Cornea - ARVO

ARVO 2013 Annual Meeting Abstracts by Scientific Section/Group - Corneamelting. Early identification of corneal/scleral thinning and meltingin Boston type I KPro patients allows for early intervention and maylead to improved outcomes.Commercial Relationships: David Sola-Del Valle, None;Bernardo M. Cavalcanti, None; Andrea Cruzat, None; Claes H.Dohlman, None; Pedram Hamrah, NoneSupport: Keratoprosthesis Manufacturing Fund, NIH K08-EY020575, Research to Prevent Blindness Career DevelopmentAward, Falk Medical Research TrustProgram Number: 3467 Poster Board Number: D0094Presentation Time: 11:00 AM - 12:45 PMScanning Laser Ophthalmoscope Microperimetry through theBoston KeratoprosthesisRony R. Sayegh, Claes H. Dohlman, Mary Lou Jackson.Ophthalmology, Massachusetts Eye & Ear Infirmary, Boston, MA.Purpose: Scanning laser ophthalmoscope (SLO) microperimetry isuseful in accurately mapping macular function. We explore thefeasibility and potential use of this method in eyes implanted with aBoston keratoprosthesis.Methods: Retrospective review of eyes with a type I Bostonkeratoprosthesis tested with OPKO SLO microperimetry. Bestcorrectedvisual acuity, contrast sensitivity and complete slit-lampand posterior segment examination findings were noted. Visual fieldand ocular coherence tomography results were collected whenavailable.Results: Five eyes were included in this study. Mean age was 66years (range: 54 to 82). Mean LogMAR best-corrected visual acuitywas 0.76 (range: 0.30 to 1.20). Contrast sensitivity was decreased inall eyes at a mean of 0.90 log units (range: 0.45 to 1.5). SLOmicroperimetry could be performed on all 5 eyes. Accurate functionalmapping of the macula and preferred retinal locus was obtained in allcases and was not affected by media opacities or decreased contrastsensitivity. The results correlated well with the findings on Goldmannperimetry and OCT in cases in which these were available.Conclusions: SLO microperimetry through the Bostonkeratoprosthesis is possible. It is a good adjunct to standard perimetrywith involvement of central fixation for accurate macular mapping. Itmay also allow better assessment of macular pathology unveiled byclearing of the visual axis with a keratoprosthesis.Commercial Relationships: Rony R. Sayegh, None; Claes H.Dohlman, None; Mary Lou Jackson, Nonehistologic appearance (H&E staining) and presence of inflammation(immunolocalization of CD45 positive cells) in rabbit corneal tissues50 days after an intralamellar implant of the modified Ti. Surfacecharacterization of the modified Ti included assessment by scanningelectron microscopy (SEM), X-ray diffraction crystallography(XRD), Fourier transform infrared spectroscopy (FTIR) andchemical/color stability was determined after exposure to 70%alcohol for three months.Results: Blue and brown coloration of Ti was achieved byanodization at 33 and 13.4 volts, respectively with a current supply of3 Amps, (Figure 1). The in vitro biocompatibility assays showed nosignificant differences in cell proliferation, cytotoxicity or migrationbetween HCLE cells co-cultured with surface modified or nonmodifiedTi (p> 0.745 for all group comparisons. Analysis of cornealtissues that had harbored the Ti implants showed normal cellappearance, and lack of CD45 or TUNEL positive cells. SEMshowed the presence of a nano porous surface and similarcrystallographic prints were observed with XRD and FTIR for thenon-coated and the modified Ti, (Figure 2). Three months exposureto alcohol had no effect on the color or color homogeneity of theoxidized surface.Conclusions: Ti backplate coloring was achieved by altering thevoltage of the electrochemical anodization and controlling thethickness of the Ti oxidation. In vitro and in vivo results suggest thatthe modified Ti is equally biocompatible and as safe as the standardnon-coated Ti. The color modification of the BKpro may improve thecosmesis and acceptance of the KPro by patients.*Supported by The Boston Keratoprosthesis Fund at theMassachusetts Eye and Ear Infirmary.Program Number: 3468 Poster Board Number: D0095Presentation Time: 11:00 AM - 12:45 PMAssessment of Titanium Modification for Coloring the Backplateof Boston KeratoprosthesisEleftherios I. Paschalis 1, 2 , James Chodosh 1 , Sandra J. Spurr-Michaud 2 , Andrea Cruzat 1 , Allyson Tauber 1 , Irmgard Behlau 1 , IleneK. Gipson 2 , Claes H. Dohlman 1 . 1 Ophthalmology, Massachusetts Eyeand Ear Infirmary - Harvard Medical School, Boston, MA;2 Ophthalmology, Schepens Eye Research Institute - Harvard MedicalSchool, Boston, MA.Purpose: Recent use of a titanium (Ti) backplate has improved thedesign and biocompatibility of the Boston Keratoprosthesis (BKPro).Titanium’s shiny metallic appearance, however, makes itcosmetically less favorable. The purpose of this study was to developand test a coloring, surface modification of Ti.Methods: Ti coloring was achieved electrochemically by controlledanodization of the Ti surface, which creates an oxide film on the Ti.Biocompatibility was assessed in vitro by co-culture of the modifiedTi with human corneal limbal epithelial cells (HCLE) followed byassays of cell proliferation, cytotoxicity and migration, and in vivo byCommercial Relationships: Eleftherios I. Paschalis, None; JamesChodosh, Alcon (C), Allergan (C), 3-V Biosciences (C), Novabay(C); Sandra J. Spurr-Michaud, None; Andrea Cruzat, None;Allyson Tauber, None; Irmgard Behlau, None; Ilene K. Gipson,None; Claes H. Dohlman, NoneProgram Number: 3469 Poster Board Number: D0096Presentation Time: 11:00 AM - 12:45 PM©2013, Copyright by the Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Go to iovs.org to access the version of record. For permissionto reproduce any abstract, contact the ARVO Office at arvo@arvo.org.