Pharmacognostical Studies & Phytochemical Evaluation of the Stem ...

Indo Global Journal of Pharmaceutical Sciences, 2013; 3(1): 58-66Fluorescence AnalysisThe results were summarized in Table no 2.Phytochemical screening: Refer Table 3.Thin Layer Chromatographic AnalysisThe leaves extract showed three distinct spots with differentintensities. The colors are yellow, yellowish green and violet incolor respectively. The resultant R f values were summarized inTable -4.Powdered drug Visible/Day light UV 254 nm (short) UV 365 nm (long)Powder drug as such Light brown Brown Yellowish brownPowder + Methanol Light brown Yellowish brown Brownish blackPowder + 1% glacial acetic acid Brown Dark brown Blackish brownPowder +10% NaOH Yellowish brown Dark yellowish brown Bluish brownPowder + dil. NH 3 Yellowish brown Light brown BrownPowder + Conc. HNO 3 Brown Blackish brown Dark brownPowder+ dil.NH 3 +Conc.HNO 3 Yellowish brown Light brown Blackish brownPowder +1M H 2 SO 4 Brown Dark brown Yellowish blackPowder +1M HCl Brownish yellow Brown Dark brownPowder + 10% FeCl 3 Reddish brown Light brown Brownish yellowPowder +Acetone+ Methanol Light brown Brown BlackPowder +10% Iodine Yellowish brown Dark brown Blackish brownTable 2 Shows fluorescence analysis of powdered bark of E. officinalis Gaertn.Sl. No. Plant Constituents EXTRACTS (STEM BARKS)Petroleum Chloroform Acetone Methanol Waterether(40°-60°c)1. Alkaloids: _ + + + _2. Carbohydrates _ _ + + +3. Glycosides _ _ + + +4. Steroids: + + + + _5. Fats and Oils: _ _ + + _6. Tannins and Phenolic _ _ + + +compounds:7. Proteins: _ _ + + +9. Gums: _ _ + + _10. Mucilages: _ _ _ _ _11. Flavonoids: _ _ + + +12. Lignin’s: _ _ _ _ _13 Triterpenes: _ _ _ _ _Table 3 Shows phyto-chemical screenings of successive extracts of stem barks of Emblica officinalis Gaertn. Here, ‘+’ indicatespresence ‘-’ indicates absenceeISSN: 2249 102363