Shinwa GC Capillary Application - Winlab.com.au

Gas ChromatographyCapillary ColumnApplication Data

ULBON SeriesThe capillary column performs very higher separation than the packed column because of the smalldiffusion and good permeability of the sample. The fused silica capillary column coated polyimide resin onthe outside of tubing was developed in around 1980. The inert surface and flexibility of fused silica tubinglead to high performance separation and easy handling. To satisfy your analytical requirement, we arepreparing various columns including the chemical bonding (HR) and wall coated types (WCOT).Products List of Capillary columnHR (Chemical Bond)WCOT (Wall Coated Open Tubular)Fused SilicaInactive ProcessedStainless Steel TubeFused SilicaStainless SteelULBON HR-1GeneralPurposeTypeULBON HR-52ULBON HR-17ULBON HR-1701ULBON HR-20MULBON HR-SS-10ULBON HR-TGC1ULBON ALPhenULBON SPX-1ULBON HR-Thermon-3000BULBON DMN 267SpecialTypeULBON HR-Thermon-600TULBON HR-Thermon-HGULBON PLCULBON Xylene MasterULBON Advance-DSULBON FFAP“ULBON” is a generic name of the Shinwa capillary columns.“ULBON HR” is a generic name of the Shinwa capillary columns with chemical bonding layer.ColumnTypeFeatureMaterialULBONHR SeriesThe chemical bond layerULBON HR series are capillary columnschemically bonded between silicasurface and stationary phase.Fused silica covered withpolyimide resinInactive processed stainlesssteelULBONWCOT SeriesWall Coated (Open Tubular)Capillary column coated with stationaryphase on the inner wall.Fused silica covered withpolyimide resinStainless steelULBON HR SeriesWe have ten types of stationary phases for general analysis and each analytical purpose, as an ULBON HRseries. We also stock the columns with various lengths, inner diameter and the film thickness of stationaryphase for your requirement. Characteristics1. The inert inner surface of the column results in high quantitative analysis.The adsorption point of the inner surface of the column has been completely removed by a rigidtreatment. Therefore, free acid and free amine which are easily adsorbed are eluted without interactionwith the active surface.2. No exfoliation and bleeding of stationary phase lead to high sensitive analysis .In HR series column, the rigid chemical bonding is treated on the surface, therefore there is almost noexfoliation and bleeding of stationary phase. No bleeding from the column results in the high sensitiveanalysis.3. The maximum temperature is higher than that of ordinary WCOT column.The maximum temperature of HR-20M (correspondence to PEG-20M) is 240 and HR-siliconeseries are 330 (except HR-1701 and HR-17). The maximum temperature of chemical bond typecolumn rises about 70 compared with the WCOT type.2

ULBON WCOT SeriesFor Alkyl Phenol Analysis ULBON ALPhenULBON SeriesIsomers of the alkyl phenol compounds such as cresol, xylenes and ethyl phenols which are known todifficult substances to analyze can be completely separated with ULBON ALPhen.All isomers of xylenol (six isomers) can be separated on the ULBON ALPhen, especially 2,4-Xylenoland 2,5-Xylenol are resoluted completely.The separation of the isomers of the cresol and the ethyl phenol is excellent.ALPhen can offer you the best analysis of the alkyl phenol mentioned above.6. For 2,6-, and 2,7-Dimethylnaphthalene Analysis ULBON DMN267Dimethylnaphthalene have ten isomers. Of ten isomers, the chemical and physical characteristics of 2,6-DMN and 2,7-DMN is very similar. The two isomers can be separated on the ULBON DMN267 (WCOTtype) .ULBON DMN267 has the following characteristics.Maximum operation temperature is 160 . ULBON DMN267 is stable column.The column life is long.7. For Dimethylnaphthalene Isomers Analysis ULBON PLCRecently, high purity 2,6-Dimethylnaphthalene (DMN) has attracted attention as a high functional rawmaterial of heat-resistance and the high strength resin. There are ten isomers of DMN. Because of thesimilar physical and chemical characteristics of DMN, the separation of 2,6-DMN and 2,7-DMM isespecially difficult in gas chromatography. ULBON PLC is a capillary column to separate all isomers ofDMN completely.The stationary phase of ULBON PLC is a liquid crystal phase developed by our technical group. Theoperation temperature range (the appearance of liquid crystal state) as the stationary phase is 150-160 ,and a fine separation of 2,7-DMN, 1,3-DMN and 2,6-DMN can be obtained by the proper control of the oventemperature.To maintain good column performance, please take care of the following points.Please analyze efficiently to prevent the deteriorate of stationary phase.hen the analysis is temporarily interrupted, please adjust the temperature of the column oven to 50 or less or stop the apparatus without stopping the career gas.As for the career gas, nitrogen is more excellent than helium in the point of the separation and thedetection sensitivity.8. For Xylene Isomers Analysis ULBON Xylene MasterXylene Master shows excellent separation because of the about 1.1 relative retention valuefor the isomer of xylene.The large separation capacity of Xylene Master capillary column and the inactive surface offused silica used as a base material lead to perform the trace analysis of several ppm. The maximum operation temperature is 160 . The heat-resistance of Xylene Master ishigher than that of the stationary phase of phthalic acid ester for general analysis.Therefore, the analysis of the alkyl benzene isomer with a high boiling point is alsoapplicable. ULBON SPX-1 (SUS) For the separation of impurities (for instance, m-xylene, Cumene and etc.) of p-xylene.SPX-1 is a stainless steel (SUS) capillary column coated with stationary phase of thehalogenation phthalic acid ester structure (WCOT) .As for the analysis of m-xylene in p-xylene, the capillary column analysis is said to be a verydifficult analysis so that p-xylene is eluted and then m-xyrene in a usual GC analysis.Especially, small amount analysis of m-xylene is said to be an extremely difficult. m-Xyleneof a small amount in front of p-xylene is eluted on the SPX-1. Therefore, the quantitativeanalysis is possible.4

ULBON Series SpecificationULBON HR Series (General purpose type)ColumnStationary PhasePolarityMaximumTemperatureUsageHR-1(Corresponding OV-1, OV-101)Dimethyl silicone resinNon330General analysisHR-52(Corresponding SE-52)5PhenylmethylsiliconeLow330General analysisHR-17(Corresponding OV-17)HR-1701(Corresponding OV-1701)50Phenylmethylsilicone14 Cyanopropylphenylmethyl siliconeMid300280Medicine, saccharide, TMS derivatives,phenols, steroids, and solventsHR-20M(Corresponding PEG-20M)PolyethyleneglycolHigh240General analysis, polar compounds,esters, ketones and alcoholsULBON HR Series (Special purpose type)ColumnStationary PhasePolarityMaximumTemperatureUsageHR-SS-10Nitrile siliconeHigh220/230Fatty acid ester(especially, geometric isomer of C1=18 andC2=18)HR-Thermon-3000BPhthalic acid esterHigh220/230Fatty acid esters(analysis of unsaturated C20)HR-Thermon-600TPhthalic esterHigh240Essential oil, PerfumeHR-Thermon-HGAlkyleneglycolPhthalic acid esterpolymerHigh160Methylmercury, Ethyl mercury,Toribtyl tinHR-TGC1Phenylmethyl siliconeLow360/390TriglycerideULBON WCOT Series (Original)ColumnMaximumTemperatureUsageULBON WCOT Series (SUS)ColumnMaximumTemperatureUsageALPhenDMN 267PLC160 160 160 Alkyl phenolsDimethylnaphthalenes(separation of 2, 6- DMN,2, and 7- DMN)Separation of all isomersof dimethylnaphthaleneSPX-1Squalane110 70 m-Xylene in p-XyleneThe exclusive column forxylene isomer analysisHydrocarbon (Rigroin)The exclusive column forlow hydrocarbon analysisXylene Master160 Separation of isomer ofxylenAdvance-DS220 Fatty acid methylic estersFFAP180 Lower free fatty acids5

(Yen)(Yen)99,0003099,0003061,00015561,00015599,0003099,0003061,00015361,00015399,0003099,0003061,00015261,00015299,0003099,0003061,0001510.5361,0001510.53130,00050130,0005088,0003088,0003075,00025175,000251130,00050130,0005088,0003088,0003075,000250.575,000250.5130,00050130,0005088,0003088,0003075,000250.2575,000250.25130,00050130,0005088,0003088,0003075,000250.150.3275,000250.150.32127,00050127,0005077,0003077,0003069,000250.569,000250.5127,00050127,0005077,0003077,0003069,000250.2569,000250.25127,00050127,0005077,0003077,0003069,000250.150.2533069,000250.150.25330127,00050(CorrespondingSE-52127,00050(CorrespondingOV-1, OV-101)69,000250.250.2HR-5269,000250.250.2HR-1(m)(m)(mm)(m)(m)(mm)PriceLengthFilmThicknessInnerDiameterColumnPriceLengthFilmThicknessInnerDiameterColumnULBON HR Series (General purpose type)Fixed Price ListULBON Series6

Fixed Price ListULBON Series(Yen)(Yen)99,0003061,00015599,0003099,0003061,00015561,00015399,0003099,0003061,00015361,00015299,0003099,0003061,00015261,0001510.5399,00030130,0005061,0001510.5388,00030130,0005075,00025188,00030130,0005075,00025188,00030130,0005075,000250.588,00030130,0005075,000250.588,00030130,0005069,000250.2588,00030130,0005075,000250.2588,00030130,0005075,000250.150.3288,00030127,0005075,000250.150.3277,00030127,0005069,000250.577,00030127,0005069,000250.577,00030127,0005069,000250.2577,00030127,0005069,000250.2577,00030127,0005069,000250.150.2528077,00030127,00050(CorrespondingOV-170169,000250.150.2524069,000250.250.2HR-1701127,00050(CorrespondingPEG-20M 127,00050(CorrespondingOV-17 30069,000250.250.2HR-20M69,000250.250.25HR-17(m)(m)(mm)(m)(m)(mm)PriceLengthFilmThicknessInnerDiameterColumnPriceLengthFilmThicknessInnerDiameterColumnULBON HR Series (General purpose type )7

ULBON SeriesFixed Price ListULBON HR Series (Special purpose type)ColumnInnerdiameter(mm)Length(m)Price(Yen)ColumnInnerdiameter(mm)Length(m)Price(Yen)HR-SS-100.252569,000HR-Thermon-600T0.252569,000220 /230 3083,000240 /240 3082,00050127,00050126,0000.532579,000HR-Thermon-HG0.531575,0003091,000160 50149,000*HR-TGC10.253059,000HR-Thermon-3000B0.252569,000360 /390 220 /230 3083,00050127,0000.322577,0003088,00050143,0000.531566,00030108,000ULBON WCOT Series (Original)ColumnInnerDiameter(mm)Length(m)Price(Yen)ColumnInnerDiameter(mm)Length(m)Price(Yen)ALPhen160 0.20.325050108,000132,000Xylene Master160 0.325069,000DMN 267160 0.250198,000Advance-DS0.250.25253069,00077,000PLC160 0.2550180,000220 0.2550126,000ULBON WCOT Series (SUS)ColumnInnerDiameter(mm)Length(m)Price(Yen)SPX-110110 0.256090107,800140,8008

Applications directoryApplicationsPageHydrocarbons…10Organic Solvents…18Alcohols…22Triglyceride…23Fatty Acids and Esters…24Perfume…31Flower fragrance using NeedlEx for organic solvent…32Food flavor using NeedlEx for organic solvent…34Steroids…35Environmental Pollutants37Others41Application Index…42Product Index…519

HydrocarbonsParaffin wax (m.p. 68 70 )1. n-Hexacosane (C26)2. n-Hexatriacontane (C36)21Column : ULBON HR-1 25 m x 0.25 mm I.D. df = 0.15mColumn temp : 200 320 Program rate 6 /minInjection : 330 (Sprit ratio: 53 :1)Detector : FID (330 )Carrier gas : He, 0.74 mL/minSample Volume: 1.0LParaffin wax (m.p. 48 50 )1. n-Eicosane (C20)2. n-Pentacosane (C25)21Column : ULBON HR-1 25 m x 0.25 mm I.D. df = 0.15mColumn temp : 200 320 Program rate 6 /minInjection : 330 (Sprit ratio: 53 :1)Detector : FID (330 )Carrier gas : He, 0.74 mL/minSample Volume: 0.5 L10

HydrocarbonsParaffin wax (m.p. 68 70 )1. n-Hexacosane (C26)2. n-Hexatriacontane (C36)21Column : ULBON HR-52 25 m x 0.25 mm I.D. df = 0.15mColumn temp : 200 320 Program rate 6 /minInjection : 330 (Sprit ratio: 56 :1)Detector : FID (330 )Carrier gas : He, 0.7 mL/minSample Volume: 1.0LParaffin wax (m.p. 48 50 )1. n-Eicosane (C20)2. n-Pentacosane (C25)21Column : ULBON HR-52 25 m x 0.25 mm I.D. df = 0.15mColumn temp : 200 320 Program rate 6 /minInjection : 330 (Sprit ratio: 56 :1)Detector : FID (330 )Carrier gas : He, 0.7 mL/minSample Volume: 0.5 L11

HydrocarbonsRegular gasoline1 2 345789101. n-Pentane (n-C5)2. n-Hexane (n-C6)3. Benzene4. iso-Octane (iso-C8)5. Toluene6. Ethylbenzene7. m, p-Xylene8. o-Xylene9. m-Ethyltoluene10. 1,2,4-Trimethylbenzene6Column : ULBON HR-1 50 m x 0.25 mm I.D. df = 0.5mColumn temp : 60 220 Program rate 4 /minInjection : 250 (Sprit ratio: 200 :1)Detector : FID (250 )Carrier gas : He, 0.75 mL/minSample Volume: 0.5 LAromatic hydrocarbons in Regular gasoline123 4 5710911131. Benzene2. Toluene3. Ethylbenzene4. p-Xylene5. m-Xylene6. iso-Propylbenzene7. o-Xylene8. n-Propylbenzene9. p-Ethyltoluene10. m-Ethyltoluene11. o-Ethyltoluene12. 1,2,5-Trimethylbenzene13. 1,2,4-Trimethylbenzene14. 1,2,3-Trimethylbenzene15. Naphthalene81214615Column : ULBON HR-20M 50 m x 0.25 mm I.D. df = 0.5mColumn temp : 50 180 Program rate 2 /minInjection : 250 (Sprit ratio: 200 :1)Detector : FID (250 )Carrier gas : He, 1.04 mL/minSample Volume: 0.2 L12

HydrocarbonsPremium gasoline134 59 101. n-Pentane (n-C5)2. n-Hexane (n-C6)3. Benzene4. iso-Octane (iso-C8)5. Toluene6. Ethylbenzene7. m, p-Xylene8. o-Xylene9. m-Ethyltoluene10. 1,2,4-Trimethylbenzene2786Column : ULBON HR-1 50 m x 0.25 mm I.D. df = 0.5mColumn temp : 60 220 Program rate 4 /minInjection : 250 (Sprit ratio: 200 :1)Detector : FID (250 )Carrier gas : He, 0.75 mL/minSample Volume: 0.5LAromatic hydrocarbons in Premium gasoline12543713109 11128141. Benzene2. Toluene3. Ethylbenzene4. p-Xylene5. m-Xylene6. iso-Propylbenzene7. o-Xylene8. n-Propylbenzene9. p-Ethyltoluene10. m-Ethyltoluene11. o-Ethyltoluene12. 1,2,5-Trimethylbenzene13. 1,2,4-Trimethylbenzene14. 1,2,3-Trimethylbenzene15. Naphthalene615Column : ULBON HR-20M 50 m x 0.25 mm I.D. df = 0.5mColumn temp : 50 180 Program rate 2 /minInjection : 250 (Sprit ratio: 200 :1)Detector : FID (250 )Carrier gas : He, 1.04 mL/minSample Volume: 0.2 L13

HydrocarbonsPolycyclic aromatic hydrocarbons1 2 345678910111213141. Naphthalene2. Biphenyl3. Fluorene4. Phenanthrene5. Anthracene6. o-Terphenyl7. Fluoranthene8. Pyrene9. m-Terphenyl10. p-Terphenyl11. Chrysene12. 4,5-Benzpyrene13. 1,2-Benzpyrene14. PeryleneColumn : ULBON HR-1 50 m x 0.25 mm I.D. df = 0.25mColumn temp : 200 320 Program rate 4 /minInjection : 330 (Sprit ratio: 60 :1)Detector : FID (330 )Carrier gas : He, 0.68 mL/minSample Volume: 1.0LStyrene monomer (10ppm) in DMF81. Benzene2. Acrylonitrile3. Toluene4. Ethylbenzene5. iso-Propylbenzene6. n-Propylbenzene7. Styrene8. N,N-Dimethylformamide132456 7Column : ULBON HR-20M 30 m x 0.53 mm I.D. df = 5.0mColumn temp : 120 Injection : 200 (Direct)Detector : FID (200 )Carrier gas : He, 10 mL/minSample Volume: 0.5L14

HydrocarbonsDimethylnaphtalene (DMN) mixture12345106897 14111213151. 2-Methylnaphthalene2. 1-Methylnaphthalene3. 1-Ethylnaphthalene4. 2-Ethylnaphthalene5. Diphenyl6. 1,7-DMN7. 2,7-DMN8. 1,3-DMN9. 2,6-DMN10. 1,6-DMN11. 1,4-DMN12. 1,5-DMN13. 2,3-DMN14. 1,2-DMN15. 1.8-DMNColumn : ULBON PLC 50 m x 0.25 mm I.D.Column temp : 155 Injection : 200 (Sprit ratio: 70 :1)Detector : FID (200 )Carrier gas : N2, 0.46 mL/minSample Volume: 1.0L2,6-, 2,7-Dimethylnaphtalene13451. 2,6-DMN2. 2,7-DMN3. 1,3-DMN4. 1,4-DMN5. 1,5-DMN6. 1,2-DMN7. 2.3-DMN8. 1,8-DMN2768Column : ULBON DMN267 50 m x 0.20 mm I.D.Column temp : 160 Injection : 250 (Sprit ratio: 70 :1)Detector : FID (250 )Carrier gas : N2, 0.42 mL/minSample Volume: 1.0 L15

HydrocarbonsAlkyl benzene isomers1 2 3 45768 9 10 11 12 13 14 1516 17 181. n-Hexane2. Benzene3. n-Octane4. Toluene5. n-Nonane6. Ethylbenzene7. p-Xylene8. m-Xylene9. o-Xylene10. Isopropylbenzene11. Styrene12. n-Propylbenzene13. p-Ethyltoluene14. m-Ethyltoluene15. o-Ethyltoluene16. 1,3,5-Trimethylbenzene17. 1,2,4-Trimethylbenzene18. 1,2,3-Trimethylbenzene19. 4-n-Propyltoluene20. 3-n-Propyltoluene21. 2-n-Propyltoluene22. 1,2,3,5-Tetramethylbenzene23. 1,2,4,5-Tetramethylbenzene24. 1,2,3,4-Tetramethylbenzene19 20 2321 2422Column : ULBON Xylene Master 50 m x 0.32 mm I.D.Column temp : 70 120 Program rate 2 /minInjection : 200 (Sprit ratio: 29 :1)Detector : FID (200 )Carrier gas : He, 1.4 mL/minSample Volume: 1.0 LEthylbenzene1. Ethylbenzene (99.66 %)2. p-Xylene (2790 ppm)3. o-Xylene (210 ppm)123Column : ULBON Xylene Master 50 m x 0.32 mm I.D.Column temp : 70 Injection : 200 (Sprit ratio: 55 :1)Detector : FID (200 )Carrier gas : He, 1.2 mL/minSample Volume: 1.0 L16

Hydrocarbonsp-Xylenem-Xylene31. Toluene (90 ppm)2. Ethylbenzene (1040 ppm)3. p-Xylene (99.71 %)4. m-Xylene (1210 ppm)5. o-Xylene (580 ppm)31. Toluene (550 ppm)2. p-Xylene (1850 ppm)3. m-Xylene (99.49 %)4. o-Xylene (2630 ppm)412451 2o-XyleneColumn : ULBON Xylene Master 50 m x 0.32 mm I.D.Column temp : 70 Injection : 200 (Sprit ratio: 55 :1)Detector : FID (200 )Carrier gas : He, 1.2 mL/minSample Volume: 1.0 LColumn : ULBON Xylene Master 50 m x 0.32 mm I.D.Column temp : 70 Injection : 200 (Sprit ratio: 55 :1)Detector : FID (200 )Carrier gas : He, 1.2 mL/minSample Volume: 1.0 L131. p-Xylene (6780 ppm)2. m-Xylene (740 ppm)3. o-Xylene (98.95 %)4. Isopropylbenzene(1320 ppm)5. n-Propylbenzene (760 ppm)6. p-Ethyltoluene (190 ppm)7. m-Ethyltoluene (90 ppm)4256 7m-Xylene in p-Xylene1. Toluene2. Ethylbenzene3. m-Xylene4. p-Xylene5. iso-Propylbenzene (Cumene)6. o-XyleneColumn : ULBON Xylene Master 50 m x 0.32 mm I.D.Column temp : 70 Injection : 200 (Sprit ratio: 55 :1)Detector : FID (200 )Carrier gas : He, 1.2 mL/minSample Volume: 1.0 LColumn : ULBON SPX-1 60 m x 0.25 mm I.D.Column temp : 80 Injection : 200 (Sprit ratio: 55 :1)Detector : FID (200 )Carrier gas : He, 1.1 mL/minSample Volume: 1.0 L17

Organic SolventsSolvents1 23 2067 124 10 11 211613 17 19 228 15592314182425262728 293031321. Methanol2. Acetone3. Ethyl Ether4. Dichloromethane5. trans-1,2-Dichloroethylene6. Methyl Ethyl Ketone7. 2-Butanol8. Ethyl Acetate9. Chloroform10. Tetrahydrofuran333435363711. 1,2-Dichloroethane12. 1,1,1-Trichloroethane13. n-Butanol14. Carbon Tetrachloride15. 1,4-Dioxane16. n-Propyl Acetate17. Isoamyl Alcohol18. Active Amyl Alcohol19. Isoamyl Acetate20. Toluene21. Methyl n-Butyl Ketone22. n-Butyl Acetate23. Tetrachloroethylene24. Chlorobenzene25. Ethylbenzene26. m-Xylene27. Cyclohexanone28. Styrene29. o-Xylene30. n-Amyl Acetate31. 2-Methylcyclohexanol32. 4-Methylcyclohexanone33. m-Dichlorobenzene34. p-Dichlorobenzene35. o-Dichlorobenzene36. o-Cresol37. p-CresolColumn : ULBON HR-1 50 m x 0.25 mm I.D. df = 0.5mColumn temp : 60 120 Program rate 2 /minInjection : 250 (Sprit ratio: 59 :1)Detector : FID (250 )Carrier gas : He, 0.93 mL/minSample Volume: 1.0 LSolvents2345810141. Methanol2. Ethanol3. Acetone4. 1-Propanol5. n-Hexane6. Ethyl Acetate7. Chloroform8. Benzene9. Methyl Isobutyl Kentone10. Toluene11. Isobutyl Acetate12. n-Butyl Acetate13. Ethylbenzene14. m-Xylene15. o-Xylene16. Cellosolve Acetate16911121315716Column : ULBON HR-52 25 m x 0.32 mm I.D. df = 0.5mColumn temp : 40 90 Program rate 2 /minInjection : 250 (Sprit ratio: 71 :1)Detector : FID (250 )Carrier gas : He, 0.83 mL/minSample Volume: 1.0 L18

Organic SolventsSolvents123456789111012131415161819 2117201. Methanol2. Acetone3. Dichloromethane4. trans-1,2-Dichloroethylene5. n-Hexane6. Ethyl Acetate7. Tetrahydrofuran8. 1,1,1-Trichloroethane9. Carbon Tetrachloride10. Trichloroethylene11. 1,4-Dioxane12. Methyl Isobutyl Ketone13. Toluene14. Methyl n-Butyl Ketone15. n-Butyl Acetate22232425 26272816. Tetrachloroethylene17. Chlorobenzene18. Ethylbenzene19. p-Xylene20. Styrene21. o-Xylene22. Butyl Cellosolve23. 1,1,2,2-Tetrachloroethane24. 3-Methylcyclohexanol(cis, trans)25. 2-Methylcyclohexanone26. 4-Methylcyclohexanone27. m-Dichlorobenzene28. p-Dichlorobenzene29. o-Dichlorobenzene30. o-Cresol31. p-Cresol29 30 31Column : ULBON HR-52 50 m x 0.25 mm I.D. df = 0.5mColumn temp : 60 130 Program rate 3 /minInjection : 250 (Sprit ratio: 60 :1)Detector : FID (250 )Carrier gas : He, 1.2 mL/minSample Volume: 1.0 LSolvents14235 7691011121314151617181. Methanol2. Ethanol3. Acetone4. 2-Propanol5. 1-Propanol6. Ethyl Acetate7. Methyl Ethyl Ketone8. Chloroform9. Benzene10. n-Butanol11. Toluene12. Methyl Isobutyl Ketone13. Isobutyl Acetate14. n-Butyl Acetate15. Ethylbenzene16. m-Xylene17. o-Xylene18. Cellosolve Acetate8Column : ULBON HR-1701 25 m x 0.32 mm I.D. df = 0.5mColumn temp : 40 90 Program rate 2 /minInjection : 250 (Sprit ratio: 78 :1)Detector : FID (250 )Carrier gas : He, 0.83 mL/minSample Volume: 1.0 L19

Organic SolventsSolvents2 43 7105 141 6 9 151311128161718192021222423251. Methanol2. Ethyl Ether3. 2-Propanol4. n-Hexane16. 1,4-Dioxane17. Ethyl Cellosolve18. Toluene19. Isoamyl Alcohol5. trans-1,2-Dichloroethylene 20. Tetrachloroethylene276. Ethyl Acetate7. Methyl Ethyl Ketone8. Chloroform9. Isopropyl Acetate10. Isobutanol11. 1,2-Dichloroethane12. Methyl Cellosolve13. Trichloroethylene14. n-Butanol15. n-Propyl Acetate21. n-Butyl Acetate22. Methyl n-Butyl Ketone23. Ethylbenzene24. p-Xylene25. Isoamyl Acetate26. o-Xylene27. Styrene28. n-Amyl Acetate29. Cyclohexanol30. Butyl Cellosolve36 31. Cyclohexanone3138 394128 37 32. 1,1,2,2-Tetrachloroethane29 4033. 2-Methylcyclohexanol(cis, trans)34 3534. 2-Methylcyclohexanone3335. 3-Methylcyclohexanone3036. m-Dichlorobenzene37. p-Dichlorobenzene3238. o-Dichlorobenzene39. o-Cresol40. p-Cresol41. m-Cresol26Column : ULBON HR-1701 50 m x 0.25 mm I.D. df = 0.5mColumn temp : 60 180 Program rate 4 /minInjection : 250 (Sprit ratio: 65 :1)Detector : FID (250 )Carrier gas : He, 1.4 mL/minSample Volume: 1.0 LSolvents12374 56810912111413151816 1719202221232426252728293031321. n-Hexane2. Ethyl Ether3. Acetone4. Methyl Acetate5. trans-1,2-Dichloroethylene6. Methanol7. Isopropyl Acetate8. 2-Propanol9. Dichloromethane10. n-Propyl Acetate11. cis-1,2-Dichloroethylene12. Methyl Isobutyl Ketone13. Chloroform14. Toluene15. 1,4-Dioxane33 34 353616. Isobutanol17. Isoamyl Acetate18. Ethylbenzene19. p-Xylene20. m-Xylene21. Methyl Cellosolve22. o-Xylene23. Ethyl Cellosolve24. Styrene25. Cellosolve Acetate26. N,N-Dimethylformamide27. 4-Methylcyclohexanone28. Butyl Cellosolve29. cis-4-Methylcyclohexanol30. m-Dichlorobenzene31. p-Dichlorobenzene32. trans-4-Methylcyclohexanol33. o-Dichlorobenzene34. o-Cresol35. p-Cresol36. m-CresolColumn : ULBON HR-20M 50 m x 0.25 mm I.D. df = 0.5mColumn temp : 60 240 Program rate 3 /minInjection : 250 (Sprit ratio: 60 :1)Detector : FID (250 )Carrier gas : He, 1.3 mL/minSample Volume: 1.0 L20

Organic SolventsThe work environment21 4356789101112131415 1617 18 1920 21 222324251. Methanol (70 ppm)2. Acetone (39 ppm)3. Dichloromethane (27 ppm)4. trans-1,2-Dichloroethylene(23 ppm)5. n-Hexane (26 ppm)6. Ethyl Acetate (25 ppm)7. Tetrahydrofuran (31 ppm)8. 1,1,1-Trichloroethane (17 ppm)9. Carbon Tetrachloride (15 ppm)10. Trichloroethylene (17 ppm)11. Methyl Isobutyl Ketone (22 ppm)12. Toluene (24 ppm)13. Methyl n-Butyl Ketone (22 ppm)14. Tetrachloroethylene (14 ppm)15. Chlorobenzene (30 ppm)16. Ethylbenzene (21 ppm)17. p-Xylene (21 ppm)18. Styrene (22 ppm)19. 1,1,2,2-Tetrachloroethane (13 ppm)20. 2-Methylcyclohexanone (20 ppm)21. 4-Methylcyclohexanone (20 ppm)22. m-Dichlorobenzene (15 ppm)23. p-Dichlorobenzene (5 ppm)24. o-Cresol (10 ppm)25. p-Cresol (7 ppm)Column : ULBON HR-52 30 m x 0.53 mm I.D. 5.0mColumn temp : 60 130 Program rate 3 /minInjection : 250 (Direct)Detector : FID (150 )Carrier gas : He, 8.0 mL/minSample Volume: 1.0 mLThe work environment1234119758101261314 15 16171. Methanol (70 ppm)2. Ethyl Ether (30 ppm)3. 2-Propanol (37 ppm)4. trans-1,2-Dichloroethylene(23 ppm)5. Ethyl Acetate (25 ppm)6. Chloroform (19 ppm)7. Isobutanol (30 ppm)8. Trichloroethylene (17 ppm)9. n-Butanol (30 ppm)10. 1,4-Dioxane (25 ppm)2418192021222311. Toluene (24 ppm)12. Tetrachloroethylene (14 ppm)13. n-Butyl Acetate (19 ppm)14. Ethylbenzene (21 ppm)15. p-Xylene (21 ppm)16. Isoamyl Acetate (17 ppm)17. o-Xylene (21 ppm)18. n-Amyl Acetate (17 ppm)19. Cyclohexanol (22 ppm)20. Cyclohexanone (23 ppm)21. 2-Methylcyclohexanol (10 ppm)22. 2-Methylcyclohexanone (20 ppm)23. p-Dichlorobenzene (15 ppm)24. o-Dichlorobenzene (20 ppm)25. o-Cresol (10 ppm)26. m-Cresol (10 ppm)2526Column : ULBON HR-1701 30 m x 0.53 mm I.D. df = 5.0mColumn temp : 60 180 Program rate 4 /minInjection : 250 (Direct)Detector : FID (200 )Carrier gas : He, 10.0 mL/minSample Volume: 1.0 mL21

AlcoholsHigher alcohols in Calnaubawax1. n-Octadecanol (C18)2. n-Docosanol (C22)21Column : ULBON HR-52 25 m x 0.25 mm I.D. df = 0.25mColumn temp : 200 320 Program rate 5 /minInjection : 330 (Sprit ratio: 66 :1)Detector : FID (330 )Carrier gas : He, 0.5 mL/minSample Volume: 1.2LScotch whisky35671. Acetaldehyde2. Ethyl Acetate3. Ethanol4. 1-Propanol5. 3-Pentanol6. Active Amyl Alcohol7. Isoamyl Alcohol241Column : ULBON HR-20M 50 m x 0.32 mm I.D. df = 1.0mColumn temp : 60 240 Program rate 5 /minInjection : 250 (Sprit ratio: 10 :1)Detector : FID (250 )Carrier gas : He, 4.0 mL/minSample Volume: 0.6 L22

TriglycerideButterColumn : ULBON HR-TGC-1 30 m x 0.25 mm I.D.Column temp : 250 380 Program rate 5 /minInjection : 380 (Sprit ratio: 45 :1)Detector : FID (380 )Carrier gas : He, 1.5 mL/minSample Volume: 1.0 LSalmon oilColumn : ULBON HR-TGC-1 30 m x 0.25 mm I.D.Column temp : 25 380 Program rate 5 /minInjection : 380 (Sprit ratio: 45 :1)Detector : FID (380 )Carrier gas : He, 1.5 mL/minSample Volume: 1.0 L23

Fatty Acids and EstersLower fatty acids in water1. Acetic Acid2. Propionic Acid3. iso-Butyric Acid4. n-Butyric Acid5. iso-Valeric Acid6. iso-Caproic Acid7. n-Caproic Acid8. n-Heptanoic Acid2 3 4516 78Column : ULBON HR-20M 30 m x 0.53 mm I.D. df = 3.0 mColumn temp : 150 Injection : 250 (Direct)Detector : FID (250 )Carrier gas : He, 10.5 mL/minSample Volume: 0.4 LHigher fatty acid esters in Calnaubawax351. Methyl Arachidate (C20:0)2. Methyl Behenate (C22:0)3. Methyl Lignocerate (C24:0)4. Methyl Cerotate (C26:0)5. Methyl Montanate (C28:0)6. Methyl Melissylate (C30:0)1246Column : ULBON HR-52 25 m x 0.25 mm I.D. 0.25mColumn temp : 200 320 Program rate 5 /minInjection : 330 (Sprit ratio: 61 :1)Detector : FID (330 )Carrier gas : He, 0.5 mL/minSample Volume: 1.0 L24

Fatty Acids and EstersFatty acid methyl esters1234567810111213141. Methyl Caproate (C6:0)2. Methyl Laurate (C12:0)3. Methyl Myristate (C14:0)4. Methyl Palmitate (C16:0)5. Methyl Palmitoleate (C16:1)6. Methyl Stearate (C18:0)7. Methyl Oleate (C18:1n9c)8. Methyl Linoleate (C18:2n6c)9. Methyl Linolenate (C18:3n3)10. Methyl Arachidate (C20:0)11. Methyl cis-11-Eicosenoate (C20:1n9)12. Methyl Behenate (C22:0)13. Methyl Erucate (C22:1n9)14. Methyl Lignocerate (C24:0)15. Methyl Nervonate (C24:1n9)915Column : ULBON HR-20M 25 m x 0.25 mm I.D. df = 0.25mColumn temp : 130 240 Program rate 4 /minInjection : 250 (Sprit ratio: 40 :1)Detector : FID (250 )Carrier gas : He, 1.1 mL/minSample Volume: 1.0 LFatty acid methyl esters standard12 34567891. Methyl Caprate (C10:0)2. Methyl Laurate (C12:0)3. Methyl Myristate (C14:0)4. Methyl Palmitate (C16:0)5. Methyl Palmitoleate (C16:1)6. Methyl Stearate (C18:0)7. Methyl Oleate (C18:1n9c)8. Methyl Linoleate (C18:2n6c)9. Methyl Linolenate (C18:3n3)10. Methyl Arachidate (C20:0)11. Methyl cis-11-Eicosenoate (C20:1n9)12. Methyl Behenate (C22:0)13. Methyl Erucate (C22:1n9)14. Methyl cis-4,7,10,13,16,19-Docosahexaenoate (C22:6n3)15. Methyl Lignocerate (C24:0)16. Methyl Nervonate (C24:1n9)10111213151416Column : ULBON HR-Thermon-3000B 50 m x 0.25 mm I.D.Column temp : 180 220 Program rate 1 /minInjection : 250 (Sprit ratio: 50 :1)Detector : FID (250 )Carrier gas : He, 1.7 mL/minSample Volume: 1.0 L25

Fatty Acids and EstersFatty acid methyl esters standard13 4265789 10 11121618231314 15 17 2019 21 221. Methyl Butyrate (C4:0)2. Methyl Caproate (C6:0)3. Methyl Caprylate (C8:0)4. Methyl Caprate (C10:0)5. Methyl Undecanoate (C11:0)6. Methyl Laurate (C12:0)7. Methyl Tridecanoate (C13:0)8. Methyl Myristate (C14:0)9. Methyl Myristoleate (C14:1)10. Methyl Pentadecanoate (C15:0)11. Methyl cis-10-Pentadecanoate (C15:1)12. Methyl Palmitate (C16:0)13. Methyl Palmitoleate (C16:1)14. Methyl Heptadecanoate (C17:0)15. Methyl cis-10-Heptadecenoate (C17:1)16. Methyl Stearate (C18:0)17. Methyl Elaidate (C18:1n9t)18. Methyl Oleate (C18:1n9c)353029283624 25 2631 33 3427323719. Methyl Linolelaidate (C18:2n6t)20. Methyl Linoleate (C18:2n6c)21. Methyl -Linolenate (C18:3n6)22. Methyl -Linolenate (C18:3n3)23. Methyl Arachidate (C20:0)24. Methyl cis-11-Eicosenoate (C20:1n9)25. Methyl Henicosanoate (C21:0)26. Methyl cis-11,14-Eicosadienoate(C20:2)27. Methyl cis-8,11,14-Eicosatrienoate(C20:3n6)28. Methyl Arachidonate (C20:4n6)29. Methyl cis-,11,14,17-Eicosatrienoate(C20:3n3)30. Methyl Behenate (C22:0)31. Methyl Erucate (C22:1n9)32. Methyl cis-5,8,11,14,17-Eicosapentaenoate (C20:5n3)33. Methyl Tricosanoate (C23:0)34. Methyl cis-13,16-Docosadienoate(C22:2)35. Methyl Lignocerate (C24:0)36. Methyl Nervonate (C24:1n9)37. Methyl cis-4,7,10,13,16,19-Docosahexaenoate (C22:6n3)Column : ULBON HR-SS-10 50 m x 0.25 mm I.D.Column temp : 160 220 Program rate 1.5 /minInjection : 250 (Sprit ratio: 60 :1)Detector : FID (250 )Carrier gas : He, 1.0 mL/minSample Volume: 1.0 LFatty acid methyl esters standard123456789 1011121314151617181. Methyl Caprate (C10:0)2. Methyl Laurate (C12:0)3. Methyl Myristate (C14:0)4. Methyl Palmitate (C16:0)5. Methyl Palmitoleate (C16:1)6. Methyl Stearate (C18:0)7. Methyl Elaidate (C18:1n9t)8. Methyl Oleate (C18:1n9c)9. Methyl Linolelaidate (C18:2n6t)10. Methyl Linoleate (C18:2n6c)11. Methyl -Linolenate (C18:3n3)12. Methyl Arachidate (C20:0)13. Methyl cis-11-Eicosenoate (C20:1n9)14. Methyl Behenate (C22:0)15. Methyl Erucate (C22:1n9)16. Methyl Lignocerate (C24:0)17. Methyl Nervonate (C24:1n9)18. Methyl cis-4,7,10,13,16,19-Docosahexaenoate (C22:6n3)Column : ULBON HR-SS-10 50 m x 0.32 mm I.D.Column temp : 150 220 Program rate 3 /minInjection : 250 (Sprit ratio: 25 :1)Detector : FID (250 )Carrier gas : He, 3.4 mL/minSample Volume: 1.0 L26

Fatty Acids and EstersYoung yellowtail oil1 2 34589101. Methyl Myristate (C14:0)2. Methyl Palmitate (C16:0)3. Methyl Palmitoleate (C16:1)4. Methyl Stearate (C18:0)5. Methyl Oleate (C18:1n9c)6. Methyl Linoleate (C18:2n6c)7. Methyl -Linolenate (C18:3n3)8. Methyl cis-5,8,11,14,17-Eicosapentaenoate (C20:5n3)9. Methyl cis-7,10,13,16,19-Docosapentaenoate (C22:5n3)10. Methyl cis-4,7,10,13,16,19-Docosahexaenoate (C22:6n3)67Column : ULBON HR-SS-10 25 m x 0.25 mm I.D.Column temp : 150 220 Program rate 2 /minInjection : 250 (Sprit ratio: 60 :1)Detector : FID (250 )Carrier gas : He, 1.5 mL/minSample Volume: 1.0 LTuna oil1 2 3458101. Methyl Myristate (C14:0)2. Methyl Palmitate (C16:0)3. Methyl Palmitoleate (C16:1)4. Methyl Stearate (C18:0)5. Methyl Oleate (C18:1n9c)6. Methyl Linoleate (C18:2n6c)7. Methyl -Linolenate (C18:3n3)8. Methyl cis-5,8,11,14,17-Eicosapentaenoate (C20:5n3)9. Methyl cis-7,10,13,16,19-Docosapentaenoate (C22:5n3)10. Methyl cis-4,7,10,13,16,19-Docosahexaenoate (C22:6n3)679Column : ULBON HR-SS-10 25 m x 0.25 mm I.D.Column temp : 150 220 Program rate 2 /minInjection : 250 (Sprit ratio: 60 :1)Detector : FID (250 )Carrier gas : He, 1.5 mL/minSample Volume: 1.0 L27

Fatty Acids and EstersYellowtail oilCarp oil2561. Methyl Myristate (C14:0)2. Methyl Palmitate (C16:0)3. Methyl Palmitoleate (C16:1)4. Methyl Stearate (C18:0)5. Methyl Oleate (C18:1n9c)6. Methyl Linoleate (C18:2n6c)7. Methyl -Linolenate(C18:3n3)8. Methyl cis-5,8,11,14,17-Eicosapentaenoate(C20:5n3)9. Methyl cis-7,10,13,16,19-Docosapentaenoate(C22:5n3)10. Methyl cis-4,7,10,13,16,19-Docosahexaenoate(C22:6n3)23561. Methyl Myristate (C14:0)2. Methyl Palmitate (C16:0)3. Methyl Palmitoleate (C16:1)4. Methyl Stearate (C18:0)5. Methyl Oleate (C18:1n9c)6. Methyl Linoleate (C18:2n6c)7. Methyl -Linolenate (C18:3n3)13810479147Column : ULBON HR-SS-10 50 m x 0.25 mm I.D.Column temp : 150 220 Program rate 3 /minInjection : 250 (Sprit ratio: 60 :1)Detector : FID (250 )Carrier gas : He, 0.8 mL/minSample Volume: 1.0LColumn : ULBON HR-SS-10 25 m x 0.25 mm I.D.Column temp : 170 200 Program rate 2 /minInjection : 250 (Sprit ratio: 60 :1)Detector : FID (250 )Carrier gas : He, 1.5 mL/minSample Volume: 1.0LBeef tallowLard251. Methyl Myristate (C14:0)2. Methyl Palmitate (C16:0)3. Methyl Palmitoleate (C16:1)4. Methyl Stearate (C18:0)5. Methyl Oleate (C18:1n9c)6. Methyl Linoleate (C18:2n6c)251. Methyl Myristate (C14:0)2. Methyl Palmitate (C16:0)3. Methyl Palmitoleate (C16:1)4. Methyl Stearate (C18:0)5. Methyl Oleate (C18:1n9c)6. Methyl Linoleate (C18:2n6c)44613613Column : ULBON HR-SS-10 50 m x 0.25 mm I.D.Column temp : 150 220 Program rate 3 /minInjection : 250 (Sprit ratio: 60 :1)Detector : FID (250 )Carrier gas : He, 0.8 mL/minSample Volume: 1.0LColumn : ULBON HR-SS-10 50 m x 0.25 mm I.D.Column temp : 150 220 Program rate 3 /minInjection : 250 (Sprit ratio: 60 :1)Detector : FID (250 )Carrier gas : He, 0.8 mL/minSample Volume: 1.0L28

Fatty Acids and EstersChicken oilButter234561. Methyl Myristate (C14:0)2. Methyl Palmitate (C16:0)3. Methyl Palmitoleate (C16:1)4. Methyl Stearate (C18:0)5. Methyl Oleate (C18:1n9c)6. Methyl Linoleate (C18:2n6c)7. Methyl -Linolenate(C18:3n3)1 2 34671. Methyl Caprylate (C8:0)2. Methyl Laurate (C12:0)3. Methyl Myristate (C14:0)4. Methyl Palmitate (C16:0)5. Methyl Palmitoleate (C16:1)6. Methyl Stearate (C18:0)7. Methyl Oleate (C18:1n9c)8. Methyl Linoleate (C18:2n6c)1758Column : ULBON HR-SS-10 50 m x 0.25 mm I.D.Column temp : 150 220 Program rate 3 /minInjection : 250 (Sprit ratio: 60 :1)Detector : FID (250 )Carrier gas : He, 0.8 mL/minSample Volume: 1.0LMargarineSoybeanColumn : ULBON HR-SS-10 50 m x 0.25 mm I.D.Column temp : 150 220 Program rate 3 /minInjection : 250 (Sprit ratio: 60 :1)Detector : FID (250 )Carrier gas : He, 0.8 mL/minSample Volume: 1.0L31. Methyl Laurate (C12:0)2. Methyl Myristate (C14:0)3. Methyl Palmitate (C16:0)4. Methyl Stearate (C18:0)5. Methyl Elaidate (C18:1n9t)6. Methyl Oleate (C18:1n9c)7. Methyl Linoleate (C18:2n6c)8. Methyl -Linolenate(C18:3n3)1431. Methyl Palmitate (C16:0)2. Methyl Stearate (C18:0)3. Methyl Oleate (C18:1n9c)4. Methyl Linoleate (C18:2n6c)5. Methyl -Linolenate(C18:3n3)6 78451252Column : ULBON HR-SS-10 50 m x 0.25 mm I.D.Column temp : 150 220 Program rate 3 /minInjection : 250 (Sprit ratio: 60 :1)Detector : FID (250 )Carrier gas : He, 0.8 mL/minSample Volume: 1.0LColumn : ULBON HR-SS-10 50 m x 0.25 mm I.D.Column temp : 150 220 Program rate 3 /minInjection : 250 (Sprit ratio: 60 :1)Detector : FID (250 )Carrier gas : He, 0.8 mL/minSample Volume: 1.0L29

Fatty Acids and EstersBreast milk1234781. Methyl Caprylate (C8:0)2. Methyl Laurate (C12:0)3. Methyl Myristate (C14:0)4. Methyl Palmitate (C16:0)5. Methyl Palmitoleate (C16:1)6. Methyl Stearate (C18:0)7. Methyl Oleate (C18:1n9c)8. Methyl Linoleate (C18:2n6c)9. Methyl -Linolenate (C18:3n3)10. Methyl cis-5,8,11,14,17-Eicosapentaenoate (C20:5n3)11. Methyl cis-7,10,13,16,19-Docosapentaenoate (C22:5n3)12. Methyl cis-4,7,10,13,16,19-Docosahexaenoate (C22:6n3)569 10 1112Column : ULBON HR-SS-10 50 m x 0.25 mm I.D.Column temp : 150 220 Program rate 3 /minInjection : 250 (Sprit ratio: 60 :1)Detector : FID (250 )Carrier gas : He, 0.8 mL/minSample Volume: 1.0L30

PerfumePerfume 1 35 6 71. -Pinene2. -Pinene3. Limonene4. Linalool5. Linalyl Acetate6. Benzyl Acetate7. Phenethyl Alcohol241Column : ULBON HR-Thermon-600T 50 m x 0.25 mm I.D.Column temp : 70 230 Program rate 4 /minInjection : 250 (Sprit ratio: 80 :1)Detector : FID (250 )Carrier gas : He, 1.1 mL/minSample Volume: 1.0LPerfume 2 24356 7 8910121. -Pinene2. Limonene3. Linalool4. Linalyl Acetate5. Benzyl Acetate6. -Ionene7. Phenethyl Alcohol8. Lauryl Alcohol9. Eugenol10. Diethyl Phthalate11. Vanillin12. Benzyl Salicylate111Column : ULBON HR-Thermon-600T 50 m x 0.25 mm I.D.Column temp : 70 230 Program rate 4 /minInjection : 250 (Sprit ratio: 80 :1)Detector : FID (250 )Carrier gas : He, 1.1 mL/minSample Volume: 1.0L31

Flower fragrance using NeedlEx for organic solventNarcissusFragrant daphne1. 1,8-Cineole2. -Phenethyl Alcohol3. Benzyl Acetate1. Linalool2. Anisaldehyde3. Geraniol4. Perillaldehyde311223 4Column : ULBON HR-1 30 m x 0.32 mm I.D.df = 0.25mColumn temp : 50 200 Program rate 5 /minInjection : 250 (Sprit ratio: 10 :1)Detector : FID (250 )Carrier gas : He, 2.4 mL/minColumn : ULBON HR-1 30 m x 0.32 mm I.D.df = 0.25mColumn temp : 50 200 Program rate 5 /minInjection : 250 (Sprit ratio: 10 :1)Detector : FID (250 )Carrier gas : He, 2.4 mL/minRosemaryPeppermint1. -Pinene2. Limonene3. Isobornyl Acetate4. -Ionone1. Myrcene2. 1,8-Cineole3. L-Menthol4. Anisaldehyde2 3241413Before the extractionColumn : ULBON HR-1 30 m x 0.32 mm I.D.df = 0.25mColumn temp : 50 200 Program rate 5 /minInjection : 250 (Sprit ratio: 10 :1)Detector : FID (250 )Carrier gas : He, 2.4 mL/minColumn : ULBON HR-1 30 m x 0.32 mm I.D.df = 0.25mColumn temp : 50 200 Program rate 5 /minInjection : 250 (Sprit ratio: 10 :1)Detector : FID (250 )Carrier gas : He, 2.4 mL/min32

Flower fragrance using NeedlEx for organic solventAngels trumpetLemon balm1. -Pinene2. 1,8-Cineole3. -Phenethyl Alcohol1. L-Menthon2. Anisaldehyde3. Geraniol4. L-Perillaldehyde5. -Ionone241 21335Column : ULBON HR-1 30 m x 0.32 mm I.D.df = 0.25mColumn temp : 50 200 Program rate 5 /minInjection : 250 (Sprit ratio: 10 :1)Detector : FID (250 )Carrier gas : He, 2.4 mL/minColumn : ULBON HR-1 30 m x 0.32 mm I.D.df = 0.25mColumn temp : 50 200 Program rate 5 /minInjection : 250 (Sprit ratio: 10 :1)Detector : FID (250 )Carrier gas : He, 2.4 mL/minYuzu citronHolly tree1. Myrcene2. Limonene1. Benzyl Alcohol2. -Phethyl Alcohol3. Eugenol22113Column : ULBON HR-1 30 m x 0.32 mm I.D.df = 0.25mColumn temp : 50 200 Program rate 5 /minInjection : 250 (Sprit ratio: 10 :1)Detector : FID (250 )Carrier gas : He, 2.4 mL/minColumn : ULBON HR-1 30 m x 0.32 mm I.D.df = 0.25mColumn temp : 50 200 Program rate 5 /minInjection : 250 (Sprit ratio: 10 :1)Detector : FID (250 )Carrier gas : He, 2.4 mL/min33

Food flavor using NeedlEx for organic solventSoy sauceWorcestershire sauce1231. Ethanol2. Acetic Acid3. Isoamyl ALcohol4. 3-(Methylthio)-1-propanol121. Ethanol2. Acetic Acid3. Isoamyl ALcohol43Column : ULBON HR-1 30 m x 0.32 mm I.D.df = 0.25mColumn temp : 50 200 Program rate 5 /minInjection : 250 (Sprit ratio: 10 :1)Detector : FID (250 )Carrier gas : He, 2.4 mL/minColumn : ULBON HR-1 30 m x 0.32 mm I.D.df = 0.25mColumn temp : 50 200 Program rate 5 /minInjection : 250 (Sprit ratio: 10 :1)Detector : FID (250 )Carrier gas : He, 2.4 mL/minBrandy14521. Ethanol2. 1-Propanol3. Ethyl Acetate4. Isobutanol5. Isoamyl Alcohol6. Ethyl n-Caproate36Column : ULBON HR-1 30 m x 0.32 mm I.D.df = 0.25mColumn temp : 50 200 Program rate 5 /minInjection : 250 (Sprit ratio: 10 :1)Detector : FID (250 )Carrier gas : He, 2.4 mL/min34

SteroidsPlant sterolsPlant sterols in rice bran oil41. Brassicasterol2. Campesterol3. Stigmasterol4. -Sitosterol1. Campesterol2. Stigmasterol3. -Sitosterol2 32113Column : ULBON HR-1 30 m x 0.53 mm I.D. df = 1.0mColumn temp : 290 Injection : 320 (Direct)Detector : FID (320 )Carrier gas : He, 7.1 mL/minSample Volume: 0.5LColumn : ULBON HR-52 25 m x 0.25 mm I.D. df = 0.25mColumn temp : 250 320 Program rate 5 /minInjection : 330 (Sprit ratio: 61 :1)Detector : FID (330 )Carrier gas : He, 0.5 mL/minSample Volume: 0.5LCholesterol and Plant sterols51. Cholesterol2. Brassicasterol3. Campesterol4. Stigmasterol5. -Sitosterol1342Column : ULBON HR-17 25 m x 0.25 mm I.D.df = 0.25mColumn temp : 290 Injection : 310 (Sprit ratio: 20 :1)Detector : FID (310 )Carrier gas : He, 1.5 mL/minSample Volume: 1.5 L35

SteroidsCholesterol estersCholesterol & Cholestanol12345 6781. Cholesterol2. Cholesterol Acetate3. Cholesterol Propionate4. Cholesterol Isobutyrate5. Cholesterol n-Butyrate6. Cholesterol n-Valerate7. Cholesterol n-Caproate8. Cholesterol n-Octanoate9. Cholesterol n-Decanoate10. Cholesterol Laurate11. Cholesterol Myristate11.Cholesterol2.Cholestanol910112Column : ULBON HR-1 25 m x 0.25 mm I.D. df = 0.15 mColumn temp : 250 330 Program rate 5 /minInjection : 340 (Sprit ratio: 48 :1)Detector : FID (340 )Carrier gas : He, 1.3 mL/minSample Volume: 0.5 LColumn : ULBON HR-1 50 m x 0.25 mm I.D. df = 0.25 mColumn temp : 300 Injection : 330 (Sprit ratio: 60 :1)Detector : FID (330 )Carrier gas : He, 0.65 mL/minSample Volume: 0.8 LSterols1341. Dehydroepiandrosterone2. Estrone3. Cholestane4. Progesterone5. Estriol6. Cholesterol7. Lanosterol2657Column : ULBON HR-52 50 m x 0.25 mm I.D. df = 0.25mColumn temp : 250 320 Program rate 4 /minInjection : Injection : 330 (Sprit ratio: 69 :1)Detector : FID (330 )Carrier gas : He, 0.5 mL/minSample Volume: 2.0L36

Environmental PollutantsPhthalic acid esters12 3456781. Dimethyl Phthalate2. Diethyl Phthalate3. Diallyl Phthalate4. Di-iso-Butyl Phthalate5. Di-n-Buthyl Phthalate6. Di-n-Amyl Phthalate7. Di-2-Ethylhexyl Phthalate8. Di-n-Octyl PhthalateColumn : ULBON HR-1 25 m x 0.25 mm I.D. df = 0.25mColumn temp : 180 300 Program rate 6 /minInjection : 320 (Sprit ratio: 36 :1)Detector : FID (320 )Carrier gas : He, 0.98 mL/minSample Volume: 0.5 LOrganic acid butyl esters241579111. n-Butyl Formate2. n-Butyl Acetate3. n-Butyl Propionate4. n-Butyl iso-Butyrate5. n-Butyl n-Butyrate6. n-Butyl Crotonate7. n-Butyl n-Caproate8. n-Butyl Lactate9. n-Butyl n-Caprate10. Di-n-Butyl Oxalate11. n-Butyl Benzoate12. Di-n-Butyl Malonate13. Di-n-Butyl Fumarate14. Di-n-Butyl Succinate15. Di-n-Butyl Maleate16. Di-n-Butyl Adipate17. Di-n-Butyl Malate18. Di-n-Butyl Phthalate19. Di-n-Butyl Sebacate20. Di-n-Butyl Isophthalate21. Di-n-Butyl Terephthalate22. Tri-n-Butyl Citrate368101213 1514161718 19 202122Column : ULBON HR-20M 25 m x 0.25 mm I.D. df = 0.25mColumn temp : 80 240 Program rate 4 /minInjection : Injection : 250 (Sprit ratio: 40 :1)Detector : FID (250 )Carrier gas : He, 1.1 mL/minSample Volume: 1.0 L37

Environmental PollutantsAlkylphenol isomers131013161. Phenol2. 2,6-Xylenol3. o-Cresol4. p-Cresol5. m-Cresol6. o-Ethylphenol7. 2,4,6-Trimethylphenol8. 2,4-Xylenol9. 2,5-Xylenol10. 2,3,6-Trimethylphenol11. 2,3-Xylenol12. p-Ethylphenol13. m-Ethylphenol14. 3,5-Xylenol15. 3,4-Xylenol16. 2,3,5-Trimethylphenol24 6 758912111415Column : ULBON ALPhen 50 m x 0.20 mm I.D.Column temp : 130 Injection : 250 (Sprit ratio: 70 :1)Detector : FID (250 )Carrier gas : N2, 0.39 mL/minSample Volume: 0.5 Lm-Cresol in p-Cresol ( benzene solution )31. Phenol2. o-Cresol3. p-Cresol4. m-Cresol124Column : ULBON ALPhen 50 m x 0.20 mm I.D.Column temp : 130 Injection : 250 (Sprit ratio: 50 :1)Detector : FID (250 )Carrier gas : N2, 0.39 mL/minSample Volume: 0.5 L38

Impurities in 3,5-xylenol (10% benzene solution)Environmental Pollutants111. 2,6-Xylenol2. p-Cresol3. m-Cresol4. 2,4,6-Trimethylphenol5. 2,4-Xylenol6. 2,5-Xylenol7. 2,3,6-Trimethylphenol8. 2,3-Xylenol9. p-Ethylphenol10. m-Ethylphenol11. 3,5-Xylenol12. 3,4-Xylenol10912 34 5 6 7812Column : ULBON ALPhen 50 m x 0.20 mm I.D.Column temp : 130 Injection : 250 (Sprit ratio: 50 :1)Detector : FID (250 )Carrier gas : N2, 0.39 mL/minSample Volume: 0.5 LAlkylphenol mixture (0.1 0.3% Acetone solution)12341195 6 7 8 1013 1512 1416171. Acetone2. Phenol3. 2,6-Xylenol4. o-Cresol5. p-Cresol6. m-Cresol7. o-Ethylphenol8. 2,4,6-Trimethylphenol9. 2,4-Xylenol10. 2.,5-Xylenol11. 2,3,6-Trimethylphenol12. 2,3-Xylenol13. p-Ethylphenol14. m-Ethylphenol15. 3,5-Xylenol16. 3,4-Xylenol17. 2,3,5-TrimethylphenolColumn : ULBON ALPhen 50 m x 0. 32 mm I.D.Column temp : 130 Injection : 250 (Sprit ratio: 20 :1)Detector : FID (250 )Carrier gas : He, 1.3 mL/minSample Volume: 0.5 L39

Environmental PollutantsAlkyl MercuryTri-n-Butyltin Chloride1. Methyl Mercuric Chloride(100 ppb) (40 pg)2. Ethyl Mercuric Chloride(100 ppb) (40 pg)1.Tri-n-Butyltin Chloride(100 ppb) (40 pg)211Column : ULBON HR-Thermon-HG 15 m x 0.53 mm I.D.Column temp : 150 Injection : 250 (Direct)Detector : ECD (250 )Carrier gas : N2, 4.5 mL/minSample Volume: 1.0LColumn : ULBON HR-Thermon-HG 15 m x 0.53 mm I.D.Column temp : 150 Injection : 250 (Direct)Detector : ECD (250 )Carrier gas : N2, 4.5 mL/minSample Volume: 1.0LOrganic phosphorus pesticides1. DDVP2. Methyldimethone3. Methylparathion4. Ethylparathion1235. EPN45Column : ULBON HR-1 15 m x 0.53 mm I.D. df = 1.0mColumn temp : 150 220 Program rate 6 /minInjection : 250 (Direct)Detector : FID (250 )Carrier gas : He, 10.7 mL/minSample Volume: 0.5L40

OthersAliphatic amines1231. n-Octylamine2. n-Decylamine3. Laurylamine4. n-Tetradecylamine5. Cetylamine6. Stearylamine456Column : ULBON HR-52 25 m x 0.25 mm I.D. df = 0.25mColumn temp : 150 230 Program rate 4 /minInjection : 250 (Sprit ratio: 56 :1)Detector : FID (250 )Carrier gas : He, 0.48 mL/minSample Volume: 1.5LSugars (Acetate)Medicines11. Sucrose2. Maltose3. Lactose4. Raffinose121. o-Ethoxybenzamide2. Phenacetine3. Caffeine4. Aminopyrine5. Chlorpheniramine Maleate2 343 45Column : ULBON HR-1701 25 m x 0.25 mm I.D. df=0.25mColumn temp : 180 280 Program rate 4 /minInjection : Injection : 300 (Sprit ratio: 58 :1)Detector : FID (300 )Carrier gas : He, 0.49 mL/minSample Volume: 1.0LColumn : ULBON HR-1 15 m x 0.53 mm I.D. df = 1.0mColumn temp : 180 Injection : 250 (Direct)Detector : FID (250 )Carrier gas : He, 7.2 mL/minSample Volume: 0.5L41

Application IndexAcetaldehydeAcetic AcidAcetoneAcrylonitrileActive Amyl AlcoholAminopyrinen-Amyl AcetateAnisaldehydeAnthraceneBenzene1,2-Benzpyrene4,5-BenzpyreneBenzyl AcetateBenzyl AlcoholBenzyl SalicylateBiphenylBrassicasteroln-Butanol2-Butanoln-Butyl Acetaten-Butyl Benzoaten-Butyl n-Butyraten-Butyl iso-Butyraten-Butyl n-Capraten-Butyl n-CaproateButyl Cellosolven-Butyl Crotonaten-Butyl Formaten-Butyl Lactaten-Butyl Propionaten-Butyric Acidiso-Butyric AcidCaffeineCampesterolABC2224, 3418, 19, 20, 21, 391418, 224118, 20, 2132, 331412, 13, 14, 16, 18, 19141431, 323331143518, 19, 20, 211818, 19, 20, 21, 37373737373719, 20373737372424413542

Application Indexiso-Caproic Acidn-Caproic AcidCarbon TetrachlorideCellosolve AcetateCetylamineChlorobenzeneChloroformChlorpheniramine MaleateCholestaneCholestanolCholesterolCholesterol AcetateCholesterol n-ButyrateCholesterol n-CaproateCholesterol n-DecanoateCholesterol IsobutyrateCholesterol LaurateCholesterol MyristateCholesterol n-OctanoateCholesterol PropionateCholesterol n-ValerateChrysene1,8-Cineolem-Cresolo-Cresolp-CresolCyclohexanolCyclohexanoneDDVPn-DecylamineDehydroepiandrosteroneDiallyl PhthalateDi-n-Amyl PhthalateDi-n-Buthyl PhthalateDi-n-Butyl AdipateCD242418, 19, 2118, 19, 204118, 19, 2118, 19, 20, 2141363635, 36363636363636363636361432, 3320, 21, 38, 3918, 19, 20, 21, 38, 3918, 19, 20, 21, 38, 3920, 2118, 20, 214041363737373743

Application IndexDDi-n-Butyl FumarateDi-n-Butyl IsophthalateDi-n-Butyl MalateDi-n-Butyl MaleateDi-n-Butyl MalonateDi-n-Butyl OxalateDi-n-Butyl PhthalateDi-iso-Butyl PhthalateDi-n-Butyl SebacateDi-n-Butyl SuccinateDi-n-Butyl Terephthalatem-Dichlorobenzeneo-Dichlorobenzenep-Dichlorobenzene1,2-Dichloroethanetrans-1,2-Dichloroethylenecis-1,2-Dichloroethylenetrans-1,2-DichloroethyleneDichloromethaneDiethyl PhthalateDi-2-Ethylhexyl PhthalateDimethyl PhthalateN,N-Dimethylformamide1,2-DMN1,3-DMN1,4-DMN1,5-DMN1,6-DMN1,7-DMN1.8-DMN2,3-DMN2,6-DMN2,7-DMNDi-n-Octyl Phthalate1,4-DioxaneDiphenyl373737373737373737373718, 19, 20, 2118, 19, 20, 2118, 19, 20, 2118, 20212018, 19, 2018, 19, 20, 2131, 37373714, 20151515151515151515153718, 19, 20, 211544

Application Indexn-Docosanoln-Eicosane (C20)EPNEstriolEstroneEthanolo-EthoxybenzamideEthyl AcetateEthyl n-CaproateEthyl CellosolveEthyl EtherEthyl Mercuric ChlorideEthylbenzene1-Ethylnaphthalene2-EthylnaphthaleneEthylparathionm-Ethylphenolo-Ethylphenolp-Ethylphenolm-Ethyltoluenep-Ethyltolueneo-EthyltolueneEugenolFluorantheneFluoreneGeranioln-Heptanoic Acidn-Hexacosanen-Hexanen-Hexatriacontane-IoneneDEFGHI2210, 1140363618, 19, 22, 344118, 19, 20, 21, 22, 34342018, 20, 214012, 13, 14, 16, 17, 18, 19, 20, 2115154038, 3938, 3938, 3912, 13, 16, 1712, 13, 16, 1712, 13, 1631, 33141432, 332410, 1112, 13, 16, 18, 19, 20, 2110, 1131, 32, 3345

Application IndexIIsoamyl AcetateIsoamyl AlcoholIsobornyl AcetateIsobutanolIsobutyl AcetateIsopropanolIsopropyl AcetateIsopropylbenzeneLLactoseLanosterolLauryl AlcoholLaurylamineLimoneneLinaloolLinalyl AcetateMMaltoseL-MenthonMethanolMethyl AcetateMethyl Arachidate (C20:0)Methyl Arachidonate (C20:4n6)Methyl Behenate (C22:0)2-Methyl-1-Butanol3-Methyl-1-ButanolMethyl n-Butyl KetoneMethyl Butyrate (C4:0)Methyl Caprate (C10:0)Methyl Caproate (C6:0)Methyl Caprylate (C8:0)Methyl CellosolveMethyl Cerotate (C26:0)Methyl cis-13,16-Docosadienoate (C22:2)Methyl cis-4,7,10,13,16,19-Docosahexaenoate (C22:6n3)Methyl cis-7,10,13,16,19-Docosapentaenoate (C22:5n3)18, 20, 2118, 20, 22, 343220, 21, 3418, 1920, 212016, 174136314131, 32, 3331, 32314132, 3318, 19, 20, 212024, 25, 262624, 25, 26222218, 19, 20, 212625, 2625, 2626, 29, 3020242625, 26, 27, 28, 3027, 28, 3046

Application IndexMMethyl cis-11,14-Eicosadienoate (C20:2)Methyl cis-5,8,11,14,17-Eicosapentaenoate (C20:5n3)Methyl cis-,11,14,17-Eicosatrienoate (C20:3n3)Methyl cis-8,11,14-Eicosatrienoate (C20:3n6)Methyl cis-11-Eicosenoate (C20:1n9)Methyl Elaidate (C18:1n9t)Methyl Erucate (C22:1n9)Methyl Ethyl Ketone (MEK)Methyl Henicosanoate (C21:0)Methyl Heptadecanoate (C17:0)Methyl cis-10-Heptadecenoate (C17:1)Methyl Isobutyl Ketone (MIBK)Methyl Laurate (C12:0)Methyl Lignocerate (C24:0)Methyl Linoleate (C18:2n6c)Methyl Linolelaidate (C18:2n6t)Methyl Linolenate (C18:3n3)Methyl -Linolenate (C18:3n6)Methyl Melissylate (C30:0)Methyl Mercuric ChlorideMethyl Montanate (C28:0)Methyl Myristate (C14:0)Methyl Myristoleate (C14:1)Methyl Nervonate (C24:1n9)Methyl Oleate (C18:1n9c)Methyl Palmitate (C16:0)Methyl Palmitoleate (C16:1)Methyl Pentadecanoate (C15:0)Methyl cis-10-Pentadecanoate (C15:1)Methyl Stearate (C18:0)Methyl Tricosanoate (C23:0)Methyl Tridecanoate (C13:0)Methyl Undecanoate (C11:0)2-Methylcyclohexanol3-Methylcyclohexanol(cis, trans)cis-4-Methylcyclohexanol2626, 27, 28, 30262625, 2626, 2925, 2618, 19, 2026262618, 19, 20, 2125, 26, 29, 3024, 25, 2625, 26, 27, 28, 29, 302625, 26, 27, 28, 29, 302624402425, 26, 27, 28, 29, 302625, 2625, 26, 27, 28, 29, 3025, 26, 27, 28, 29, 3025, 26, 27, 28, 29, 30262625, 26, 27, 28, 29, 3026262618, 20, 21192047

Application Indextrans-4-Methylcyclohexanol2-Methylcyclohexanone3-Methylcyclohexanone4-MethylcyclohexanoneMethyldimethone1-Methylnaphthalene2-MethylnaphthaleneMethylparathion3-(Methylthio)-1-propanolMyrceneNaphthalenen-Nonanen-Octadecanoln-Octaneiso-Octanen-Octylaminen-Pentacosanen-Pentane3-PentanolL-PerillaldehydePerillaldehydePerylenePhenacetinePhenanthrenePhenethyl Alcohol-Phenethyl AlcoholPhenol-Phethyl Alcohol-Pinene-PineneProgesterone1-Propanol2-PropanolMNOP2019, 20, 212018, 19, 20, 21401515403432, 3312, 13, 1416221612, 134110, 1112, 132233321441143132, 3338, 393331, 32, 33313618, 19, 22, 3419, 20, 2148

Application IndexPropionic Acidn-Propyl Acetaten-Propylbenzeneiso-Propylbenzene2-n-Propyltoluene3-n-Propyltoluene4-n-PropyltoluenePyreneRaffinose-SitosterolStearylamineStigmasterolStyreneSucrosem-Terphenylo-Terphenylp-Terphenyl1,1,2,2-TetrachloroethaneTetrachloroethylenen-TetradecylamineTetrahydrofuran1,2,3,4-Tetramethylbenzene1,2,3,5-TetramethylbenzeneTolueneTri-n-Butyl CitrateTri-n-Butyltin Chloride1,1,1-TrichloroethaneTrichloroethyleneTriglyceride1,2,3-Trimethylbenzene1,2,4-Trimethylbenzene1,2,5-Trimethylbenzene1,3,5-TrimethylbenzenePRST2418, 2012, 13, 14, 16, 1712, 13, 14, 17161616144135413514, 16, 18, 19, 20, 214114141419, 20, 2118, 19, 20, 214118, 19, 21161612, 13, 14, 16, 17, 18, 19, 20, 21374018, 19, 2119, 20, 212312, 13, 1612, 13, 1612, 131649

Application Index2,3,5-Trimethylphenol2,3,6-Trimethylphenol2,4,6-Trimethylphenoliso-Valeric AcidVanillinm-Xyleneo-Xylenep-Xylene2,3-Xylenol2,4-Xylenol2,5-Xylenol2,6-Xylenol3,4-Xylenol3,5-XylenolTVX38, 3938, 3938, 39243112, 13, 16, 17, 18, 19, 2012, 13, 16, 17, 18, 19, 20, 2112, 13, 16, 17, 19, 20, 2138, 3938, 3938, 3938, 3938, 3938, 3950