ACE C18-AR and C18-PFP - Hplc.eu
ACE C18-AR and C18-PFP - Hplc.eu
ACE C18-AR and C18-PFP - Hplc.eu
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®<strong>ACE</strong>HPLC / UHPLC Columns<strong>ACE</strong> ® <strong>C18</strong>-<strong>PFP</strong> Selectivity1,2345,67<strong>ACE</strong> 3 <strong>C18</strong>Hypersil GOLD 3 μm <strong>PFP</strong>Hydrophobicreference8<strong>C18</strong> or <strong>PFP</strong> mechanisms alonenot enough to fully resolve themethoxybenzene isomers2213425186 732456 7 8ReducedHydrophobicity<strong>ACE</strong> 3 <strong>C18</strong>-<strong>PFP</strong><strong>ACE</strong> <strong>C18</strong>-<strong>PFP</strong> mechanismcombines hydrophobicity,shape selectivity, dipole-dipole<strong>and</strong> π-π interactionsElution order, retention <strong>and</strong>selectivity all seen to differ5 10 15 20 25Minutes1) 1,2,3‐trimethoxybenzene, 2) 1,2,4‐trimethoxybenzene, 3) 1,2‐dimethoxybenzene, 4) 1,4‐dimethoxybenzene 5) methoxybenzene, 6) 1,3‐dimethoxybenzene,7) 1,3,5‐trimethoxybenzene, 8) toluene (ref) Mobile phase 50:50 v/v MeOH / H 2 O; Column= 150 x 4.6 mm id; 1.00 ml/min; 40C; 254 nmAll trademarks are recognised...comparative separations may not be representative of all applicationsPowerful positional isomer <strong>and</strong>shape selectivity
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