Cornea - ARVO

ARVO 2013 Annual Meeting Abstracts by Scientific Section/Group - Corneawere seen in the posterior stroma. In LCD, hyperreflective linear andbranching structures with poorly demarcated margins were noted inthe stroma. In PPMD, a variety of vesicular and linear abnormalitiescould be identified at the level of the endothelium. In FD, Descemet'smembrane was thickened with round hyporeflective structures(guttae) at level of the endothelium,with fibrosis and activatedkeratocytes seen in the stroma.Conclusions: AS-OCT and IVCM are non-invasive, high-resolutionimaging modalities useful in the visualization of the cornealmicrostructural changes related to corneal dystrophies. These newimaging technologies are rapidly becoming widely used instrumentsfor both research and patient care, and may be useful in elucidatingthe pathogenesis and natural course of corneal dystrophies.The figure 1 shows full-field OCT images of the cornea from the 3Ddata stack. The upper four images are en face optical slicesdescending through the sample depth: EP epithelium; BM Bowman'smembrane; ST stroma; DM+E Descemet's membrane andendothelium. The lower image is a depth slice through the corneashowing the different layers as labelled above. Scale bars show100μm.Commercial Relationships: Wajdene Ghouali, None; KateGrieve, None; Otman Sandali, None; Elena Basli, None; JulienBullet, None; Laurent Laroche, None; Vincent Borderie, NonePatient with EBMD evaluated with in vivo confocal microscopy(IVCM) and anterior segment OCT (AS-OCT).Program Number: 562 Poster Board Number: B0199Presentation Time: 10:30 AM - 12:15 PMHigh-Resolution Anterior Segment Optical CoherenceTomography and in vivo Confocal Microscopy in the Evaluationof Corneal DystrophiesDennis E. Cortes 1, 2 , Jennifer Li 1 , Michael Chen 1 , Raju Poddar 1 ,Robert J. Zawadzki 1 , John S. Werner 1 , Mark J. Mannis 1 . 1 Departmentof Ophthalmology & Vision Science, UC Davis Eye Center,Sacramento, CA; 2 Department of Ophthalmology, PontificiaUniversidad Católica de Chile, Santiago, Chile.Purpose: To describe the findings observed with high-resolutionanterior segment optical coherence tomography (AS-OCT) and invivo confocal microscopy (IVCM) in patients with cornealdystrophies.Methods: Five patients with common corneal dystrophies seen inclinical practice were evaluated with a high-resolution spectraldomainAS-OCT (Heidelberg Engineering, Germany) and IVCMusing the Heidelberg Retina Tomograph 3 Rostock Cornea Module(Heidelberg Engineering, Germany). A high-speed swept-sourceOCT (SS-OCT) prototype was also used to reconstruct the threedimensionalstructures of the cornea.Results: Patients included in this study had the following cornealdystrophies: epithelial basement membrane dystrophy (EBMD),granular cornea dystrophy (GCD), lattice cornea dystrophy (LCD),posterior polymorphous corneal dystrophy (PPMD) and Fuchs’endothelial dystrophy (FD). AS-OCT and IVCM provided preciseinformation about the pathology in the different layers of the corneain all patients. There was a significant correlation between the twoimaging modalities.In EBMD, we identified an abnormal epithelial basement membraneprotruding into the corneal epithelium and clusters of epithelial cellsin the tear film. In GCD, the majority of opacities were visualized inthe anterior two-thirds of the corneal stroma although rare opacitiesPatient with granular dystrophy evaluated with in vivo confocalmicroscopy (IVCM) and anterior segment OCT (AS-OCT)Commercial Relationships: Dennis E. Cortes, None; Jennifer Li,None; Michael Chen, None; Raju Poddar, None; Robert J.Zawadzki, None; John S. Werner, None; Mark J. Mannis, NoneSupport: NEI 014743, RPBProgram Number: 563 Poster Board Number: B0200Presentation Time: 10:30 AM - 12:15 PMAnalysis Of Early Postoperative Morphologic Features Of ClearCorneal Incisions Created With The Femtosecond CataractLaser Using Anterior Segment Optical Coherence Tomography:Comparison Of Intended Versus Achieved Wound ParametersSurendra Basti, Dilraj S. Grewal. Ophthalmology, NorthwesternUniversity Feinberg School of Medicine, Chicago, IL.Purpose: To analyze the morphology of clear corneal incisions (CCI)performed using a Femtosecond Cataract Laser (Catalys, Optimedica,Santa Clara, CA) in patients undergoing femtosecond laser-assistedcataract surgery using spectral domain anterior segment opticalcoherence tomography (AS-OCT).Methods: Morphology of clear corneal incisions created with theFemtosecond cataract laser was studied with AS-OCT. An intendedtriplanar incision was programmed into the Catalys femtosecond lasersoftware. The first plane was at 90 degree to the corneal surface andextended to 40% corneal depth, the second plane was an angledintrastromal plane and the third plane was at 45 degrees to theposterior corneal surface and reached the second plane at 70% depth.The intended incision width was 2.85 mm and length was 1.8 mm.©2013, Copyright by the Association for Research in Vision and Ophthalmology, Inc., all rights reserved. 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