Cornea - ARVO

ARVO 2013 Annual Meeting Abstracts by Scientific Section/Group - Corneaserially diluted 10-fold in PBS and 10-μl aliquots of each dilutionwere spotted on LB agar plates. The plates were incubated at 37degrees C for 24 hours and the CFU were counted. For confocal laserscanning microscopy (CLSM), we used the S. aureus strain AH1333which carries the gene that encodes the green fluorescent protein.Results: Colony forming unit assays showed total inhibition,representing over 6 logs of Staphylococcus aureus killing on organoseleniumpolymerized hydrogels. Confocal laser scanningmicroscopy confirmed these results.Conclusions: The organo-selenium hydrogel polymer successfullyblocked the formation of a bacterial biofilm on the polymer byStaphylococcus aureus in vitro.Commercial Relationships: Phat L. Tran, None; Abdul Hamood,None; Daniel Webster, None; Courtney Jarvis, None; Robert E.Hanes, Selenium, Ltd. (F), Selenium, Ltd. (I), Selenium, Ltd. (E),Selenium, Ltd. (P); Ted W. Reid, Selenium Ltd. (I), Selenium Ltd.(P)Program Number: 515 Poster Board Number: B0152Presentation Time: 10:30 AM - 12:15 PMContact Lens Disinfecting Solution vs. Blister Pack: a SubjectiveEvaluationScott Schatz 1 , Balamurali Vasudevan 2 , Sara N. Gaib 3 , KimbalCooper 4 . 1 Appalachian College of Optometry, Grundy, VA; 2 Collegeof Optometry, Midwestern University, Glendale, AZ; 3 College ofOptometry, Midwestern University, Glendale, AZ; 4 College of HealthSciences, Midwestern University, Glendale, AZ.Purpose: To study subjectively the effect of contact lens disinfectingsolution on the ocular surface in the presence of either a hydrogel or asilicone hydrogel soft contact lens.Methods: Twenty young adult subjects were examined for this studyover 2 visits. Subjects were fit (randomized) with either a hydrogellens or silicone hydrogel lens in either eye. The lenses were presoakedovernight in either Puremoist or Revitalens contact lensdisinfecting solution or were obtained from the blister pack in arandomized process. Subjective feedback on discomfort, burning,dryness and irritation on a scale of 1 to 5 was obtained at baseline,prior to lens insertion, and after 8 hours of wear. Chi-square analysiswas performed.Results: There was a statistically significant increase in dryness afterlens insertion for Proclear and Purevision lenses taken from theblister pack , relative to baseline (p = 0.01). Following 8 hours of lenswear, there was a statistically significant increase in burningsensation relative to baseline for all Proclear lenses. This held truewhether they were obtained from the blister pack or pre-soaked inRevitalens MPDS or Puremoist MPDS (p=0.01).Conclusions: Subjectively, lenses from the blister pack producedmore subjective symptoms of dryness upon initial insertion thanthose pre-soaked in either solution. Proclear lenses produced moresubjective symptoms of burning after 8 hours of wear relative tobaseline irrespective of whether they were obtained from the blisterpack, or pre-soaked in either solution.Commercial Relationships: Scott Schatz, Abbot Medical OpticsInc (F); Balamurali Vasudevan, None; Sara N. Gaib,Bausch+Lomb (C), Alcon (R), Vistakon (R), Coopervision (R);Kimbal Cooper, NoneSupport: Study was supported by a grant from Abbott MedicalOpticsProgram Number: 516 Poster Board Number: B0153Presentation Time: 10:30 AM - 12:15 PMAntimicrobial Efficacy of New Investigational MultipurposeDisinfecting Solution and Comparison to CommerciallyAvailable Multipurpose Disinfecting SolutionsMarina Milenkovic, Nancy Brady, Anthony Lam. Corneal R&DMicrobiology, Abbott Medical Optics, Santa Ana, CA.Purpose: To evaluate antimicrobial efficacy of investigationalmultipurpose formulation against bacteria, fungi and Acanthamoebaspp. and compare it to commercially available multipurposedisinfecting solutions (MPS). Study was done according to ISO14729:2001/A.2010 standard.Methods: The multipurpose disinfecting solutions studied were -Investigational MPS-1: polyhexamethylene biguanide (PHMB) +poloxamer (PLX) and currently marketed Japan products MPS-2:polyquaternium (PQ1) + tetronic 1304, MPS-3: PHMB + poloxamine(PLA) and MPS-4: PHMB +PLX.Test organisms were: Staphylococcus aureus (ATCC 6538),Pseudomonas aeruginosa (ATCC 9027), Serratia marcescens (ATCC13880), Candida albicans (ATCC 10231), Fusarium solani (ATCC36031) and Gram-negative clinical isolates. Test solutions wereevaluated at the minimum recommended disinfection time of 4 hours.Disinfectant efficacy of MPS was also evaluated againstAcanthamoeba trophozoites.Results: After 4 hours exposure, Investigational MPS-1 and MPS-3showed >3 log kill of ISO bacteria and fungi. MPS-2 and MPS-4failed to meet stand-alone criteria for ISO bacteria and fungi. MPS-1,MPS-3 and MPS-4 showed >3 log kill against clinical isolates, whileMPS-2 showed 3 logreduction for Acanthamoeba trophozoites, while MPS-4 achieved