ACE C18-AR and C18-PFP - Hplc.eu
ACE C18-AR and C18-PFP - Hplc.eu
ACE C18-AR and C18-PFP - Hplc.eu
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®<strong>ACE</strong>HPLC / UHPLC ColumnsAromatic Functionality: π – π Interactions15A type of electron donor-acceptor interactionOriginates from π systems in unsaturated functionalgroups on analytes <strong>and</strong> the stationary phaseTypes of π-π interaction can be manipulated formaximum effect (orthogonality) in phase design− eg phenyl: electron rich ring on the stationary phase also actsas π-base <strong>and</strong> interacts well with electron poor analytes− eg <strong>PFP</strong>: electron poor ring on the stationary phase also actsas π-acid <strong>and</strong> interacts well with electron rich analytes
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