measurements of chromosome aberration at tomato plants
measurements of chromosome aberration at tomato plants
measurements of chromosome aberration at tomato plants
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D1-D3, while the control registered 0,88%, <strong>at</strong> L27Sgenotype the value were between 0,12-0,24% for D-D3and 0,25% for control.Regarding the percentage <strong>of</strong> cells with an<strong>at</strong>elophaseswith abnormalities the values are higherthan the one registered for metaphases. At genotypeFrancesca, for example, <strong>at</strong> <strong>plants</strong> regener<strong>at</strong>ed from invitro cultures the percentage <strong>of</strong> cells with abnormalitiesin ana-telophases ranged between 1,02-1,35 %, whilethe control registered 1,96%. The same genotype had0,26% from cells in metaphases with abnormalitieswhile the control had only 0,41%.ConclusionsThe results obtained in our experiment provedth<strong>at</strong> in the “in vitro” conditions tested, the types andfrequency <strong>of</strong> chromosomal <strong>aberr<strong>at</strong>ion</strong> are similar withthe control. No other genetic abnormality <strong>of</strong> the tissuecultured<strong>plants</strong> was observed suggesting th<strong>at</strong> geneticfidelity <strong>of</strong> tissue cultured <strong>plants</strong> can be maintained ifappropri<strong>at</strong>e plant growth regul<strong>at</strong>ors are used with lessnumber <strong>of</strong> subcultures in the multiplic<strong>at</strong>ion stage.The cultiv<strong>at</strong>ion <strong>of</strong> tom<strong>at</strong>oes shoot tips onnutritive medium modified with Kinetin and BAPallows the regener<strong>at</strong>ion <strong>of</strong> new <strong>plants</strong> with a stablegenetic m<strong>at</strong>erial th<strong>at</strong> shows little genetic variability.This variability manifested <strong>at</strong> cellular level through thedifferent types <strong>of</strong> chromosomal abnormalities does notexceed the n<strong>at</strong>ural variability present also on <strong>plants</strong>germin<strong>at</strong>ed in “ex vitro” conditions.The main types <strong>of</strong> abnormalities in the rootcells <strong>of</strong> tom<strong>at</strong>oes are ana-telophases with bridges,metaphases with lagging <strong>chromosome</strong>s, expelled<strong>chromosome</strong>s or ring <strong>chromosome</strong>s, multipolar an<strong>at</strong>elophases,as well as binucle<strong>at</strong>e cells and interphaseswith micro-nucleuses.References1. Li M., M. Zhang, 1991 - Technology for plant<strong>chromosome</strong> research. Northwest Forest Univ. Press,Shenyiang, China. p. 31–39.2. Budiman, M.A., L. Mao, T. Wood and R.A.Wing. 2000. A Deep-Coverage Tom<strong>at</strong>o BAC Libraryand Prospects Toward Development <strong>of</strong> an STCFramework for Genome Sequencing. GenomeResearch 10:129-136.3. Mao, L., D. Begum, S.A. G<strong>of</strong>f, R.A. Wing.2001. Sequence and Analysis <strong>of</strong> the Tom<strong>at</strong>oJOINTLESS Locus. Plant Physiology: 126:1331-1340.279
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