Cornea - ARVO

ARVO 2013 Annual Meeting Abstracts by Scientific Section/Group - CorneaEffects of Silicone Hydrogel Contact Lenses Applied ImmediatelyAfter Sub-Bowman’s KeratomileusisXingwu Zhong, Shaohui Gao. State Key Laboratory ofOphthalmology, Zhongshan Ophthalmic Center, Guangzhou, China.Purpose: To evaluate the effects of silicone hydrogel contact lensesapplied immediately after Sub-bowman keratomileusis.Methods: The study was based on 46 myopic patients (92consecutive eyes) who underwent bilateral Sub-BowmanKeratomileusis (SBK). Each patient wore a PureVision contact lensin one randomly-selected eye and nothing in the fellow eyeimmediately after SBK. The contact lens was removed the next day.Fluorescein staining(FL), tear film break up time(TBUT), schirmer Itest(SIT), central corneal thickness(CCT), ocular surface diseaseindex(OSDI), corneal hysteresis(CH), corneal resistance factor (CRF)and corneal flap-related complications (postoperatively) wereassessed preoperatively and 1 day (except for CH and CRF), 1 week,1 month and 3 months postoperatively.Results: FL and TBUT of treated eye were improved at 1 day and 1week postoperatively in contrast with the controlled, but significantlyaggravations were found at 1 day and 1 week after SBK comparedwith preoperative level. There was no significantly difference in SITbetween treated and controlled eye postoperatively, but both eyesshowed the same reduction contrasted with preoperation except for 1day. CCT of treated eye was thinner than controlled at 1 day aftersurgery and both decreased obviously compared with preoperatively.OSDI of treated eye was alleviated more at 1 day after SBK than thecontrolled, but a significant severity existed at any visit for both incontrast with that before surgery. There were no differences in CHand CRF between treated and controlled eye after surgery, but botheyes showed significantly decrease at any follow-up time. Noobviously differences were found in complications related to thecorneal flap between treated and controlled eye postoperatively.Conclusions: Silicone hydrogel contact lenses applied immediatelyafter SBK can effectively reduce corneal epithelium staining ,increase tear film stability , relieve corneal edema and alleviatediscomfort. However, it can’t shorten the duration of ocular surfacechanges and symptoms revalant to SBK.Commercial Relationships: Xingwu Zhong, None; Shaohui Gao,NoneSupport: National Natural Science Foundation of ChinaGrantNO.81070754 and the Fundamental Research Funds of StateKey Lab of Ophthalmology Grant NO.2010C07.Clinical Trial: under checkProgram Number: 503 Poster Board Number: B0140Presentation Time: 10:30 AM - 12:15 PMHigh levels of sIgA and exudated serum albumin in tears ofcontact lens related Dry Eye patients three months afterdiscontinuation of lens usePiera Versura 1 , Alberto Bavelloni 2 , Chiara Coslovi 1 , WilliamBlalock 3 , Marco Grillini 1 , giuseppe giannaccare 1 , Emilio C.Campos 1 . 1 Ophthalmology Unit, DIMES Department, Alma MaterStudiorum University of Bologna and S.Orsola-Malpighi TeachingHospital, Bologna, Italy; 2 Laboratory of Musculoskeletal CellBiology, Istituto Ortopedico Rizzoli, Bologna, Italy; 3 Institute ofMolecular Genetics, CNR-National Research Council of Italy,Bologna, Italy.Purpose: To evaluate discontinued contact lens (CL) wearers withparticular reference to secretory IgA (sIgA) and exudated serumalbumin in tears as markers of local inflammation.Methods: 45 CL wearers diagnosed as having dry eye (DE)according to DEWS guidelines (DEWS grade 1-3) and 25 matchednormal non-CL wearer volunteers were included in this study.Patients had discontinued the use of various types of CLs (12 RGP,33 soft disposable or frequent replacement CL-average time from lastuse 60±30 days) because of associated discomfort. OSDIquestionnaire score, Schirmer test I, Break Up Time (BUT),Lissamine green vital staining, corneal esthesiometry (Cochet-Bonnet), conjunctival scraping and imprint cytology were performed.Total tear protein content (TP), Lysozyme-C (LYS-C), Lactoferrin(LACTO) and exudated serum albumin (ALB) were evaluated(mg/ml) with the 2100 Bioanalyzer (Agilent Technology, CA, USA,P230 Lab-chip kit). Validation of kDa range for heavy chain IgAbands in each lane was carried out as previously described (Versura Pet al, Mol Vis 2012). The sIgA/LYS-C ratio was calculated as anindex of the increased activity of the IgA-producing cells in thelachrymal gland. Data were statistically evaluated and correlated withwear parameters (Pearson’s r) (significance p