ACE C18-AR and C18-PFP - Hplc.eu
ACE C18-AR and C18-PFP - Hplc.eu
ACE C18-AR and C18-PFP - Hplc.eu
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®<strong>ACE</strong>HPLC / UHPLC Columns<strong>ACE</strong> ® Excel <strong>C18</strong>-<strong>PFP</strong> Selectivity & Throughput (Isocratic)38Aim: obtain R s≥ 1.7 in shortest possible time for mixtureWaters XBridge 5µm <strong>C18</strong>150 x 4.6 mm1.00 ml/min163 barR s= 1.71 23HPLC: 5µm <strong>C18</strong>13.6 mins(L/d p= 3)413.60 minWaters Acquity 1.7µm BEH <strong>C18</strong>50 x 2.1 mm0.21 ml/min246 bar3R s= 1.11 24.69 min4UPLC: < 2µm <strong>C18</strong>4.75 mins(L/d p= 2.9)To maintain R s <strong>and</strong>reduce run time,keep L / dp ratioconstant~ x23Quicker<strong>ACE</strong> Excel 2μm <strong>C18</strong>-<strong>PFP</strong>30 x 2.1 mm1.30 ml/min492 barUHPLC: <strong>ACE</strong> Excel 2µm <strong>C18</strong>‐<strong>PFP</strong>< 1 min2 4 6 8 10 12 14 16min1R s = 3.4230.65 min4R s= 1.9Using UHPLC <strong>and</strong> selectivity, itis possible to dramatically improveresolution allowing shorter columns& increased flow ratesSample: 1) 1,2,dimethoxybenzene, 2) 1,3‐dimethoxybenzene, 3) 1,3,5‐trimethoxybenzene, 4) toluene (reference).Mobile phase 50:50 MeOH / H 2 O; Temperature 40°C; 254 nmAll trademarks are recognised...comparative separations may not be representative of all applications
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