Cornea - ARVO

ARVO 2013 Annual Meeting Abstracts by Scientific Section/Group - Corneapopulation and retrospective nature. Further prospective controlledstudies are needed to validate the findings. In summary, Lipiview®testing can increase recognition of incomplete blinking which canguide treatment of dry eye syndrome.Commercial Relationships: Joanne Shen, NoneProgram Number: 6021 Poster Board Number: A0084Presentation Time: 10:30 AM - 12:15 PMExpression of proinflammatory cytokines in tears of aqueousdeficientdry eyesYan Wang, Jun Xiang, JianJiang Xu. Ophthalmology, Eye and ENTHospital of Fudan University, Shanghai, China.Purpose: To evaluate the expression of different cytokines in tears ofSjögren Syndrome dry eye (SSDE), Non-Sjögren syndrome dry eye(NSSDE) and healthy controls.Methods: Twenty SSDE patients with mean age 47±12.9 years, 20NSSDE patients with mean age 45.2±5.9 years and 20 gender andage matched healthy controls were recruited in this study. Schirmer 1test, tear film break-up time (TBUT), cornea fluorescein staining (FS)were examined. Tear samples were taken from using a 10-ul capillarytube to analyze inflammatory cytokine levels. Expression of IL-1β,TNF-α,IL-6,IL-8,IL-10 and IL-12 were measured by cytometricbead array (BD Biosciences, Pharmingen). Impression cytologyspecimens were obtained and PAS staining was performed toevaluate the goblet cell density.Results: The Schirmer 1 test value, TBUT and FS scores wereobviously worse in NSSDE and SSDE patients comparison with thehealthy controls, specially in the SSDE group. Goblet cell densitywas 1230.24±653cells/mm2 in healthy controls, 706±453cells/mm2in NSSDE group and 340±248cells/mm2 in NSSDE group. Nosignificant difference were found in the expression of IL-6,TNF-α,IL-10,IL-12 in tears of three groups. However a significantly increasedexpression of IL-1β and IL-8 in tears of SSDE group were examined(P