Cornea - ARVO

ARVO 2013 Annual Meeting Abstracts by Scientific Section/Group - Corneaanalyzed using an EdU proliferation assay, histology, andimmunhistochemical stainings.Results: The FSCS had a light scattering value of log(s) =1.62 anddirect light transmission of 90%. Human stromal cells and HCECsproliferated on the FSCS. HCECs were positive for differentiationmarker CK3/12, several adhesion markers and deposited laminin.Treatment with dispase II and laser generated horizontal tunnelsallowed stromal cells to extend their cytoplasma in between thecreated spaces.Conclusions: This easy obtainable, natural occurring FSCSdemonstrates a reasonable optical clarity, is able to sustain (primary)corneal epithelium and seem to facilitate attachment for stromal cells.These properties highlight its potential as candidate for cornealreconstruction and as alternative to current keratoprostheses. Moreresearch on cellular adhesion and reduction of light scattering will beperformed.Commercial Relationships: T H. van Essen, Aeon Astron EuropeB.V. (F); Sarah J. Sparks, Aeon Astron Europe BV (E); Lisannevan Zijl, Aeon Astron Europe BV (E); Greg Chen, None; Chien C.Lin, None; Horng J. Lai, Aeon Astron Europe BV (E); GregoriusP. Luyten, None; Abdoelwaheb El Ghalbzouri, None; Martine J.Jager, Aeon Astron (F), Agentschap.nl (F)Support: Agentschap.nl (Ministry of Economic Affairs)manual technique, it exceeded 69°C which is higher than bone’sdenaturation temperature.Conclusions: There was no difference in the effectiveness of the 3bonding agents at 13 months. A dynamic stress test will be necessaryto differentiate between the bonding agents. The µ-mill keeps thetemperature below the denaturation temperature and thus is safer thanthe manual technique. These two new observations can improve theMOOKP surgery technique.Acknowledgments: The authors acknowledge the scientificcontributions of Giancarlo Falcinelli and Andres Bernal.Program Number: 3481 Poster Board Number: D0108Presentation Time: 11:00 AM - 12:45 PMIMPROVEMENTS IN THE MODIFIED OSTEO-ODONTOKERATOPROSTHESIS (MOOKP) SURGERY TECHNIQUEMariela C. Aguilar 1 , Yoh Sawatari 2 , Alex Gonzalez 1 , William Lee 1 ,Cornelis J. Rowaan 1 , Deepa Sathiah 2 , Darlene Miller 3 , Victor L.Perez 3 , Eduardo C. Alfonso 3 , Jean-Marie A. Parel 1 . 1 OphthalmicBiophysics Center, Department of Ophthalmology, Bascom PalmerEye Institute, University of Miami Miller School of Medicine,Miami, FL; 2 Oral Maxillofacial Division, Department of Surgery,University of Miami Miller School of Medicine, Miami, FL;3 Department of Ophthalmology, Bascom Palmer Eye Institute,University of Miami Miller School of Medicine, Miami, FL.Purpose: To assess the long-term effectiveness of 3 bonding agentsthat are periodically subjected to high static pressures on the opticalcylinder face mimicking traumatic ocular incidents, and to develop anautoclavable µ-milling device designed to contour and drill theMOOKP lamina in an efficient and precise manner.Methods: An instrument was fabricated to stabilize the lamina andapply direct pressure to the cemented optical cylinder. The strengthsof cyanoacrylate adhesive (Histoacryl), a 2-part bone cement(Stryker), and the dental acrylic (Jet) were compared. 4 laminas peragent preserved at 36°C in 30cc of BSS with antibiotic (gatifloxacin2µg/cc) and antifungal (amphotericin B 0.2mg/cc) for >12 monthswere subjected monthly to static forces ranging from 200g, 500g, and1kg (1kg force = 200mmHg IOP equivalent). The force range wasexpanded to include 2kg and 3kg at month 13. An autoclavable µ-mill was developed to adapt a commercially available Stryker drill;using extracted canines, laminas were fashioned while monitoringinternal temperatures via two thermocouples.Results: All 12 laminas resisted the applied static forcescorresponding to pressures of 2, 5 and 10kg/cm 2 and the 30kg/cm 2applied at month 13. The µ-mill performance was tested on resectedcadaveric lamina specimens. Planar surfaces are better than 50µm,perpendicularity is better than 1 degree; drilling departure fromovality is less than 10µm assuring excellent fit with the opticalcylinder. The µ-mill was shown to be as efficacious as the manualtechnique, as it took less than 10 min to create a lamina. Moreover,the canine’s canal temperature never exceeded 38°C whereas with theInstrument fabricated to stabilize the lamina and apply direct pressureto the cemented optical cylinder.Autoclavable µ-milling device.Commercial Relationships: Mariela C. Aguilar, None; YohSawatari, University of MIami (P); Alex Gonzalez, None; WilliamLee, None; Cornelis J. Rowaan, None; Deepa Sathiah, None;Darlene Miller, None; Victor L. Perez, Alcon (C), Bausch & Lomb(C), Genentech (C), Cleveland Clinic Foundation (P), Alcon (F),Alcon (R); Eduardo C. Alfonso, Bio Tissue (C); Jean-Marie A.Parel, CROMA (F), InnFocus (F), Abeamed (F), University ofMiami (P)Support: DOD Grant DAMD-W81XWH-09-1-0675; NIHR01EY018624-04, NIH Center Grant P30EY14801, Florida LionsEye Bank, RPB Physician Scientist Grant (VLP), Henri and FloreLesieur Foundation (JMP).371 Refractive SurgeryTuesday, May 07, 2013 2:45 PM-4:30 PMTCC 303 Paper SessionProgram #/Board # Range: 3711-3717Organizing Section: CorneaProgram Number: 3711Presentation Time: 2:45 PM - 3:00 PM©2013, Copyright by the Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Go to iovs.org to access the version of record. For permissionto reproduce any abstract, contact the ARVO Office at arvo@arvo.org.