Zebron™ Inferno™ - Phenomenex

NEW!NEW!ZebronInfernoRugged High Temperature GC ColumnsZB-1HTZB-5HTZB-35HTZB-XLB-HT

Manufacturing Award WinningSolutions for GC AnalysisIn the field of gas chromatography, not much has changedin the last 15 years. However, technology is our passionand Phenomenex is gaining international recognitions forits GC products that are specially designed for today's mostchallenging methods.For chemists struggling with high boilers, contaminants, or carry-overs, Phenomenexdeveloped the Zebron Inferno line. This line of GC columns haverevolutionized GC analysis by allowing chemists to run their analysis at oventemperatures that were never before attainable - all without having to use ametal column!The Zebron ZB-1HT and ZB-5HT Inferno columns are rugged, mid-polaritycolumns that allow chemists to push their standard analysis up to 430 ˚C forincreased lab productivity and improved performance. The newly developedZebron ZB-35HT and ZB-XLB-HT Inferno columns provide high temperaturecapability up to 400 ˚C for those using more polar columns.If Zebron does not provide youwith equivalent separationsas compared to any other GCcolumn of the same phaseand dimensions, send in yourcomparative data within 45days and keep the Zebroncolumn for FREE!

ZebronInfernoRugged High Temperature GC Analysis45678101112131525Challenges with Traditional GC ColumnsAdvanced Solutions for High Temperature AnalysisLow BleedIncreased Column LifetimeIdeal for High Boiling CompoundsBake-off Contaminants and Eliminate Carry-overs!See What You Have Been Missing!Widely Used PhasesMid-Polar PhasesApplicationsEnvironmental............ 16Petrochemical............ 21Biodiesel.................... 22Food and Beverage.... 23Chemical................... 24Pharmaceutical.......... 25Ordering InformationZB-1HT...................... 12ZB-5HT...................... 12ZB-35HT.................... 14ZB-XLB-HT................. 14GC Accessories.......... 25(Zebron ZB-1HT and ZB-5HT)(Zebron ZB-35HT and ZB-XLB-HT)NEW!NEW!NEW!© 2010 Phenomenex, Inc. All rights reserved.Phenomenex | Tel: 310-212-0555 | Fax: 310-328-7768 | Email: info@phenomenex.com | Web: www.phenomenex.com3

Challenges with Traditional GC ColumnsStandard fused silica columns - Shorter lifetimeUnlike the Zebron Inferno GC columns, most fused silica columns are notdeveloped to handle prolong exposure to temperatures above 360 °C.At high temperatures, fused silica GC columns:• Become inflexible and brittle• Break easily and are costly to replace• Require more system downtime to replace columns• Can’t separate high molecular weight compoundsDeterioration of a standard column at high temperaturesMetal columns – Difficult to useThough metal columns have higher temperature limits than capillary columns, theyare more difficult to use.Metal columns:• Inflexible and hard to use• Require special tubing cutters for installation• Prone to leaks from expansion and contraction• Have active surface, giving poor peak shapes for acids and bases• Incompatible with MS detectors4Phenomenex | Tel: 310-212-0555 | Fax: 310-328-7768 | Email: info@phenomenex.com | Web: www.phenomenex.com

Advanced Solutions forHigh Temperature AnalysisZebron Inferno columns offer enhanced performancewhere other columns fail. The two critical factors thatallow for this are:1. Advanced ESC Bonding TechnologyAt high temperature ranges, the stability of standard GC columns willdeteriorate, resulting in increased bleed. Zebron’s Engineered SelfCross-linking (ESC) bonding technology reinforces the stationaryphase for enhanced column durability and extremely low bleed levels.The result - our Inferno columns allow for the flexibility to perform GCanalysis at high temperatures while providing low bleed and longercolumn lifetime.2. Special High Temperature Polyimide CoatingStandard polyimide resin pyrolyzes at temperatures above 360 °C,making the tubing unstable. The Zebron Inferno columns utilize a specialdark polyimide resin that shows minimal thermal degradation even attemperatures up to 430 °C.* This results in longer column lifetime atelevated temperatures.Phase Temperature LimitZB-1HT 430 °CZB-5HT 430 °CNEW!NEW!ZB-35HT 400 °CZB-XLB-HT 400 °CFlexible Even at 430 °C!**The Zebron Inferno columns remainflexible and easy to work with, evenafter being exposed to many hoursat extreme temperatures. Its hightemperature stability offers theflexibility of a non-metal columnwhile providing long lifetime, lowbleed, and low activity.* Zebron ZB-1HT and ZB-5HT Inferno columns have an upper temperature limit of 430 °C.Zebron ZB-35HT and ZB-XLB-HT Inferno columns have an upper temperature limit of 400 °C.** Evaluated by performing 185 programmed temperature runs, total 23 hours at 430 ˚C.Polyimide tubing was still flexible as shown here.Phenomenex | Tel: 310-212-0555 | Fax: 310-328-7768 | Email: info@phenomenex.com | Web: www.phenomenex.com5

Greater Performance forHigh Boiling Compounds• Better peak shape• Improved separation• More analytes detectedZebron Inferno GC columns have the inertness and temperature stability neededfor separating high molecular weight compounds. These high boiling compoundsrequire a thermally rugged column with low bleed levels. Zebron Inferno columnshave the high temperature stability and durability to provide optimal separation ofthese compounds.Great Separation of High Boiling Hydrocarbons (ASTM Method D6352)1614825 6 7 931 338 10 1211 13414 1527 291617 18 19 212325320 2224262830323435 36 373839 40414243444546Easily Elute HydrocarbonsUp To C90Zebron’s ZB-1HT Inferno can operateat 430 °C, allowing C90 to elute ontemperature ramp to meet ASTMrequirements1474849 56 585051 52 54 5753 55App ID 16148010 20 minColumn: Zebron ZB-1HT InfernoDimensions: 5 meter x 0.53 mm x 0.10 μmPart No.: 7AK-G014-02Injection: On-Column @ 43 °C, 0.1 μLCarrier Gas: Helium @ 4.4 mL/min (constant flow)Oven Program: 40 °C for 0.5 min to 430 °C @ 20 °C/min for 10 minDetector: FID @ 430 °CNote: Sample was a combination of PolyWax 655 and retention timemarkers C8-C40 in CS2/ChloroformSample:1. C102. C113. C124. C135. C146. C157. C168. C179. Pristane10. C1811. Phytane12. C1913. C2014. C2115. C2216. C2317. C2418. C2519. C2620. C2721. C2822. C2923. C3024. C3125. C3226. C3327. C3428. C3529. C3630. C3731. C3832. C3933. C4034. C4235. C4436. C4637. C4838. C5039. C5240. C5441. C5642. C5843. C6044. C6245. C6446. C6647. C6848. C7049. C7250. C7451. C7652. C7853. C8054. C8255. C8456. C8657. C8858. C908Phenomenex | Tel: 310-212-0555 | Fax: 310-328-7768 | Email: info@phenomenex.com | Web: www.phenomenex.com

Bake-off Contaminants and Eliminate Carry-overs!• Achieve better reproducibility• Extend column lifetime• Attain sharper peak shapesEliminate Problems Associated with Dirty SamplesDirty samples can linger on a GC column, eventually changing theselectivity and shortening the lifetime of a column. Since Zebron Inferno columns have extremely high temperature limits, it allowsfor the use of more aggressive column baking steps to removepersistent contaminants, thus prolonging column lifetimes.Hydrocarbon contaminants revealedusing 18778 FID starting at 5 minutes16306Hydrocarbon contaminantsare not detected by ECD!12Mineral OilFID @435 °CApp ID 187780 2 4 6 8 10 12 14 16 18 minECD @380 °C34Mineral OilApp ID 163064Conditions for App ID 16306:6Column: Zebron ZB-5HT InfernoDimensions: 30 meter x 0.32 mm x 0.10 μmPart No.: 7HM-G015-02Injection: Split 52:1 @ 330 °C , 1 μLCarrier Gas: Helium @ 1.9 mL/min (constant flow)Oven Program: 150 °C for 2 min to 420 °C @ 20 °C/min for 4.5 minDetector: ECD @ 380 °CSample: 1. TCMX2. DCOB3. Aroclor 10164. Mineral OilNote: Aroclor 1016 @ 100 ppb; Mineral Oil @ 1 % in isooctane8 10 12 14 16 18 min10 Phenomenex | Tel: 310-212-0555 | Fax: 310-328-7768 | Email: info@phenomenex.com | Web: www.phenomenex.com

Available in Widely Used PhasesZB-1HT Inferno • Temperature Limits: -60 to 400/430 °C (Isothermal/TPGC)*• First non-metal columns stable to 430 °C• Provides true boiling point separation for hydrocarbon distillation methods• Individually tested for low bleed, MS certifiedIf Zebron does not provide youwith equivalent separations ascompared to any other GCcolumn of the same phaseand dimensions, send in yourcomparative data within 45days and keep the Zebroncolumn for FREE!Alternative to any 100 % Dimethylpolysiloxane High-Temperature Phase:• DB-1ht • MXT-1 SimDist • Petrocol 2887 • CP-SimDistColumn ProfileLow5PolarityBleedTemperature LimitsStabilityApplications• High Boiling Petroleum Products• Simulated Distillation Methods• Long-chained Hydrocarbons• High Molecular Weight Waxes• Polymers/Plastics• Motor Oils• Diesel FuelHighOrdering InformationZebron ZB-1HT Inferno GC ColumnsID(mm) df(µm) Temp. Limits °C Part No. Price5-Meter0.53 0.10 -60 to 400/430 7AK-G014-02 $ 31515-Meter0.25 0.10 -60 to 400/430 7EG-G014-02 3350.32 0.10 -60 to 400/430 7EM-G014-02 3650.32 0.25 -60 to 400/430 7EM-G014-11 3650.53 0.15 -60 to 400 7EK-G014-05 36520-Meter0.18 0.18 -60 to 400/430 7FD-G014-08 48030-Meter0.25 0.10 -60 to 400/430 7HG-G014-02 5500.25 0.25 -60 to 400/430 7HG-G014-11 5500.32 0.10 -60 to 400/430 7HM-G014-02 5900.32 0.25 -60 to 400/430 7HM-G014-11 5900.53 0.15 -60 to 400 7HK-G014-05 645ZB-1HT Test MixPart No.: AG0-5155Price: $ 55ZB-5HT Inferno • Temperature Limits: -60 to 400/430 °C (Isothermal/TPGC)*• First non-metal columns stable to 430 °C• Individually tested for low bleed, MS certifiedExtend column lifetime. Add a Z-guard to your next Zebron GC order.If you need a 5 in. cage, simply add a (-B) after the part number, e.g., 7HG-G014-11-B. Some exceptions may apply.*0.53 mm ID columns are rated to 400 ˚C max operational temperature.Alternative to Any 5 %-Phenyl-95 % Dimethylpolysiloxane High Temperature Phase:• DB-5ht • VF-5ht • HT-5 • Stx-5HT • XTI-5HTColumn ProfileLowPolarityBleedTemperature LimitsStability8Applications• High Boiling Petroleum Products• Triglycerides• Simulated Distillation Methods• Diesel Fuel• Long-chained HydrocarbonsHigh• Motor Oils• Polymers/Plastics• Surfactants• High Molecular Weight WaxesOrdering InformationZebron ZB-5HT Inferno GC ColumnsID(mm) df(µm) Temp. Limits °C Part No. Price15-Meter0.25 0.10 -60 to 400/430 7EG-G015-02 $ 3350.25 0.25 -60 to 400/430 7EG-G015-11 3300.32 0.10 -60 to 400/430 7EM-G015-02 3650.32 0.25 -60 to 400/430 7EM-G015-11 3650.53 0.15 -60 to 400 7EK-G015-05 36515-Meter with 2-Meter Spliced Guard (0.53 mm ID)0.32 0.10 -60 to 400/430 7EM-G015-02-GST 43020-Meter0.18 0.18 -60 to 400/430 7FD-G015-08 48030-Meter0.25 0.10 -60 to 400/430 7HG-G015-02 5500.25 0.25 -60 to 400/430 7HG-G015-11 5500.32 0.10 -60 to 400/430 7HM-G015-02 5900.32 0.25 -60 to 400/430 7HM-G015-11 5900.53 0.15 -60 to 400 7HK-G015-05 645ZB-5HT Test MixPart No.: AG0-5155Price: $ 55Extend column lifetime. Add a Z-guard to your next Zebron GC order.If you need a 5 in. cage, simply add a (-B) after the part number, e.g., 7HG-G015-11-B. Some exceptions may apply.*0.53 mm ID columns are rated to 400 ˚C max operational temperature.12Phenomenex | Tel: 310-212-0555 | Fax: 310-328-7768 | Email: info@phenomenex.com | Web: www.phenomenex.com

Two New Innovative Mid-Polar Phases:Zebron ZB-35HT and ZB-XLB-HT Inferno Unlike standard mid-polar GC columns that fail at high oven temperatures, theNEW Zebron ZB-35HT and ZB-XLB-HT Inferno columns have the durability andruggedness to perform well at extreme temperatures. With an astonishing uppertemperature limit of 400 ˚C, both columns will provide the separation of highboiling compounds at extremely low bleed levels.Last 2x longer!Zebron ZB-XLB-HTVarian ® VF-XmsJ&W ® DB-XLBRestek ® Rtx ® -XLB0 20 40 60 80 100Hours at 400 °CZebron Inferno Provides the Lowest Bleed †Dimensions: 30 meter x 0.25 mm x 0.25 µmInjection: Split 20:1 @ 200 °C, 1 µLCarrier Gas: Helium @ 1.7 mL/min (constant flow)Oven Program: 100 °C to 320 °C @ 30 °C/min for 5 min to 340 °C @ 20 °C/min for 5min to 360 °C @ 20 °C/min for 5 min to 380 °C @ 20 °C/min for 5 minto 400 °C @ 20 °C/min for 5 min to 100 °C @ 30 °C/min for 8 minDetector: FID @ 405 °CRestek® Rtx®-35mspA604020Varian® VF-35msAgilent® J&W® DB-35msPhenomenex® Zebron ZB-35HT†All columns were new, never used, prior to this testing. Columns were purchased either directly from the original manufacturer orthrough an authorized distributor. All testing was carefully controlled to ensure conditions were similar for all columns involved.2.5 5 7.5 10 12.5 15The comparative data is not representative of every application.17.5 20 22.5 minAgilent and J&W are registered trademarks of Agilent Technologies, Inc.Varian is a registered trademark under license to Varian, Inc.Restek and Rtx are registered trademarks of Restek Corporation.Phenomenex | Tel: 310-212-0555 | Fax: 310-328-7768 | Email: info@phenomenex.com | Web: www.phenomenex.com13

Suited for aWide RangeApplicationsofFor Many Industries:Environmental........pp.16-20Petrochemical.........p.21Biodiesel ...............p.22Food/Beverage.......p.23Chemicals..............p.24Pharmaceuticals.....p.24Dual Column AnalysisEPA 552.2......... p.16EPA 8081.......... p.18EPA 8151........... p.18EPA 8270.......... p.17High Molecular Weight CompoundsHeavy PAHs............................ p.19Long chained hydrocarbons..... p.21Oils........................................ pp.21, 23Triglycerides............................ p.23Dirty SamplesOils.......... pp.21, 23Low Level AnalysisEnvironmental contaminants.......pp.16-20Pesticides..................................pp.16-20Biodiesel...................................p.22Pharmaceuticals........................p.24

Environmental ApplicationsEPA Method 552.2 - Haloacetic Acids187918Column: Zebron ZB-XLB-HT Inferno Dimensions: 30 meter x 0.25 mm x 0.25 µmPart No.: 7HG-G024-11Dual Column Analysis12187908Column: Zebron ZB-35-HT Inferno Dimensions: 30 meter x 0.25 mm x 0.25 µmPart No.: 7HG-G025-1112132 457691011App ID 1879112,3456791110App ID 187900 10 20 min0 10 20 minConditions for both columns:Injection: Split 5:1 @ 250 °C, 1 µLCarrier Gas: Helium @ 4.0 mL/min (constant flow)Oven Program: 40 °C for 1 min to 55 °C @ 5 °C/min for 5 min to 70 °C @10 °C/min for 9 min to 250 °C @ 30 °C/min for 2 minDetector: ECD @ 300 °CNote: Analytes are methylated and 100 ppb in isooctane.Sample: 1. Chloroacetic Acid2. Bromoacetic Acid3. Dichloroacetic Acid4. Dalapon5. Trichloroacetic Acid6. 1,2,3-Trichloropropane7. Bromochloroacetic Acid8. Bromodichloroacetic Acid9. Dibromoacetic Acid10. Chlorodibromoacetic Acid11. 2,3-Dibromopropionate12. Tribromoacetic AcidDIN Method 51527: Aroclors 1242 DIN Method 51527: Aroclors 124218177181791 2 312,342142156 78109111213 14 15 16 17 1819 20App ID 181775811127916,176 1013 14 1815 19 20App ID 181796 8 10 12 14 16 18 20 22 minColumn: Zebron ZB-XLB-HT Inferno Dimensions: 30 meter x 0.25 mm x 0.25 µmPart No.: 7HG-G024-11Injection: Spilt 2:1 @ 250 ˚C, 1 µLCarrier Gas: Helium @ 1.5 mL/min (constant flow)Oven Program: 50 °C for 0.5 min to 210 °C @ 40 °C/min for 3 min to230 °C @ 30 °C/min for 5 min to 250 °C @ 30 °C/minfor 5 min to 320 °C @ 40 °C/min for 2 minDetector: ECD @ 350 °CNote: Total concentration of aroclors was 90 ppm in isooctane.Sample:1. TCMX2. BZ# 313. BZ# 284. BZ# 525. BZ#1016. BZ# 777. BZ# 1238. BZ# 1189. BZ# 15310. BZ# 11411. BZ# 10512. BZ# 13813. BZ# 12614. BZ# 16715. BZ# 15616. BZ# 18017. BZ# 15718. BZ# 17019. BZ# 16920. BZ# 18921. DCB10 12 14 16 18 20 22 24 minColumn: Zebron ZB-35HT Inferno Dimensions: 30 meter x 0.25 mm x 0.25 µmPart No.: 7HG-G025-11Injection: Spiltless @ 250 ˚C, 1 µLCarrier Gas: Helium @ 0.5 mL/min (constant flow)Oven Program: 50 °C for 0.5 min to 220 °C @ 30 °C/min to 320 °C @ 6 °C/min for 2 minDetector: ECD @ 350 °CSample: 1. TCMX2. BZ# 313. BZ# 284. BZ# 525. BZ# 1016. BZ# 777. BZ# 1238. BZ# 1189. BZ# 15310. BZ# 11411. BZ# 10512. BZ# 13813. BZ# 12614. BZ# 16715. BZ# 15616. BZ# 18017. BZ# 15718. BZ# 16919. BZ# 17020. BZ# 18921. DCB16Phenomenex | Tel: 310-212-0555 | Fax: 310-328-7768 | Email: info@phenomenex.com | Web: www.phenomenex.com

Petrochemical ApplicationsMotor OilParaffin Wax16071Column: Zebron ZB -1HT Inferno Dimensions: 30 meter x 0.25 mm x 0.10 µmPart No.: 7HG-G014-02Injection: On-Column @ 153 °C, 1 µLCarrier Gas: Helium @ 1 mL/min (constant flow)Oven Program: 150 °C to 400 °C at 14 °C/min for 6 minDetector: FID @ 400 °CNote: Sample was 1 % in dichloromethaneSample: Mobil ® 1 10W-30 Fully Synthetic Motor OilColumn: Zebron ZB-5HT Inferno Dimensions: 15 meter x 0.32 mm x 0.10 μmPart No.: 7EM-G015-02Injection: Direct on column @ 43 °C, 0.01 µLCarrier Gas: Helium @ 1.9 mL/min (35 cm/sec)(constant flow)Oven Program: 40 °C for 2 min to 430 °C @ 20 °Cfor 10 minDetector: FID @ 430 °CSample: Paraffin WaxApp ID 16071Only 1.5 %retention differenceafter 23 hours inextreme conditions4 th injection136 th injectionApp ID 160890510 1520 min8 10 12 14 16 18 20 22minASTM Methods D2887 and D6352 on One ColumnASTM D2887 and D6352 both set standard test methods for boilingrange determination of petroleum fractions by GC. While ASTM D2887is used for products having a final boiling point of 538 °C or lower,ASTM D6352 is used for determining petroleum distillate fractions witha boiling point (BP) up to 700 °C. The Zebron Inferno columns makesimulated distillation analysis easier and less expensive. Typically youwould have to use a traditional fused silica column for ASTM D2887 anda metal column for ASTM D6352. Zebron Inferno columns provide arobust solution for both analyses.ASTM Method D635216148ASTM Method D2887-971235 6 7 931 338 10 1211 13414 1527 291617 18 19 21232520 2224262830323435 36 373839 40414243444546474849 56 585051 52 54 5753 5516217App ID 161481 23456 78910Stable analysis for standardhigh temperature simulateddistillation analysis11 1213 14 15 16 17App ID 16217010 20 minColumn: Zebron ZB-1HT Inferno Dimensions: 5 meter x 0.53 mm x 0.10 μmPart No.: 7AK-G014-02Injection: On-Column @ 43 °C, 0.1 μLCarrier Gas: Helium @ 4.4 mL/min (constant flow)Oven Program: 40 °C for 0.5 min to 430 °C @ 20 °C/min for 10 minDetector: FID @ 430 °CNote: Sample was a combination of PolyWax 655 and retention time markers C8-C40 in CS2/Chloroform.Sample:1. C102. C113. C124. C135. C146. C157. C168. C179. Pristane10. C1811. Phytane12. C1913. C2014. C2115. C2216. C2317. C2418. C2519. C2620. C2721. C2822. C2923. C3024. C3125. C3226. C3327. C3428. C3529. C3630. C3731. C3832. C3933. C4034. C4235. C4436. C4637. C4838. C5039. C5240. C5441. C5642. C5843. C6044. C6245. C6446. C6647. C6848. C7049. C7250. C7451. C7652. C7853. C8054. C8255. C8456. C8657. C8858. C902 4 6 8 10 12 minColumn: Zebron ZB-1HT Inferno Dimensions: 15 meter x 0.53 mm x 0.15 μmPart No.: 7EK-G014-05Injection: On-Column @ 38 ºC, 0.1 μLCarrier Gas: Helium @ 10 mL/min(constant flow)Oven Program: 35 ºC for 1 min to 360 ºC @25 ºC/minDetector: FID @ 375 ºCSample:1. Hexane2. Heptane3. Octane4. Nonane5. Decane6. Undecane7. Dodecane8. Tetradecane9. Hexadecane10. Octadecane11. Eicosane12. Tetracosane13. Octacosane14. n-Dotriacontane15. n-Hexatriacontane16. Tetracontane17. TetratetracontanePhenomenex | Tel: 310-212-0555 | Fax: 310-328-7768 | Email: info@phenomenex.com | Web: www.phenomenex.com21

Biodiesel ApplicationsFree and Total Glycerin in BiodieselThe Zebron ZB-1HT Inferno column is ideal for biodiesel analysis because it is stable at the elevated temperaturesneeded to resolve and remove high boiling triglycerides. While standard polyimide resin degrades at temperaturesabove 380 °C, the Inferno columns utilize a polyimide resin material that shows minimal thermal breakdown, evenat programmed temperatures up to 430 °C– the highest thermal stability of any non-metal GC column!ASTM Method D6584: Free and Total Glycerin in B-100 Biodiesel Methyl EstersImprovedGlycerolResolution1234510 20 min6High Sensitivityfor TrioleinUltra LowBleed at 380 ˚C78910App ID 16211Column: Zebron ZB-5HT Inferno Dimensions: 15 meter x 0.32 mm x 0.10 μm + 2 meter x 0.53 mm Z-GuardPart No.: 7EM-G015-02Injection: On-Column @ 53 ºC, 1 μLCarrier Gas: Helium @ 3.0 mL/min (constant flow)Oven Program: 50 ºC for 1 min to 180 ºC @ 15 ºC/min to 230 °C @ 7 ºC/minto 380 ºC @ 30 ºC/min for 10 minDetector: FID @ 380 ºCNote: A 2.0 m x 0.53 mm Guard Column was connected to the analytical columnper ASTM method requirements.Sample: 1. Glycerol2. Butanetriol (ISTD1)3. Esters4. Monoglycerides5. 1-Monooleoyl-rac-glycerol6. Tricarpin (ISTD2)7. Diglycerides8. 1,3-Diolein9. Triglycerides10. Triolein1815725181582511343476 89App ID 181576789App ID 181580 5 10 15 20 25 30 minColumn: Zebron ZB-35HT Inferno Dimensions: 15 meter x 0.25 mm x 0.10 µmPart No.: 7EG-G025-02Injection: On-Column @ 53 °C, 0.1 µLCarrier Gas: Helium @ 1.3 mL/min (constant flow)Oven Program: 50 °C for 1 min to 180 °C @ 15 °C/min to 230 °C@ 7 °C/min to 380 °C @ 30 °C/min for 10 minDetector: FID @ 380 °CNote: A 30 cm x 0.5 mm ID guard column was connected to the analyticalcolumn per ASTM method requirementsSample: 1. Butanetriol (ISTD1)2. Ester3. Monoglycerides4. 1-Monooleoyl-rac-glycerol5. Tricarpin (ISTD2)6. Diglycerides7. 1,3-Diolein8. Triglycerides9. Triolein0 5 10 15 20 25 30 minColumn: Zebron ZB-XLB-HT Inferno Dimensions: 15 meter x 0.25 mm x 0.10 µmPart No.: 7HG-G024-11Injection: On-Column @ 53 °C, 0.10 µLCarrier Gas: Helium @ 1.3 mL/min (constant flow)Oven Program: 50 °C for 1 min to 180 °C @ 15 °C/min to 230 °C @7 °C/min to 380 °C @ 30 °C/min for 10 minDetector: FID @ 380 °CNote: A 30 cm x 0.5 mm ID guard column was connected to the analyticalcolumn per ASTM method requirementsSample: 1. Butanetriol (ISTD1)2. Ester3. Monoglycerides4. 1-Monooleoyl-rac-glycerol5. Tricarpin (ISTD2)6. Diglycerides7. 1,3-Diolein8. Triglycerides9. Triolein22Phenomenex | Tel: 310-212-0555 | Fax: 310-328-7768 | Email: info@phenomenex.com | Web: www.phenomenex.com

Food and Beverage ApplicationsMelamine in Baby Formula1826521,23,43 416054Butter TriglyceridesComplete analysis in lessColumn: Zebron ZB-XLB-HT Inferno than half the time of theDimensions: 15 meter x 0.25 mm x 0.25 µmFDA methodPart No.: 7EG-G024-11Injection: On-Column @ 103 °C, 1 µLCarrier Gas: Helium @ 1.4 mL/min (constant flow)Oven Program: 100 °C for 0.5 min to 320 °C @ 25 °C/minDetector: Mass Selective (MSD) @ 325 °CSample: Analytes are 200 ng / 100 µL in BSTFA / Pyridine (1:1)5 6 7 8 9 min1. Cyanuric Acid 13C3 (IS)2. Cyanuric Acid3. Melamine 13C3 15N3 (IS)4. MelamineApp ID 18265App ID 16054Column: Zebron ZB-5HT Inferno Dimensions: 15 meter x 0.32 mm x 0.10 μmPart No.: 7EM-G015-02Injection: On-Column @ 103 ºC, 2 μLCarrier Gas: Helium @ 1.8 mL/min (constant flow)Oven Program: 100 ºC to 400 ºC @ 14 ºC/min for 10 minDetector: FID @ 400 ºCSample: Butter1020 30 minSterols from Olive Oil: UnderivatizedSterols from Olive Oil: Derivatized16753416749431325App ID 16753App ID 16749125.566.5 77.5 min6.56.06.5 7.07.5 minColumn: Zebron ZB-5HT Inferno Dimensions: 30 meter x 0.25 mm x 0.10 µmPart No.: 7HG-G015-02Injection: Splitless @ 350 °C, 0.5 µLCarrier Gas: Helium @ 2 mL/min (constant flow)Oven Program: 220 °C to 350 °C @ 15 °C/minDetector: FID @ 350 °CSample: 1. Campesterol2. Stigmasterol3. b-Sitosterol4. Betulin (IS)Column: Zebron ZB-5HT Inferno Dimensions: 30 meter x 0.25 mm x 0.10 µmPart No.: 7HG-G015-02Injection: Splitless @ 350 °C, 0.5 µLCarrier Gas: Helium @ 2 mL/min (constant flow)Oven Program: 220 °C to 350 °C @ 15 °C/min.Detector: FID @ 350 °CNote: Sterols derivatized via BSTFA:TMCS; 99:1 in pyridineSample: 1. Cholesterol2. Campesterol3. Stigmasterol4. b-Sitosterol5. Betulin (IS)Phenomenex | Tel: 310-212-0555 | Fax: 310-328-7768 | Email: info@phenomenex.com | Web: www.phenomenex.com23

Chemical ApplicationsAmine and Nitrosamine187737App ID 18773123, 45 685 10 15 20 25minColumn: Zebron ZB-35HT Inferno Dimensions: 30 meter x 0.25 mm x 0.25 µmPart No.: 7HG-G025-11Injection: Split 50:1 @ 350 ºC, 1 μLCarrier Gas: Helium @ 2.1 mL/min (constant flow)Oven Program: 140 °C to 400 °C @ 10 °C/minDetector: FID @ 410 ºCNote: Application courtesy of Northeastern Chemical CompanySample: 1. MW 135 Amine2. MW 163 Amine3. MW 178 Amine4. MW 208 Amine5. MW 164 Nitrosamine6. MW 178 Nitrosamine7. MW 192 Nitrosamine8. MW 208 NitrosamineDiethylene Glycol Divinyl Ether187702App ID 187707.6 7.8 8 8.2 8.4 8.6 8.8 9 9.2 9.4minColumn: Zebron ZB-35HT Inferno Sample: 1. Isopar CDimensions: 30 meter x 0.25 mm x 0.25 μm2. Diethylene glycol divinyl etherPart No.: 7HG-G025-113. Isobornyl acrylateInjection: Split 40:1 @ 250 ºC, 1 µLCarrier Gas: Helium @ 1.4 mL/min (constant flow)Oven Program: 40 °C for 0.5 min to 150 °C @ 10 °C/minto 310 °C @ 25 °C/min for 2 minDetector: FID @ 320 ºCNote: Application courtesy of Northeastern Chemical CompanyPharmaceutical/DrugsDrug Screen16227113152425262 35, 648 9 1012711141617181920 21 22232728App ID 162275.5 6.0 6.5 7.0 7.5 8 8.5 9 9.5 10 10.5 minColumn: Zebron ZB-1HT Inferno Dimensions: 30 meter x 0.25 mm x 0.10 µmPart No.: 7HG-G014-02Injection: Split 15:1 @ 250 °C, 1 µLCarrier Gas: Helium 1.1 mL/min (constant flow)Oven Program: 50 °C for 1 min to 320 °C @ 25 °C/min for 3 minDetector: MSD @ 310 °C; 45-450 amuNote: Analytes were 25 ppmSample:1. Nicotine2. Contaminant3. Acetaminophen4. Allobarbital5. Aprobarbital6. Ibuprofen7. Butabarbital8. Phenacetin9. Amobarbital10. Pentobarbital11. Meprobomate12. Secobarbital13. Caffeine14. Methyl Benzilate15. Benzphetamine16. Hexobarbital17. Dimenhydrinate18. Doxylamine19. Phenobarbital20. Methapyrilene21. Chlorpheniramine22. Bromopheniramine23. Cocaine24. Chlorcyclizine25. Codeine26. Diazepam27. Hydrocodone28. Oxymorphone24Phenomenex | Tel: 310-212-0555 | Fax: 310-328-7768 | Email: info@phenomenex.com | Web: www.phenomenex.com

Cool-Lock NutCapillary GC Installation with the Cool-Lock Nut• Avoid burning your fingers again – cools with the oven®• No Wite-Out or Tipp-Ex ® needed• Achieve the proper installation depth each and every time• No need for wrench with hand-tightened connections12Insert column through columnnut and ferrule. Insert the end ofthe column and column nut intothe bottom of the Cool-Lock NutInstallation Gauge.Hand-tighten the column nut intothe gauge until the ferrule starts tocompress. Adjust the length of thecolumn to the desired length.GC AccessoriesPatent PendingOrdering InformationCool-Lock GC Capillary Nut For Agilent GC Systems*Part No. Description Unit PriceAG0-8319 Cool-Lock GC Capillary Nut For Use Withea $ 75Short-Style FerrulesAG0-8320 Cool-Lock GC Capillary Nut For Use Withea 75Long-Style FerrulesAG0-8349 Cool-Lock Nut Installation Gauge ea 69Replacement FerrulesAG0-4701 GC Capillary Ferrules Graphite 1/16 in. to 0.5 mm ID 10/pk 39AG0-4704 GC Capillary Ferrules Graphite 1/16 in. to 0.8 mm ID 10/pk 39*Guaranteed fit for Aglilent 5850, 5890, 6850, 6890 GC systems34Unscrew the installation gauge andinstall the nut and column into theinstrument.Make sure Cool-Lock nut is in thefull extension position to achieve thecorrect pre-measured position, andallow for proper leak checking.Patent PendingCool-Lock GC Capillary Nut For Shimadzu Systems**Part No. Description Unit PriceAG0-8419 Cool-Lock GC Capillary Nut For Use Withea $ 75Short-Style FerrulesAG0-8420 Cool-Lock Nut Installation Gauge ea 69**Guaranteed fit for Shimadzu 2010 and 2014 GC systemsGuardian : Integrated Guard Columns• Eliminates the potential for leaks• Extends column life• Improves analyte focusing for low boiling compounds• Aggressively tested to ensure deactivationTraditional Union Connected Guard ColumnPotential for leaksIncreased activityHassle to installvs.Guardian Integrated Guard ColumnContinuous deactivation for betterpeak shape and inertnessSeamless guard transition,no connection hassles andno potential for leaksOrdering InformationGuardian: Integrated Guard ColumnGC Phase Dimensions 5 m Guardian Part No. PriceZebron ZB-1HT 30 meter x 0.25 mm x 0.10 df (μm) 7HG-G014-02-GGA $ 610Zebron ZB-5HT 30 meter x 0.25 mm x 0.10 df (μm) 7HG-G015-02-GGA 610Zebron ZB-5HT 30 meter x 0.25 mm x 0.25 df (μm) 7HG-G015-11-GGA 610Phenomenex | Tel: 310-212-0555 | Fax: 310-328-7768 | Email: info@phenomenex.com | Web: www.phenomenex.com25

GC AccessoriesInlet Base Seals• Prevents analyte adsorption• Improves detection sensitivity• Improves method reproducibility• Fits all Agilent 5890/6890 split/splitless injection portsOrdering InformationGold inlet Base SealsPart No. Description Similar to Mfr. No.* Unit PriceStandard, single groove for splitless applications, 0.8 mm dia. inlet holeAG0-7518 Gold Inlet Base Seal, splitless (single groove), 0.8 mm 18740-20885 2/pk $ 75AG0-7519 Gold Inlet Base Seal, splitless (single groove), 0.8 mm 18740-20885 10/pk 305High Split Flow, cross groove for split applications, 0.8 mm dia. inlet holeAG0-7520 Gold Inlet Base Seal, split (double groove/cross), 0.8 mm 5182-9652 2/pk 79AG0-7521 Gold Inlet Base Seal, split (double groove/cross), 0.8 mm 5182-9652 10/pk 309Replacement Inlet Seal WashersAG0-8397 Inlet Seal Washers, Gold plated, for Agilent GC injection port - 12/pk 50Stainless Steel Base SealsPart No. Description Similar to Mfr. No.* Unit PriceStandard, single groove for splitless applications, 0.8 mm dia. inlet holeAG0-8393 Stainless Steel Inlet Base Seal, splitless (single groove), 0.8 mm 18740-20880 2/pk $ 46AG0-8394 Stainless Steel Inlet Base Seal, splitless (single groove), 0.8 mm 18740-20880 10/pk 200High Split Flow, cross groove for split applications, 0.8 mm dia. inlet holeAG0-8395 Stainless Steel Inlet Base Seal, split (double groove/cross), 0.8 mm 5182-9652 2/pk 55AG0-8396 Stainless Steel Inlet Base Seal, split (double groove/cross), 0.8 mm 5182-9652 10/pk 210Replacement Inlet Seal WashersAG0-7522 Inlet Seal Washers, Stainless Steel, for Agilent GC injection port 5061-5869 12/pk 35Injection Port Inlet Liners• Fit standard instrument manufacturers’ inlets• Popular designs at excellent prices• Reduce analyte discrimination• Improve reproducibility and results• Clean, non-adsorptive/non-reactive surfaceOrdering InformationDimensionsID x L x OD(mm)Material*(deactivated)Quartz Wool(Y/N)Similar toManufacturerNumber** Part No. Unit PriceDescriptionGC Model No.Agilent Technologies (HP)Single Taper Direct Connect with Side Hole (top) 5880/5980/6890 4 x 78.5 x 6.3 B (y) N 21055 AG0-7850 5/pk $ 295Single Taper Direct Connect with Side Hole (bottom) 5880/5980/6890 4 x 78.5 x 6.3 B (y) N G1544-8073020771AG0-7851 5/pk 295Split / Splitless Focus Liner with wool 5880/5890/6890 4 x 78.5 x 6.3 B (y) Y 210-4004 AG0-7515 5/pk 90ShimadzuSplitless Liner 9A/15A/16A 3.4 x 139 x 5.0 B (y) N 20749 AG0-4669 5/pk 145Thermo Scientific (Finnigan)Splitless / Single Taper Liner /Trace 8000 series 5 x 105 x 8.0 B (y) N 45350033 AG0-7852 5/pk 130* B= Borosilicate; Deactivated = Yes (y) or No (n). ** Similar to but not always an exact equivalent to the original manufacturer’s product.iAdditional GC accessories are available.For more information contact our GC Specialists at GCspecialist@phenomenex.com.26Phenomenex | Tel: 310-212-0555 | Fax: 310-328-7768 | Email: info@phenomenex.com | Web: www.phenomenex.com

SeptaSilicone Rubber Inlet Septa• Advanced silicone formulations reduce coring andincrease septum lifetime• Enhanced durability and re-sealing capabilities• Precision formed and cut to exacting specifications• Reduced needle damage• General purpose and specialty low bleed, hightemperaturesepta availablePhenoRed -400Fire brick red, with a 50 durometer rating, this specially formulatedhigh-temperature, low-bleed silicone rubber septum is designed andconditioned for high sensitivity analysis where the demands for low bleedand heat stability are critical. Say goodbye to ghost peaks! Temperatureratedto 400 °C.PhenoGreen -400Long life, high-temperature green septum, rated to 400 °C. GuideRightthrough-hole to guide the needle during injection.GuideRight Injection Hole SeptaThe GuideRight through-hole guides the needle during injection.Septum performance and lifetime increase while downtime due to bentneedles or dirty injection ports decreases. Choose from either hightemperaturePhenoGreen or PhenoRed, both rated to 400 °C.Ordering InformationSilicone Rubber SeptaGC AccessoriesPart No. Description Unit PricePhenoRed 3 ⁄8 in. (9.5 mm) DiameterAG0-4690 PhenoRed-400 Injector Septa 50/pk $ 70AG0-4691 PhenoRed-400 Injector Septa 100/pk 130PhenoRed 7 ⁄16 in. (11 mm)AG0-4696 PhenoRed-400 Injector Septa 50/pk 70AG0-4697 PhenoRed-400 Injector Septa 100/pk 140Ordering InformationGuideRight Injection Hole SeptaPart No. Description Unit PricePhenoGreen 3 ⁄8 in. (9.5 mm) Diameter 400 ˚CAG0-7874 PhenoGreen 400 Injector Septa 50/pk $ 69PhenoGreen 7 ⁄16 in. (11 mm) Diameter 400 ˚CAG0-7875 PhenoGreen 400 Injector Septa 50/pk 70PhenoRed 3 ⁄8 in. (9.5 mm) Diameter 400 ˚CAG0-7916 PhenoRed 400 Injector Septa 50/pk 70PhenoRed 7 ⁄16 in. (11 mm) Diameter 400 ˚CAG0-7917 PhenoRed 400 Injector Septa 50/pk 75Long FerrulesGraphite Ferrules• High-purity graphite significantly reduces ferrule bleed• Special construction minimizes "flaking"• Stable to 450 °C - excellent for high temperature use• Not for use with GC/MS transfer linesiAdditional GC accessories are available.For more information contact our GC Specialists at GCspecialist@phenomenex.com.Ordering InformationGraphite Ferrules, Rated to 450 ˚CPart No. Description Unit Price0.4 mm Ferrule IDAG0-4698 Graphite Ferrule 1•16 in. to 0.4 mm 10/pk $ 39AG0-4699 Graphite Ferrule 1•16 in. to 0.4 mm 50/pk 1350.5 mm Ferrule IDAG0-4701 Graphite Ferrule 1•16 in. to 0.5 mm 10/pk 39AG0-4702 Graphite Ferrule 1•16 in. to 0.5 mm 50/pk 1350.8 mm Ferrule IDAG0-4704 Graphite Ferrule 1•16 in. to 0.8 mm 10/pk 39AG0-4705 Graphite Ferrule 1•16 in. to 0.8 mm 50/pk 135Terms and ConditionsSubject to Phenomenex Standard Terms & Conditions, which may be viewed atwww.phenomenex.com/TermsAndConditions.TrademarksZebron, Inferno, Cool-Lock, Guardian, Engineered Self Cross-linking, ESC, PhenoGreen, PhenoRed, and GuideRight are trademarks ofPhenomenex, Inc. Cool-Lock Nut is patent pending by Phenomenex. Agilent and J&W are registered trademarks of Agilent Technologies, Inc.Varian is a registered trademark under license to Varian, Inc. Restek and Rtx are registered trademarks of Restek Corporation. Mobil is a registeredtrademark of Exxon Mobil Corporation. Wite-Out and TIPP-EX are registered trademarks of BIC Corporation.DisclaimerComparative separations may not be representative of all applications.Cool-Lock Nut is U.S. patent pending by Phenomenex, Inc.© 2010 Phenomenex, Inc. All rights reserved.If Zebron does not provide youwith equivalent separationsas compared to any other GCcolumn of the same phaseand dimensions, send in yourcomparative data within 45days and keep the Zebroncolumn for FREE!Phenomenex | Tel: 310-212-0555 | Fax: 310-328-7768 | Email: info@phenomenex.com | Web: www.phenomenex.com27

Australiat: 02-9428-6444f: 02-9428-6445auinfo@phenomenex.comAustriat: 01-319-1301f: 01-319-1300anfrage@phenomenex.comPRSRT STDU.S. POSTAGEPAIDTORRANCE, CAPERMIT NO. 185BR69520609_usBelgiumt: +31 (0)30-2418700f: +31 (0)30-2383749beinfo@phenomenex.comCanadat: (800) 543-3681f: (310) 328-7768info@phenomenex.comDenmarkt: 4824 8048f: 4810 6265nordicinfo@phenomenex.comFinlandt: 09-4789 0063f: +45 4810 6265nordicinfo@phenomenex.comFrancet: 01 30 09 21 10f: 01 30 09 21 11franceinfo@phenomenex.comGermanyt: 06021-58830-0f: 06021-58830-11anfrage@phenomenex.comIrelandt: 01 247 5405f: +44 1625-501796eireinfo@phenomenex.comItalyt: 051 6327511f: 051 6327555italiainfo@phenomenex.comLuxembourgt: +31 (0)30-2418700f: +31 (0)30-2383749nlinfo@phenomenex.comMexicot: (55) 5018 3791f: (310) 328-7768tecnicomx@phenomenex.comNetherlandst: 030-2418700f: 030-2383749nlinfo@phenomenex.comNew Zealandt: 09-4780951f: 09-4780952nzinfo@phenomenex.comNorwayt: 81 00 20 05f: +45 4810 6265nordicinfo@phenomenex.comPuerto Ricot: (800) 541-HPLCf: (310) 328-7768info@phenomenex.comUnited Kingdomt: 01625-501367f: 01625-501796ukinfo@phenomenex.comAll other countries:Corporate Office USAt: (310) 212-0555f: (310) 328-7768info@phenomenex.comwww.phenomenex.comPhenomenex products are available worldwide. For the distributor in your country,contact Phenomenex USA, International Department at international@phenomenex.com411 Madrid AvenueTorrance, CA 90501-1430USA