Cornea - ARVO

ARVO 2013 Annual Meeting Abstracts by Scientific Section/Group - CorneaAS-OCT was performed on the first postoperative day using theCirrus HD-OCT (Carl Zeiss Meditec, Dublin, CA). The clear cornealincision length, incision depth, angles of the tri-planar cornealincision, and wound gaping were measured using software calipers.The variability in wound length, depth and angle were calculated andcompared to the programmed software settings. Five architecturalfeatures were used to describe the clear corneal incisions: gaping ofthe wound at the epithelial side, gaping of the wound at theendothelial side, within wound gape, misalignment of the roof andfloor of the incision at the endothelial side and local Descemet’smembrane (DM) detachment. This study is ongoing and will include30 eyes.Results: On analysis of first post-operative day AS-OCT images ofthe initial 3 eyes undergoing femtosecond cataract extraction, 2/3eyes had endothelial side wound gape, 1/3 had epithelial wound gape,2/3 eyes had a within wound gape and 3/3 eyes had a focal DMdetachment. All three eyes had visible 3-plane profile on AS-OCT.The clear corneal incision length was within 100 microns of theintended length. The incision depth was within 8 percent of theintended depth.Conclusions: Our initial results suggest that clear corneal incisionsusing the femtosecond cataract laser were close to the intended sizeand depth. A significant proportion of eyes had marginal and stromalwound gape and focal DM detachment.Commercial Relationships: Surendra Basti, None; Dilraj S.Grewal, NoneSupport: An Unrestricted Grant From Research To PreventBlindness, Inc., New York, New YorkA: slit lamp photo of EBMD B: in-vivo confocal microscopy (IVCM)revealing linear hyperreflective structures corresponding to abnormalepithelial basement membrane extending into the corneal epitheliumC: anterior segment OCT (AS-OCT) revealing hyperreflectivematerial in the posterior epithelium and anterior stromaProgram Number: 564 Poster Board Number: B0201Presentation Time: 10:30 AM - 12:15 PMEpithelial Basement Membrane Dystrophy: A Study with in vivoConfocal Microscopy and High-Resolution Anterior SegmentOptical Coherence TomographyPeter Wu 1 , Dennis E. Cortes 1, 2 , Jennifer Li 1 , Michael Chen 1 , Mark J.Mannis 1 . 1 Ophthalmology & Vision Sciences, University ofCalifornia Davis Eye Center, Sacramento, CA; 2 Ophthalmology,Universidad Catolica de Chile, Santiago, Chile.Purpose: To report the pathological changes of patients withepithelial basement membrane dystrophy (EBMD), the most commonhereditary anterior corneal dystrophy, using in vivo confocalmicroscopy (IVCM) and high-resolution anterior segment opticalcoherence tomography (AS-OCT).Methods: Five patients with EBMD seen in clinical practice wereevaluated with a high-resolution spectral-domain AS-OCT(Heidelberg Engineering, Germany) and IVCM using the HeidelbergRetina Tomograph 3 Rostock Cornea Module (HeidelbergEngineering, Germany).Results: AS-OCT revealed hyperreflective material in the posteriorepithelium and anterior stroma. These findings were correlated withIVCM findings that showed multiple linear and curvilinearhyperreflective structures, corresponding to abnormal epithelialbasement membrane extending into the corneal epithelium.Additionally, IVCM revealed hyperreflective deposits in the anteriorstroma with signs of activation of anterior keratocytes, intraepithelialmicrocysts, and clusters of epithelial cells in the tear film.Conclusions: The acquisition of high-resolution imaging of thecornea with IVCM and AS-OCT provides new insights into themicrostructural characteristics of EBMD and may be usefulmodalities in elucidating the pathogenesis and natural course of thiscorneal dystrophy.A: slit lamp photo of EBMD B: oblique section of in-vivo confocalmicroscopy revealing an area of fibrosis at the sub-epithelial level C:anterior segment OCT (AS-OCT) demonstrating hyperreflectivematerial in the posterior epithelium and anterior stromaCommercial Relationships: Peter Wu, None; Dennis E. Cortes,None; Jennifer Li, None; Michael Chen, None; Mark J. Mannis,NoneProgram Number: 566 Poster Board Number: B0203Presentation Time: 10:30 AM - 12:15 PMStudy Of Ocular Surface impairment in children presentingongoing dry eye with MGD with Meibomian Gland AnalysisDominique Bremond-Gignac 1, 2 , Solange Milazzo 1 . 1 Ophthalmology,St Victor Center, University Hospital of Amiens, Picardie JulesVerne University, Amiens, France; 2 CNRS IRIS UMR8194, Paris VUniversity, Paris, France.Purpose: To evaluate in a retrospective study, the ocular surfaceimpairment in children presenting ongoing MGD with an analysis ofmeibomian glands. Tear film quality is conditionned by meibomiangland production of the lipid layer. Anomaly of this production inchildren can lead to ocular surface impairment and an evaluation ofMeibomian Glands is a key point of the exploration of productionfunction.Methods: Our retrospective study included 12 children (22 eyesevaluated) with a mean age 11yo, range 4 to 17yo, of two groups thatpresented at ocular consultation. Group a, 6 with ongoing MGD,group b 6 control children who had been tested with no ocular surfaceimpairment. All children underwent a Meibomian Gland Analysiswith meibography images with infrared illumination acquired withCobra system. An evaluation with Phoenix software calculated areaof loss of glands. The density of inferior eyelids Meibomian glandshad also been evaluated.Results: In group a, all children presented clinical signs of MGD dueto ocular blepharitis or dry eye. In group b no ocular signs of©2013, Copyright by the Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Go to iovs.org to access the version of record. 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