Cornea - ARVO

More documents

Recommendations

Info

ARVO 2013 Annual Meeting Abstracts by Scientific Section/Group - Corneadeposition using radiochemical analysis.Methods: Thirteen model lens materials were created based oncombinations of dimethylacrylamide (DMAA), hydroxyethylmethacrylate (HEMA) andmethacryloxypropyltris(trimethylsiloxy)silane (TRIS). The materialswere prepared with and without the incorporation of a hydrophilicnovel substance such as hyaluronic acid, alginate, and a siliconesurfactant. The model materials were hydrated and advancing contactangles were measured using the sessile drop technique. All materialswere incubated in a complex artificial tear solution (ATS) containingprotein, lipid, mucin and a trace amount of either 14 C-CH or 14 C-PCfor 3 and 14 days. All materials were then extracted, processed andmasses of deposition were quantified (ng/disc) using standardcalibration curves.Results: For the HEMA-based materials tested, only the materialsthat incorporated the silicone surfactant and alginate showed astatistically significant increase in wettability (p
ARVO 2013 Annual Meeting Abstracts by Scientific Section/Group - Corneaserially diluted 10-fold in PBS and 10-μl aliquots of each dilutionwere spotted on LB agar plates. The plates were incubated at 37degrees C for 24 hours and the CFU were counted. For confocal laserscanning microscopy (CLSM), we used the S. aureus strain AH1333which carries the gene that encodes the green fluorescent protein.Results: Colony forming unit assays showed total inhibition,representing over 6 logs of Staphylococcus aureus killing on organoseleniumpolymerized hydrogels. Confocal laser scanningmicroscopy confirmed these results.Conclusions: The organo-selenium hydrogel polymer successfullyblocked the formation of a bacterial biofilm on the polymer byStaphylococcus aureus in vitro.Commercial Relationships: Phat L. Tran, None; Abdul Hamood,None; Daniel Webster, None; Courtney Jarvis, None; Robert E.Hanes, Selenium, Ltd. (F), Selenium, Ltd. (I), Selenium, Ltd. (E),Selenium, Ltd. (P); Ted W. Reid, Selenium Ltd. (I), Selenium Ltd.(P)Program Number: 515 Poster Board Number: B0152Presentation Time: 10:30 AM - 12:15 PMContact Lens Disinfecting Solution vs. Blister Pack: a SubjectiveEvaluationScott Schatz 1 , Balamurali Vasudevan 2 , Sara N. Gaib 3 , KimbalCooper 4 . 1 Appalachian College of Optometry, Grundy, VA; 2 Collegeof Optometry, Midwestern University, Glendale, AZ; 3 College ofOptometry, Midwestern University, Glendale, AZ; 4 College of HealthSciences, Midwestern University, Glendale, AZ.Purpose: To study subjectively the effect of contact lens disinfectingsolution on the ocular surface in the presence of either a hydrogel or asilicone hydrogel soft contact lens.Methods: Twenty young adult subjects were examined for this studyover 2 visits. Subjects were fit (randomized) with either a hydrogellens or silicone hydrogel lens in either eye. The lenses were presoakedovernight in either Puremoist or Revitalens contact lensdisinfecting solution or were obtained from the blister pack in arandomized process. Subjective feedback on discomfort, burning,dryness and irritation on a scale of 1 to 5 was obtained at baseline,prior to lens insertion, and after 8 hours of wear. Chi-square analysiswas performed.Results: There was a statistically significant increase in dryness afterlens insertion for Proclear and Purevision lenses taken from theblister pack , relative to baseline (p = 0.01). Following 8 hours of lenswear, there was a statistically significant increase in burningsensation relative to baseline for all Proclear lenses. This held truewhether they were obtained from the blister pack or pre-soaked inRevitalens MPDS or Puremoist MPDS (p=0.01).Conclusions: Subjectively, lenses from the blister pack producedmore subjective symptoms of dryness upon initial insertion thanthose pre-soaked in either solution. Proclear lenses produced moresubjective symptoms of burning after 8 hours of wear relative tobaseline irrespective of whether they were obtained from the blisterpack, or pre-soaked in either solution.Commercial Relationships: Scott Schatz, Abbot Medical OpticsInc (F); Balamurali Vasudevan, None; Sara N. Gaib,Bausch+Lomb (C), Alcon (R), Vistakon (R), Coopervision (R);Kimbal Cooper, NoneSupport: Study was supported by a grant from Abbott MedicalOpticsProgram Number: 516 Poster Board Number: B0153Presentation Time: 10:30 AM - 12:15 PMAntimicrobial Efficacy of New Investigational MultipurposeDisinfecting Solution and Comparison to CommerciallyAvailable Multipurpose Disinfecting SolutionsMarina Milenkovic, Nancy Brady, Anthony Lam. Corneal R&DMicrobiology, Abbott Medical Optics, Santa Ana, CA.Purpose: To evaluate antimicrobial efficacy of investigationalmultipurpose formulation against bacteria, fungi and Acanthamoebaspp. and compare it to commercially available multipurposedisinfecting solutions (MPS). Study was done according to ISO14729:2001/A.2010 standard.Methods: The multipurpose disinfecting solutions studied were -Investigational MPS-1: polyhexamethylene biguanide (PHMB) +poloxamer (PLX) and currently marketed Japan products MPS-2:polyquaternium (PQ1) + tetronic 1304, MPS-3: PHMB + poloxamine(PLA) and MPS-4: PHMB +PLX.Test organisms were: Staphylococcus aureus (ATCC 6538),Pseudomonas aeruginosa (ATCC 9027), Serratia marcescens (ATCC13880), Candida albicans (ATCC 10231), Fusarium solani (ATCC36031) and Gram-negative clinical isolates. Test solutions wereevaluated at the minimum recommended disinfection time of 4 hours.Disinfectant efficacy of MPS was also evaluated againstAcanthamoeba trophozoites.Results: After 4 hours exposure, Investigational MPS-1 and MPS-3showed >3 log kill of ISO bacteria and fungi. MPS-2 and MPS-4failed to meet stand-alone criteria for ISO bacteria and fungi. MPS-1,MPS-3 and MPS-4 showed >3 log kill against clinical isolates, whileMPS-2 showed 3 logreduction for Acanthamoeba trophozoites, while MPS-4 achieved
Page 2 and 3: ARVO 2013 Annual Meeting Abstracts
Page 10: ARVO 2013 Annual Meeting Abstracts
Page 62 and 63:
ARVO 2013 Annual Meeting Abstracts
Page 64 and 65:
Page 66 and 67:
Page 68 and 69:
Page 70 and 71:
Page 72 and 73:
Page 74 and 75:
Page 76 and 77:
Page 78 and 79:
Page 80 and 81:
Page 82 and 83:
Page 84 and 85:
Page 86 and 87:
Page 88 and 89:
Page 90 and 91:
Page 92 and 93:
Page 94 and 95:
Page 96 and 97:
Page 98 and 99:
Page 100 and 101:
Page 102 and 103:
Page 104 and 105:
Page 106 and 107:
Page 108 and 109:
Page 110 and 111:
Page 112 and 113:
Page 114 and 115:
Page 116 and 117:
Page 118 and 119:
Page 120 and 121:
Page 122 and 123:
Page 124 and 125:
Page 126 and 127:
Page 128 and 129:
Page 130 and 131:
Page 132 and 133:
Page 134 and 135:
Page 136 and 137:
Page 138 and 139:
Page 140 and 141:
Page 142 and 143:
Page 144 and 145:
Page 146 and 147:
Page 148 and 149:
Page 150 and 151:
Page 152 and 153:
Page 154 and 155:
Page 156 and 157:
Page 158 and 159:
Page 160 and 161:
Page 162 and 163:
Page 164 and 165:
Page 166 and 167:
Page 168 and 169:
Page 170 and 171:
Page 172 and 173:
Page 174 and 175:
Page 176 and 177:
Page 178 and 179:
Page 180 and 181:
Page 182 and 183:
Page 184 and 185:
Page 186 and 187:
Page 188 and 189:
Page 190 and 191:
Page 192 and 193:
Page 194 and 195:
Page 196 and 197:
Page 198 and 199:
Page 200 and 201:
Page 202 and 203:
Page 204 and 205:
Page 206 and 207:
Page 208 and 209:
Page 210 and 211:
Page 212 and 213:
Page 214 and 215:
Page 216 and 217:
Page 218 and 219:
Page 220 and 221:
Page 222 and 223:
Page 224 and 225:
Page 226 and 227:
Page 228 and 229:
Page 230 and 231:
Page 232 and 233:
Page 234 and 235:
Page 236 and 237:
Page 238 and 239:
Page 240 and 241:
Page 242 and 243:
Page 244 and 245:
Page 246 and 247:
Page 248 and 249:
Page 250 and 251:
Page 252 and 253:
Page 254 and 255:
Page 256 and 257:
Page 258 and 259:
Page 260 and 261:
Page 262 and 263:
Page 264 and 265:
Page 266 and 267:
Page 268 and 269:
Page 270 and 271:
Page 272 and 273:
Page 274 and 275:
Page 276 and 277:
Page 278 and 279:
Page 280 and 281:
Page 282 and 283:
Page 284 and 285:
Page 286 and 287:
Page 288 and 289:
Page 290 and 291:
Page 292 and 293:
Page 294 and 295:
Page 296 and 297:
Page 298 and 299:
Page 300 and 301:
Page 302 and 303:
Page 304 and 305:
Page 306 and 307:
Page 308 and 309:
Page 310 and 311:
Page 312 and 313:
Page 314 and 315:
Page 316 and 317:
Page 318 and 319:
Page 320 and 321:
Page 322 and 323:
Page 324 and 325:
Page 326 and 327:
Page 328 and 329:
Page 330 and 331:
Page 332 and 333:
Page 334 and 335:
Page 336 and 337:
Page 338 and 339:
Page 340 and 341:
Page 342 and 343:
Page 344 and 345:
Page 346 and 347:
Page 348 and 349:
Page 350 and 351:
Page 352 and 353:
Page 354 and 355:
Page 356 and 357:
Page 358 and 359:
Page 360 and 361:
Page 362 and 363:
Page 364 and 365:
Page 366 and 367:
Page 368 and 369:
Page 370 and 371:
Page 372 and 373:
Page 374 and 375:
Page 376 and 377:
Page 378 and 379:
Page 380 and 381:
Page 382 and 383:
show all

Cornea - ARVO

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?