Cornea - ARVO

ARVO 2013 Annual Meeting Abstracts by Scientific Section/Group - Corneameasuring the compressive moduli. Resistance of the corneas toenzymatic degradation was evaluated by exposure to clostridiumcollagenase solution.Results: Porcine corneas coated with PDA (Group C) showedsignificantly longer degradation time (>91h) than both control(5.0±0.7 h) and PEG treated groups (26±2.7 h). Mechanical strengthtesting revealed a difference between both PEG and PDA treatedgroups compared to the control group.Conclusions: PEG- and PDA-modified corneas showed a markedlyincreased resistance to enzymatic degradation. The creation of amussel inspired PDA coating on corneas may increase the strengthand effectiveness of keratoprosthesis carrier tissue. In addition, PDAmodifiedcorneas turn black which would reduce glare - a majorproblem in many keratoprosthesis cases (aniridia, etc.). Further invivo studies are needed to assess the viability and safety of thesemodified corneas.PMMA structures. Image projections created via confocalmicroscopy show that the depth of cell growth in Gas FoamedPHEMA-PMMA was greater than that observed on Salt PHEMA-PMMA. Day 7 image projections of Gas Foamed PHEMA-PMMAshow viable cells at depths of approximately 100 µm below thesurface on which cells were seeded. For Salt PHEMA-PMMA, thedepth of cell growth is less pronounced at day 7; however, cellproliferation data confirms that Salt PHEMA-PMMA structures arecytocompatible. SEM and μCT data indicate that both structures havea high density of pores. Among the two structures, Gas FoamedPHEMA-PMMA appeared to have the higher pore interconnectivity.For Gas Foamed PHEMA-PMMA, elastic modulus (E) and ultimatetensile strength (UTS) are 4081 ± 808 kPa and 263 ± 66 kPa,respectively. For Salt PHEMA-PMMA, E and UTS are 678 ± 72 kPaand 125 ± 25 kPa, respectively.Conclusions: Pore architecture, mechanical stability, andcytocompatibility are vital design parameters for KPros. PorousPHEMA-PMMA is cytocompatible. Increased pore interconnectivityappears to allow greater cell growth into the body of porousPHEMA-PMMA structures. The polymers appear to be strongenough to be sutured and to maintain their structures under ocularforces as host tissue integrates. KPros made with porous PHEMA-PMMA may provide additional options for patients for whom donorcorneas are inappropriate or inaccessible.Polydopamine modified cornea as carrier for the BostonKeratoprosthesis.Commercial Relationships: Sara Bozorg, None; Kyung Jae Jeong,None; Samer N. Arafat, None; Daniel S. Kohane, None; Claes H.Dohlman, NoneProgram Number: 3479 Poster Board Number: D0106Presentation Time: 11:00 AM - 12:45 PMDesigning a Novel Porous Keratoprosthesis to Promote CorneaCell IngrowthAmelia L. Zellander 1 , Richard A. Gemeinhart 2 , Behrad Milani 3 , AliR. Djalilian 3 , Mohsen Makhsous 4 , Michael Cho 1 . 1 Bioengineering,University of Illinois at Chicago, Chicago, IL; 2 BiopharmaceuticalSciences, University of Illinois at Chicago, Chicago, IL;3 Ophthalmology and Visual Sciences, University of Illinois atChicago, Chicago, IL; 4 Physical Medicine and Human MovementSciences, Northwestern University, Chicago, IL.Purpose: Limited donor cornea supplies and cornea transplantrejection necessitate the development of safe and effectivekeratoprostheses (KPros). This study evaluates cell growth into novelporous polymers that could be used in a corneal replacement device.Methods: Porous Salt PHEMA-PMMA was composed of 10% v/vmethyl methacrylate (MMA), 45% v/v 2-hydroxyethyl methacrylate(HEMA), and 0.07 M sodium chloride. Porous Gas FoamedPHEMA-PMMA was composed of 20% v/v MMA and 40% v/vHEMA. Prior to the introduction of human corneal fibroblasts(HCFs), polymer samples were coated with collagen type I. Cellproliferation and viability were assessed using AlamarBlue and LiveDead Cell Viability assays (Invitrogen), respectively. The structuresof the porous PHEMA-PMMA samples were evaluated usingscanning electron microscopy (SEM) and micro-computedtomography (μCT). Mechanical properties were evaluated in tension.Results: A high level of cell viability was observed on PHEMA-SEM ImagesCommercial Relationships: Amelia L. Zellander, Tebios (F);Richard A. Gemeinhart, None; Behrad Milani, None; Ali R.Djalilian, None; Mohsen Makhsous, None; Michael Cho, NoneSupport: Office of Naval Research N00014-06-1-0100Program Number: 3480 Poster Board Number: D0107Presentation Time: 11:00 AM - 12:45 PMA fish scale-derived scaffold for corneal reconstructionT H. van Essen 1 , Sarah J. Sparks 2 , Lisanne van Zijl 2 , Greg Chen 3 ,Chien C. Lin 3 , Horng J. Lai 3 , Gregorius P. Luyten 1 , Abdoelwaheb ElGhalbzouri 2 , Martine J. Jager 1 . 1 Ophthalmology, Leiden UnivMedical Center, Leiden, Netherlands; 2 Dermatology, Leiden UnivMedical Center, Leiden, Netherlands; 3 Research, Aeon AstronEurope B.V., Leiden, Netherlands.Purpose: A natural occurring, easy obtainable fish scale-derivedcollagen scaffold (FSCS) has been developed for reconstructing thecornea. This FSCS could form a cheap and simple alternative tocurrent keratoprostheses. We assessed in vitro whether the FSCS canbe repopulated by epithelium and fibroblasts. In addition, weevaluated cell adhesion of the FSCS and measured its opticalproperties.Methods: The light scattering and transmission values of the FSCS, adecalcified and decellularized layered build collagen type Iextracellular matrix, were measured. In addition, human cornealepithelial cells (HCECs) and human corneal stromal cells were coculturedwith the FSCS. Dispase and laser treatments were used tooptimize entrance for stromal cells into the FSCS. The cytotoxicity ofFSCS was assessed using an MTT assay. The effect of FSCS oncellular repopulation, morphology, phenotype, and adhesion were©2013, Copyright by the Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Go to iovs.org to access the version of record. For permissionto reproduce any abstract, contact the ARVO Office at arvo@arvo.org.