax pressure: 367 bar9 min cycle time<strong>ACE</strong> Excel 2 μm <strong>C18</strong>-<strong>PFP</strong>30 x 2.1 mm2.5 ml/min, tG = 0.7 minmax pressure: 914 bar1 min cycle time00R s = 1.80R s= 1.51327, 0.61 minR s = 1.912345R s= 1.760.5 15 42.5 57R s = 1.7UHPLC: 2µm <strong>C18</strong>‐<strong>PFP</strong>0.61 mins6, 4.17 minUHPLC: 2µm <strong>C18</strong>4.17 mins1.5 2 2.5 3 3.5 425~ x25QuickerminR s ≥ 1.705 10 15 20 251, aspirin; 2, phenacetin; 3, 1,3‐dinitrobenzene ; 4, ethyl benzoate; 5, nimesulide; 6, ibuprofen; 7, indomethacin.min
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