ACE C18-AR and C18-PFP - Hplc.eu
ACE C18-AR and C18-PFP - Hplc.eu
ACE C18-AR and C18-PFP - Hplc.eu
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®<strong>ACE</strong>HPLC / UHPLC ColumnsInfluencing Selectivity – Bonded Phase Effects / Basic Analytes10<strong>ACE</strong> <strong>C18</strong> – Increase Retention<strong>ACE</strong> CN – Elution Order<strong>ACE</strong> C8 (start point)<strong>ACE</strong> C4 – Decrease Retention<strong>ACE</strong> Phenyl – Elution OrderUse ultra high purity silica for good chromatography <strong>and</strong> reproducibilityColumn: 250 x 4.6mm, 5m Mobile phase: 80:20 MeOH/25mM KH 2 PO 4 (pH 6.0) Flow: 1.00ml/minComponents; 1: Norephedrine, 2: Nortriptyline, 3: Toluene, 4: Imipramine, 5: Amitriptyline Wavelength: 215nm
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