measurements of chromosome aberration at tomato plants
measurements of chromosome aberration at tomato plants
measurements of chromosome aberration at tomato plants
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1,81,61,41,210,80,6D0D1D2D30,40,20% Prophases %Metaphaseswith anomalies with anomalies%A+T withanomaliesOther types <strong>of</strong>anomaliesFig. 3: Types and frequency <strong>of</strong> chromosomal <strong>aberr<strong>at</strong>ion</strong>s observedin root meristem<strong>at</strong>ic cells - genotype L27STable 5Types and frequency <strong>of</strong> chromosomal <strong>aberr<strong>at</strong>ion</strong>s observed in root meristem<strong>at</strong>ic cells – genotype FRANCESCAVariant Total no<strong>of</strong> cells% Prophaseswith anomalies%Metaphaseswith anomalies%A+T withanomaliesOther types<strong>of</strong>anomaliesD0 6021 0,52 0,41 1,96 1,11D1 5478 0,24 0,26 1,32 1,00D2 5965 0,16 0,12 1,02 0,89D3 5418 0,28 0,22 1,35 1,2921,81,61,41,210,80,6D0D1D2D30,40,20% Prophaseswith anomalies%Metaphaseswith anomalies%A+T withanomaliesOther types <strong>of</strong>anomaliesFig. 3: Types and frequency <strong>of</strong> chromosomal <strong>aberr<strong>at</strong>ion</strong>s observedin root meristem<strong>at</strong>ic cells - genotype FrancescaThe main types <strong>of</strong> <strong>aberr<strong>at</strong>ion</strong>s identified <strong>at</strong>regener<strong>at</strong>ed <strong>plants</strong> are: <strong>chromosome</strong> clumping,contraction, stickiness, paling, fragment<strong>at</strong>ion,dissolution, <strong>chromosome</strong> and chrom<strong>at</strong>id bridges, C-mitosis and endoploidy.For all the genotypes tested in thepresent study the highest incidence <strong>of</strong> <strong>aberr<strong>at</strong>ion</strong>s wasobserved in ana-telophases. The most commonabnormalities were ana-telophases with simple ormultiple bridges, expelled or l<strong>at</strong>e <strong>chromosome</strong>s andmultipolar ana-telophases – figure 4. We also detectedabnormalities is metaphases th<strong>at</strong> were abnormallyorganized, with ring <strong>chromosome</strong>s, minutes, expelled<strong>chromosome</strong>s, fragment, etc – figure 5.In a smaller number we detected prophases th<strong>at</strong>presented different types <strong>of</strong> chromosomal <strong>aberr<strong>at</strong>ion</strong>slike l<strong>at</strong>e prophases, with ring <strong>chromosome</strong>s, expelled<strong>chromosome</strong>s etc – figure 6.277