Pathology Collection Guide 2013 - Douglass Hanly Moir Pathology

Pathology Collection Guide 2013 - Douglass Hanly Moir Pathology Pathology Collection Guide 2013 - Douglass Hanly Moir Pathology

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10.07.2015 Views

Pathology Collection Guide

Pathology Collection Guide

Pathology Collection Guidefor DoctorsAUGUST 2013Although all details are correct at time of printing, changes to testing and collection may occur.Please see our webite www.dhm.com.au for up-to-date information.

IntroductionDear Colleague,e are pleased to provide you with a copy of ourW Pathology Collection Guide for Doctors. It is designedto be an easy-to-use reference guide to the collectionrequirements for our tests and we trust that you will find itbeneficial in your day-to-day practice.We value and welcome your feedback in relation to thispublication. If you have any comments or suggestions, pleasecontact one of our pathologists or the staff in our MarketingDepartment.Douglass Hanly Moir Pathology and Barratt & Smith Pathologyhave a long tradition of offering comprehensive, high qualitypathology services for doctors, private hospitals and nursinghomes.We are medically led practices, firmly committed to maintainingprofessional and technical excellence, personalised servicesand the highest ethical standards. Our pathologists, scientists,managers and other staff are available to assist you and weencourage you to contact us at any time.With my warm regards,Dr Colin GoldschmidtM.B., B.Ch., F.R.C.P.A., F.A.I.C.D.Chief Executive OfficerDouglass Hanly Moir Pathology & Barratt & Smith Pathology2

Specialist PathologistsChief Executive Officer 9855 5333Dr Colin GoldschmidtMedical Director 9855 5150Dr Annabelle FarnsworthPathologistsBiochemistry/Endocrinology 9855 5312Dr Grahame Caldwell (Director of Chemical Pathology / Esoteric Testing)Dr Nick Taylor (Director of Chemical Pathology / Automated Laboratory)Dr Tina YenHaematology 9855 5312Dr Elizabeth BernalDr Jonathan BlackwellDr Frances Hanly (Director of Haematology)Dr Lye Lin HoDr Peter KyleDr Ray McKinleyDr Steve MoranDr Vera StoermerDr Claudine HoHistopathology/Cytopathology 9855 5150Dr Erica AhnDr Alexandra AllendeDr Tina BaillieDr Clare BiroAdj. Prof. Fiona BonarDr Ivan BurchettDr Juliet BurnDr Simon ClarkDr Robert Cortis-JonesAdj. Prof. Warick Delprado(Director of Histopathology)Dr Francesca D'SouzaDr Joanna DingDr Melanie EdwardsDr Stephen FairyAdj. Prof. Annabelle Farnsworth(Director of Cytopathology)Dr William FelbelDr Vicki HowardDr Geoffrey HallDr Suzanne HyneAdj. Prof. Richard JaworskiDr Debra JensenDr Ken KnealeDr Robyn LevingstonDr Cathy LimDr Lisa LinDr Fiona MacleanDr Abha MalikDr Denis MoirDr Anita MuljonoDr Kambin NejadDr Helen OgleDr Justine PickettDr Jessamine ReddyDr Paul RichmondDr Jennifer RobertsProfessor Peter RussellDr Elizabeth SinclairDr Andrew TanA/Prof. Jennifer TurnerDr Cate TrebeckDr Mark J WilsherDr Helene Yimaz4

Immunology 9855 5312Dr Karl Baumgart (Director of Immunology / Molecular Biology)Dr Andrew BroadfootMolecular Biology 9855 5312Dr Karl Baumgart (Director of Immunology / Molecular Biology)Microbiology/Serology 9855 5312Dr Ian Chambers (Director of Microbiology / Serology)Dr Miriam PaulDr Michael WehrhahnRegional PathologistsDubbo 6826 5455Dr Michael HarrisonGosford 4337 3502Dr Joanna DingDr Richard HaskellDr Desmond ReddyMildura (03) 5021 1626Dr Marcella RomanOrange/Bathurst 6362 3666Dr Greg Rhodes (Laboratory Director)Dr Garry SimmonsPenrith 4734 6500Dr Theresa HarveyDr Irene NguDr Marcella Roman (Laboratory Director)Port Macquarie, Newcastle & Taree 6583 4755Dr Simon Palfreeman (Laboratory Director)Wagga Wagga 6925 0055Dr David Blaxland5

Laboratory locationsMain LaboratoriesDouglass Hanly Moir Pathology14 Giffnock AvenueMacquarie Park NSW 21139855 5222 or 1800 222 365Barratt & Smith Pathology31 Lawson StreetPenrith NSW 27504734 6500 or 1800 048 993Metropolitan LaboratoriesCrows NestThe Mater HospitalLower Ground Floor, Rocklands RoadCrows Nest NSW 20659922 7805LiverpoolGround Floor,16-18 Bigge StreetLiverpool NSW 21708778 1999Dee WhyDelmar Private Hospital14 Patey StDee Why NSW 20999981 3215Macquarie University HospitalMacquarie University Clinic BuildingSuite 205, Level 22, Technology PlaceMacquarie University NSW 21099812 3655HurstvilleHurstville Community Private HospitalLevel 1, 2 Pearl StHurstvillle NSW 22209585 9503WestmeadWestmead Private HospitalCnr Mons & Darcy RoadsWestmead NSW 21459687 1888KogarahSt George Private Hospital & Medical CentreLevel 2, 1 South StreetKogarah NSW 22179553 9888WindsorHawkesbury District HospitalCnr Day & Macquarie StreetsWindsor NSW 27564560 55326

Regional LaboratoriesBathurstBathurst Private HospitalGormans Hill RoadBathurst NSW 27956332 8945Port Macquarie87 Lord StreetPort Macquarie NSW 24446589 2900Dubbo223A Darling StreetDubbo NSW 28306826 5455Tamworth199 Peel StreetTamworth NSW 23406766 2401Gosford37 William StreetGosford NSW 22504337 3500Taree1/65 Pulteney StreetTaree NSW 24306551 5453Newcastle – GatesheadMacquarie Private HospitalMedical Centre, Cnr O’Brien & Sydney StGateshead NSW 22904904 9600TorontoToronto Private HospitalSuite 3, Medical Centre 2, Excelsior ParadeToronto NSW 22834959 6599Mildura127 Langtree AvenueMildura VIC 350003 5021 1626Wagga WaggaCalvary HospitalSt Gerard's Wing, Hardy AvenueWagga Wagga NSW 26506925 0055Orange7-9 Dora StreetOrange NSW 28006362 36667

Medicare guidelines for repeat testingDrugs entitlement for patient having 6 visits within 6 monthsTest requestedFBC (& if requested ESR)Accepted drug treatmentActemra (Tocilizumab)Arava / Arabloc (Leflunomide)Aromasin (Exemestane)AtgamAvastin (Bevacizumab)Azamun (Azathioprine)AzathioprineBetaferon / Roferon-A / Rebif /(Interferon)Celebrex (Celecoxib)CellCept / Myfortic(Mycophenolate)ChemotherapyCicloral / Neoral (Cyclosporin)Cimzia (Certolizumab)Clozaril / Clopine (Clozapine)Cycloblastin (Cyclophosphamide)CyclosporinCytotoxic therapyD-penamine (Penicillamine)Enbrel (Etanercept)Faslodex (Fulvestrant)Fludara (Fludarabine)Gilenya (Fingolimod)Glivec (Imatinib)GoldHerceptin (Trastuzumab)Humira (Adalimumab)Hydrea (Hydroxyurea)Imuran (Azathioprine)Infliximab (Remicade)InterferonLeukeran (Chlorambucil)Mabthera (Rituximab)Mesasal (Mesalazine)MesotheliomaMethoblastin / Ledatrexate(Methotrexate)MethotrexateMitomycinMycophenolateMyleran (Busulfan)Myocrisin (Aurothiomalate)Orencia (Abatacept)Panafcort (Prednisone)Plaquenil (Hydroxychloroquine)PrednisonePuri-Nethol / 6MP (Mercaptopurine)Pyralin / Salazopyrin (Sulfasalazine)Revlimid (Lenalidomide )Rheumatrex (Methotrexate)Ridaura (Auranafin)Sandimmun (Cyclosporin)Simponi (Golimumab)TacrolimusTamoxifenTarceva (Erlotinib)Taxol (Paclitaxel)Temodal (Temozolomide)Teriflunomide (Aubagio)Thalomid (Thalidomide)Thioprine (Azathioprine)ThiotepaTilodene (Ticlopidine)Tysabri (Natalizumab)Vidaza (Azacitidine)Xeloda (Capecitabine)ZoladexFBC, ESR, CRP, BIO, MBA, EUC,LFT & if requested Gluc, Mg, CK,Chol/TrigEUCMethotrexate, Arava, Leflunomide, EnbrelEtanercept, Humira, Arabloc, CimziaFingolimod, Abatacept, TeriflunomideDialysis PatientsCyclosporin, Cicloral, CIS Platinum8

Drugs entitlement for patient having 6 visits within 6 monthsTest requestedLithiumCalcium (CA 2+ ),albuminUEC, Ca, Mg, Phos/PO 4 (CMP)Accepted drug treatmentLithium, QuilonumVitamin D or Vit D Metabolite/Analogue,Calcitrol, Rocaltrol, Citrihexal, Kosteo, Sical,Sitirol for Osteoprosis,Calcijex Denosumab XgevaCancer patient receiving biphosphonate infusionPamisol, Aredia, Bondronat, Zometa, AclastaDrugs entitlement for patient having unlimited visits within 6 monthsTest requestedINR or Prothrombin ratioAccepted drug treatmentAnticoagulant TherapyClexane, Coumadin, Dindevin, Heparin,Marevan, Warfarin, Orgaran9

Non-Medicare rebatable testsThis is a list of the most frequently requested Non-Medicare rebatable tests. Please contact (02) 9855 5400 forclarification of the fee and for details of other infrequently requested non-Medicare rebatable tests not listed here, orrefer to specific test for Medicare criteria. Patients are required to pay the amount in full, on receipt of the account(which may be from a provider, other than DHMP).TestActivated Protein C Resistance (APC Resistance)Medicare criteria:• History of venous thromboembolism OR• First degree relative who has a proven defectAdiponectinAlpha 1 Antitrypsin GenotypingAlpha Thalassaemia Gene TestAnti Diuretic Hormone (ADH) / VasopressinAnti Mullerian Hormone (AMH)Antithrombin (AT)Medicare criteria:• History of venous thromboembolism OR• First degree relative who has a proven defectApolipoprotein E GenotypingBCR–ABL FISHBCR–ABL PCRMedicare criteria:Diagnosis and monitoring of patients with laboratory evidence of:• acute myeloid leukaemia or• acute promyelocytic leukaemia or• acute lymphoid leukaemia or• chronic myeloid leukaemiaBeta 2 Adrenoreceptor Gene Test (Asthma)Beta Thalassaemia Gene TestBile Acids/Bile SaltsMedicare criteria:• patient must be pregnantBlood Pressure Monitoring (24 hour)BRAF Gene Test10

This is a list of the most frequently requested Non-Medicare rebatable tests. Please contact (02) 9855 5400 forclarification of the fee and for details of other infrequently requested non-Medicare rebatable tests not listed here, orrefer to specific test for Medicare criteria. Patients are required to pay the amount in full, on receipt of the account(which may be from a provider, other than DHMP).TestBrain Natriuretic Peptide (BNP, ProBNP)Medicare criteria:• Diagnosis of patients presenting with dyspnoea to a hospital Emergency DepartmentC1 Esterase Inhibitor Gene Test (Type I & II Hereditary Angioedema)Calprotectin – FaecesCarbohydrate Deficient Transferrin (CDT)CGH MicroarrayMedicare Criteria:• Developmental delay, intellectual disability, autism, or at least 2 congenital abnormalitiesNote: if both CGH & Chromosomes are requested on a single episode only 1 test is covered by MedicareCholinesterase GenotypingChromogranin AConnexin–26 Gene TestCystic Fibrosis Gene Test (all 32 common mutations)DAZ Gene (AZF PCR)DHEADNA Relationship TestingDrugs of Abuse Urine Testing(Non Medical such as Pre–employment, OHS, etc.)EGFR Gene Mutation (Epidermal Growth Factor Receptor)Medicare criteria:• Test of tumour tissue from a patient with locally advanced or metastatic non–small cell lung cancer requested by, or on behalfof, a specialist or consultant physician to determine if the requirements relating to epidermal growth factor receptor (EGFR) genestatus for access to gefitinib under the PBS are fulfilledEosinophil Cationic Protein (ECP)Medicare criteria:• Monitoring the response to therapy in corticosteroid treated asthma, in a child aged less than 12 yearsErythropoietin11

Non-Medicare rebatable testsThis is a list of the most frequently requested Non-Medicare rebatable tests. Please contact (02) 9855 5400 forclarification of the fee and for details of other infrequently requested non-Medicare rebatable tests not listed here, orrefer to specific test for Medicare criteria. Patients are required to pay the amount in full, on receipt of the account(which may be from a provider, other than DHMP).TestFactor V Leiden PCRMedicare criteria:• Proven DVT/PE in patient OR• Presence of mutation in first degree relativeFactor XII HAE PCR (Type III Hereditary Angioedema)Familial Hibernian Fever Gene Test (TRAPS)Familial Mediterranean Fever Gene Test (FMF)First Trimester Screen (FTS)FISH (embedded tissue specimen)FISH (excluding tissue specimens)Fluoride (Fl) – UrineFragile X PCR Gene Test (DNA Probe)Medicare criteria:• Developmental delay or family historyGanglioside AbGene Rearrangements B cellGene Rearrangements T cellGhrelinGilberts Disease Gene TestHaemochromatosis Gene Assay (GAH)Medicare criteria:• The patient has an elevated transferrin saturation or elevated serum ferritin on testing of repeated specimens, or• The patient has a first degree relative with haemochromatosis, or• The patient has a first degree relative with homozygosity for the C282Y genetic mutation, or with compound heterozygosity forrecognised genetic mutations for haemochromatosisHeat Shock Protein (HSP 70)Hepatitis C Virus (HCV) GenotypingMedicare criteria:• Pre–treatment evaluation or post treatment assessment and specialist request/advice12

This is a list of the most frequently requested Non-Medicare rebatable tests. Please contact (02) 9855 5400 forclarification of the fee and for details of other infrequently requested non-Medicare rebatable tests not listed here, orrefer to specific test for Medicare criteria. Patients are required to pay the amount in full, on receipt of the account(which may be from a provider, other than DHMP).TestHepatitis C Virus (HCV) PCR QualitativeMedicare criteria:• Patient is Hepatitis C antibody positive• Patient is Hepatitis C antibody status indeterminate• To determine Hepatitis status in immunosuppressed or immunocompromised patient• Detection of acute Hepatitis C prior to seroconversion when necessary for patient management• Patient undertaking antiviral therapy for Hepatitis CHepatitis C Virus (HCV) PCR QuantitativeMedicare criteria:• Pre–treatment evaluation or post treatment assessment and specialist request/adviceHLA B1502HLA B5701Human Epididymis Protein 4 (HE4/ROMA)Human Papilloma Virus DNA Typing (HPV)Medicare criteria:• post treatment for HSIL (CIN 2 or 3)Identification – Insect/Worm/Parasite (Referred)Inhibin BInterleukin 6Iodine – UrineISAC Microarray Allergen TestingKRAS (Kirsten ras Gene Mutation)Medicare criteria:• Test of tumour tissue from a patient with metastatic colorectal cancer requested by, or on behalf of, a specialist or consultantphysician to determine if the requirements relating to Kirsten ras (KRAS) gene mutation status for access to cetuximab under thePBS are fulfilledLeptinLipoprotein (a)Mesothelin / Mesomark13

Non-Medicare rebatable testsThis is a list of the most frequently requested Non-Medicare rebatable tests. Please contact (02) 9855 5400 forclarification of the fee and for details of other infrequently requested non-Medicare rebatable tests not listed here, orrefer to specific test for Medicare criteria. Patients are required to pay the amount in full, on receipt of the account(which may be from a provider, other than DHMP).TestMethylmalonic AcidMTHFR (Methylene Tetrahydrofolate Reductase) Gene MutationMedicare criteria:• Proven DVT/PE in patient OR• Presence of mutation in first degree relativesNon–invasive prenatal testing (NIPT) – verifiOrganochlorine insecticides – BloodOrganophosphates (Alkyl Phosphates) – UrineOsteocalcinParentage DNA TestPCA3Pharmacogenomic TestingPlatelet Ab (Pregnant)ProcalcitoninProcollagen 3 (P3NP)phi (Prostatic Health Index)Protein CMedicare criteria:• History of venous thromboembolism OR• First degree relative who has a proven defectProtein SMedicare criteria:• History of venous thromboembolism OR• First degree relative who has a proven defectProthrombin Gene Mutation (PGM)Medicare criteria:• Detection of a mutation associated with venous clottingQuantiferon Gold (Q Gold)Medicare criteria:• Immunosuppressed patient14

Drugs of AbuseUrine testingUrine Drug Screening (UDS) CategoriesUDS may be performed as a Medicare rebatable test for:• Medical assessment of patients• Monitoring of patients participating in a drug abuse treatment program(up to 36 episodes in a twelve month period)A Medicare rebate does NOT apply to the following:• Medico-legal testing• Pre-employment screening• Occupational health and safety testing• Surveillance of sports people• Testing at the request of a medical board, court of law, parole board or any similar agencySpecimen collectionMedicare rebate:No Medicare rebate:Screw-capped sterile urine container – Random collection – min vol 10mLSpecial collection kit (refer patient to specialised collection centre)Note: Specify any particular drugs of interest in clinical notes.Routine panel includes:1. Amphetamine Group: methylamphetamine, amphetamine “ecstasy” compounds, MDMA, MDA, pseudoephidrine2. Benzodiazepines: diazepam, temazepam etc.3. Cocaine metabolite4. Opiates5. Cannabinoids6. Methadone metaboliteSpecify other drugs if required such as barbiturates, alcohol, naltrexone, phenothiazines, tricyclic antidepressants.Note: Specimen collection for non-Medicare UDS is not performed at all collection centres but is limited todesignated centres where specially trained staff are available to carry out a supervised collection. If required, nonrebatablespecimens can be collected under chain-of-custody conditions and screening and confirmatory testing canbe performed under conditions specified by AS/NZS 4308: 2008. For further information about UDS and designatedcollection centres, please contact (02) 98 555 368 or your local regional laboratory.16

Pathology testsIntroductionGeneral informationThis section lists common tests and their collection requirements.The tests are listed alphabetically, by test name and abbreviation e.g. Zinc (Zn). Details for collectionrequirements are given in this section. Tests can be collected in the surgery following the collection guidelines.The specimen storage guide is located at the end of the Pathology test listing.Tests that require special tubes or specific preparation prior to arriving at the laboratory (e.g. centrifugationand freezing) are listed as “Refer patient to collection centre”. Most collection centres can do these tests,however an appointment or special instructions may be required to be given to the patient.Collection of patient specimensKey points when collecting specimens for pathology to help ensure an optimal result for your patient.Things that may affect results:• There are tubes and swabs that contain additives which may go out of date. Check that all the tubes or swabsare are in date prior to use.• Under or overfilling tubes. Check that all tubes are filled to the indicator mark.• Vaccutainer tubes are not designed for collection with a needle and syringe. Forcing the needle throughthe rubber stopper into the tube, combined with the vacuum in the tube will harm the red cells and may leadto spurious results and /or the need for a recollection.• Tipping specimens from one tube type to another. This may change the results if the specimen has begunto clot and is being tipped from a serum tube to a tube with anticoagulant, or give abnormal biochemistry ifthe specimen is contaminated with anticoagulant.Labelling requirements for pathology specimensAll specimens must be labelled with:• The patient’s given name, surname and date of birth• The date and time of collection• For a prenatal screen the signature or identifiable initials of the collector must be on the tube.If labelling requirements have not been met, the laboratory may contact the surgery to check the informationis correct. A comment will be added to the test result noting that the specimen had been incompletely, orinappropriately labelled.If the patient requires an prenatal screen, a crossmatch, or a group and hold and the request form informationand specimens do not match, or only have incomplete information, the laboratory will be unable to process thesespecimens. The patient’s blood will need to be recollected and correctly labelled.Urgent/Emergency ProtocolALL URGENT/EMERGENCY specimens need to be placed in a RED specimen bag (obtained from stores/couriers). Please indicate which test(s) is/are urgent and the required result time on the request form inthe URGENT/EMERGENCY section.To ensure these results can be phoned, faxed or downloaded to you as soon as they are available, pleaseindicate on the request form, your contact details for business hours and out-of-hours. If you require aresult within 3 hours of collection, laboratory notification is required. Please phone your laboratory withpatient details and the time the result is required. We will arrange transport of specimen. Tests such as INRs areautomatically treated as URGENT by the laboratory.17

Pathology testsAlphabetically listedTest NameCollection Requirements17 OH Progesterone PLAIN TUBE OR GEL TUBEAcetylcholine Receptor Antibody (ACHR)Acetylsalicylic AcidAcid Fast Bacilli Microscopy & Culture (AFB)- Sputum/Urine/Swab/TissueActivated Partial Thromboplastin Time(APTT)Activated Protein C Resistance (APCResistance)Adenovirus PCR - SwabAdenovirus SerologyAdiponectinAdrenal Gland AntibodyAdrenocorticotrophic Hormone (ACTH)Alanine Aminotransferase (ALT)Albumin (Alb)Alcohol - Blood (Medical)PLAIN TUBE OR GEL TUBEPLAIN TUBETreat as Urgent. Collected ASAP for toxicity.Note dosage, time of dose and collection time onreferral.YELLOW SCREW-CAPPED SPECIMENCONTAINER - EARLY MORNING SPUTUM ORFIRST MID STREAM URINE OR STUARTS SWABOR TISSUEA sputum or urine specimen should be collectedon three consecutive mornings immediately afterrising. If sputum - rinse mouth with water prior tocollection. Patient instruction sheet is available forsputum or urine collection.CITRATE TUBECitrate tube must be filled to the line at the top ofthe label (fill line) and mixed thoroughly.Refer patient to collection centreMedicare Criteria:• History of venous thromboembolism or• First degree relative who has a proven defectDRY SWABLabel swab with site of collection & test.PLAIN TUBE OR GEL TUBERefer Patient to collection centreNo Medicare rebate available.PLAIN TUBE OR GEL TUBERefer patient to collection centrePreferred collection time between 8am-10am.PLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEFLUORIDE OXALATE TUBEDo not use alcohol skin wipe. Label tube with‘Blood Alcohol’.18

Test NameAlcohol - Urine (Chain-of-Custody)Alcohol - Urine RandomAldosterone - PlasmaAldosterone - Urine 24hrAlkaline Phosphatase (ALP)Alkaline Phosphatase Isoenzymes/Fractionation (Bone/Liver) (ALP)AllopurinolAlpha 1 AntitrypsinAlpha 1 Antitrypsin - FaecesAlpha 1 Antitrypsin GenotypingAlpha 1 Antitrypsin PhenotypingAlpha Fetoprotein (AFP) (Pregnant) - SerumAlpha Fetoprotein (AFP) (Tumour Marker) -SerumCollection RequirementsRefer patient to collection centreNo Medicare rebate available.YELLOW SCREW-CAPPED SPECIMENCONTAINER - RANDOM URINEWhen requested for pre-employment, paroleboard, corporate requests or probation test isnon-Medicare rebatable.Refer patient to collection centre24 HOUR URINE CONTAINER (NILPRESERVATIVE)Where possible, patient should be off medicationfor hypertension for 2 weeks and Aldactone for atleast 6 weeks prior to the test.PLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBE4mL EDTA TUBE (separate tube required)Collect 6-9 hours post dose. Note dosage, time ofdose and collection time on referral.PLAIN TUBE OR GEL TUBERefer patient to collection centre4mL EDTA TUBE (separate tube required)No Medicare rebate available.PLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBECollect specimen between 10-17 ‘completedweeks’ gestation (ie 10-17 weeks, 6 days),although specimen can be collected up to20 weeks. For NTD (neural tube defect) riskassessment it is preferable to collect thespecimen between 15 and 17 weeks gestation.Relevant details should be included in clinicalnotes.PLAIN TUBE OR GEL TUBENote relevant clinical history on referral.19

Pathology testsAlphabetically listedTest NameAlpha Thalassaemia Gene TestAlprazolamAluminium - BloodAluminium - Urine 24 hourAluminium - Urine RandomAmikacinAmino Acids (AA) - PlasmaAmino Acids (AA) - Plasma (

Test NameAmylase/Creatinine Clearance - Urine 24hourAmylase/Creatinine Clearance - UrineRandomAndrostenedioneAngiotensinAngiotensin Converting Enzyme (ACE)Antenatal Profile includes FBC, BloodGroup, Antibody Screen, Rubella IgG,HBsAg, Hep C Ab, Syphilis Serology &UMCSAnti Diuretic Hormone (ADH) (Vasopressin)Anti Factor Xa AssayAnti Mitochondrial 2 AntibodyAnti Mullerian Hormone (AMH)Anti Neutrophilic Cytoplasmic Antibody(ANCA)Anti Nuclear Antibody (ANA)Anti Nuclear Antibody (ANA) - Synovial FluidAnti Saccharomyces Cerevisiae Antibody(ASCA)Anti Streptococcal O Titre (ASOT)Antibody ScreenAntithrombin (AT)Collection Requirements24 HOUR URINE CONTAINER (NILPRESERVATIVE) & PLAIN TUBE OR GEL TUBENote starting and finishing times on urinecontainer.YELLOW SCREW-CAPPED SPECIMENCONTAINER - MORNING URINE & PLAIN ORGEL TUBEPLAIN TUBE OR GEL TUBERefer patient to collection centrePLAIN TUBE OR GEL TUBERefer to individual tests for collection information.Refer patient to collection centreNo Medicare rebate available.Refer patient to collection centrePLAIN OR GEL TUBEPLAIN TUBE OR GEL TUBENo Medicare rebate available.PLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEYELLOW SCREW-CAPPED SPECIMENCONTAINER - SYNOVIAL FLUIDPLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBERefer patient to collection centreRefer patient to collection centreMedicare Criteria:• History of venous thromboembolism or• First degree relative who has a proven defect21

Pathology testsAlphabetically listedTest NameApolipoprotein A1Apolipoprotein BApolipoprotein E GenotypingArbovirus SerologyArsenic - BloodArsenic - Urine 24 hourArsenic - Urine RandomAspartate Transaminase (AST)Aspergillus Fumigatus IgG AntibodyAspergillus Serology (Aspergillus Precipitins& RAST-Aspergillus)Aspirate M/C/SAtypical Mycobacterium PCR - Sputum/Urine/TissueAutohaemolysis TestAvian PrecipitinsBacterial Antigen Screen - UrineBarium - Urine RandomBarmah Forest IgG/IgM AntibodyCollection RequirementsPLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBE4mL EDTA TUBE (separate tube required)No Medicare rebate available.PLAIN TUBE OR GEL TUBERefer patient to collection centreSeafood should be excluded from diet for at least5 days prior to testing.24 HOUR URINE CONTAINER (NILPRESERVATIVE)Seafood should be excluded from diet for at least5 days prior to and during testing. Note startingand finishing times on urine container.YELLOW SCREW-CAPPED SPECIMENCONTAINER - MORNING URINE (preferred)Seafood should be excluded from diet for at least5 days prior to testing.PLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEYELLOW SCREW-CAPPED SPECIMENCONTAINER - ASPIRATED BODY FLUIDYELLOW SCREW-CAPPED SPECIMENCONTAINER - EARLY MORNING SPUTUM ORFIRST MID STREAMCan also be performed on tissue.Refer patient to collection centrePLAIN TUBE OR GEL TUBEYELLOW SCREW-CAPPED SPECIMENCONTAINER - MIDSTREAM URINEGREEN SCREW-CAPPED SPECIMENCONTAINER - RANDOM URINEPLAIN TUBE OR GEL TUBE22

Test NameBCR-ABL FISHBCR-ABL PCRBCR-ABL PCR - Bone MarrowBehcets SyndromeBeta 2 Adrenoreceptor Gene Test (Asthma)Beta 2 MicroglobulinBeta 2 Microglobulin - Urine RandomBeta 2 TransferrinBeta CaroteneBeta Thalassaemia Gene TestBHCG (Beta Human ChorionicGonadotropin) (Pregnant) - SerumCollection RequirementsLITHIUM HEPARIN TUBENo Medicare rebate available. Collect Monday -Thursday only.4x 6mL EDTA TUBE - WHOLE BLOOD (separatetubes required)Must notify laboratory when collected.Medicare Criteria:• the diagnosis and monitoring of patients withlaboratory evidence of:a) acute myeloid leukaemia orb) acute promyelocytic leukaemia orc) acute lymphoid leukaemia ord) chronic myeloid leukaemiaBONE MARROW IN EDTA TUBEMust notify laboratory when collected.Medicare Criteria:• the diagnosis and monitoring of patients withlaboratory evidence of:a) acute myeloid leukaemia orb) acute promyelocytic leukaemia orc) acute lymphoid leukaemia ord) chronic myeloid leukaemia4mL EDTA TUBE (separate tube required)4mL EDTA TUBE (separate tube required)No Medicare rebate available.PLAIN TUBE OR GEL TUBEYELLOW SCREW-CAPPED SPECIMENCONTAINER - MORNING URINE (preferred)STERILE SCREW-CAPPED SPECIMENCONTAINER - NASAL OR EAR DISCHARGE (min3 drops) & PLAIN TUBE OR GEL TUBERefer patient to collection centre2x 4mL EDTA TUBE (separate tubes required)No Medicare rebate available.PLAIN TUBE OR GEL TUBE23

Pathology testsAlphabetically listedTest NameBHCG (Beta Human ChorionicGonadotropin) (Pregnant) - Urine RandomBHCG (Beta Human ChorionicGonadotropin) (Tumour Marker) - SerumBicarbonateBile Acids/SaltsBilirubinBilirubin - Body FluidBilirubin FractionatedBilirubin Neonatal / PaediatricBiopsy Tissue M/C/SBismuth - BloodBismuth - Urine 24 hourBismuth - Urine RandomBlood CultureBlood FilmBlood Gas (Arterial)Blood GroupBlood Group & Antibody ScreenCollection RequirementsYELLOW SCREW-CAPPED SPECIMENCONTAINER - FIRST MORNING URINE(preferred)PLAIN TUBE OR GEL TUBENote relevant clinical history on referral.PLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEPatient should fast for 12 hours prior to test. IfURGENT please notify the laboratory.Medicare Criteria:• patient must be pregnant to receive rebatePLAIN TUBE OR GEL TUBEYELLOW SCREW-CAPPED SPECIMENCONTAINER - BODY FLUID OR VOMITUS ORASPIRATEPLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBE (PAEDIATRIC)Treat as URGENT.YELLOW SCREW-CAPPED SPECIMENCONTAINER - BIOPSY TISSUE (FRESH)4mL EDTA TUBE24 HOUR URINE CONTAINER (NILPRESERVATIVE)Note starting and finishing times on urinecontainer.YELLOW SCREW-CAPPED SPECIMENCONTAINER - MORNING URINE (preferred)BLOOD CULTURE BOTTLES4mL EDTA TUBEContact local laboratory for information.Refer patient to collection centreRefer patient to collection centre24

Test NameBlood Pressure Monitoring (24 hour)Bone Marrow ExaminationBordetella Pertussis IgA/IgG AntibodyBordetella Pertussis PCR - NasopharyngealAspirateCollection RequirementsRefer patient to collection centreNo Medicare rebate available.Contact local laboratory for information.PLAIN TUBE OR GEL TUBENASOPHARYNGEAL ASPIRATEBordetella Pertussis PCR - Swab DRY SWAB OR BACTERIAL SWAB (BLUE) -THROAT OR NASOPHARYNXThe preferred collection is 1x DRY swab for eachPCR test except when Chlamydia requested withGonorrhoeae. Label swab with site of collection& test.Brain Natriuretic Peptide (NT-Pro BNP)Bronchial Washings CytologyBroncho-Alveolar Lavage M/C/SBrucella AntibodyBrushing Smear CytologyC difficile Toxin PCR - FaecesC PeptideC Reactive Protein (CRP)C-TelopeptideC1 Esterase InhibitorPLAIN TUBE OR GEL TUBEMedicare criteria:• rebate only available for diagnosis of patientspresenting with dyspnoea to a hospitalEmergency DepartmentFLUID &/or SLIDE OR BAL BOTTLESFLUID &/or SLIDE OR BAL BOTTLESPLAIN TUBE OR GEL TUBECYTOLOGY SMEARLabel slide clearly in pencil with patient name anddate of birth, type of specimen and site. Preparesmear and fix with spray fixative or immersein 95% alcohol for 20 minutes. Allow to air drycompletely before placing in slide container.BROWN TOP CONTAINER - FAECESRefer patient to collection centrePatient should be fasting 8 hours.PLAIN TUBE OR GEL TUBERefer patient to collection centrePatient should be fasting 8 hoursRefer patient to collection centre25

Pathology testsAlphabetically listedTest NameC1 Esterase Inhibitor Gene TestCA 125CA 15-3CA 19-9CA 72-4Cadmium - BloodCadmium - Urine 24 hourCadmium - Urine RandomCaeruloplasminCalcitoninCalciumCalcium - IonisedCalcium - Urine 24 hourCalcium (Stasis Free)Calcium Creatinine Ratio - Urine RandomCalculi/CalculusCandida AntibodyCollection Requirements4mL EDTA TUBE (separate tube required)No Medicare rebate available.PLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBERefer patient to collection centre24 HOUR URINE CONTAINER (NILPRESERVATIVE)Note starting and finishing times on urinecontainer.YELLOW SCREW-CAPPED SPECIMENCONTAINER - MORNING URINE (preferred)PLAIN TUBE OR GEL TUBERefer patient to collection centrePLAIN TUBE OR GEL TUBEIf stasis free (ie no tourniquet) note on referral.PLAIN TUBE OR GEL TUBE (separate tuberequired)Fasting is preferred and collected stasis free.24 HOUR URINE CONTAINER (NIL OR HCLPRESERVATIVE)Note starting and finishing times on urinecontainer.PLAIN TUBE OR GEL TUBECollect without the use of a tourniquet.Refer patient to collection centreYELLOW SCREW-CAPPED SPECIMENCONTAINER - RENAL CALCULI (STONE)PLAIN TUBE OR GEL TUBE26

Test NameCarbamazepineCarbohydrate Deficient Transferrin (CDT)CarboxyhaemoglobinCarcinoembryonic Antigen (CEA)Cardiac Isoenzymes (CK-MB)Cardiac Markers inc. Troponin TCardiolipin Antibody inc Beta 2 GlycoproteinAntibody (ACL)Cat Scratch SerologyCatecholamines (CATS) - PlasmaCatecholamines (CATS) - Urine 24 hourCatheter Tip M/C/SCerebrospinal Fluid CytologyCerebrospinal Fluid M/C/SCGH MicroarrayCollection RequirementsPLAIN TUBE OR GEL TUBECollect just before next dose or at least 6 hourspost dose. Note dosage, time of dose andcollection time on referral.PLAIN TUBE OR GEL TUBENo Medicare rebate available.4mL EDTA TUBETreat as URGENT if poisoning suspected.PLAIN TUBE OR GEL TUBENote relevant clinical history on referral.PLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBETreat as URGENTPLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBERefer patient to collection centre24 HOUR URINE CONTAINER (HCLPRESERVATIVE)Note starting and finishing times on urinecontainer.YELLOW SCREW-CAPPED SPECIMENCONTAINER - TIPSTERILE SCREW-CAPPED SPECIMENCONTAINER - CSFTransport to the laboratory without delay.STERILE SCREW-CAPPED SPECIMENCONTAINER - CSFEDTA TUBE - WHOLE BLOOD & LITHIUMHEPARIN TUBE - WHOLE BLOOD (separatetubes required)Note clinical history on referral form.Medicare rebatable for only 1 test if bothChromosomes & CGH Microarray requested.27

Pathology testsAlphabetically listedTest NameChemistry - Gastric FluidChlamydia trachomatis AntibodyCollection RequirementsSTERILE SCREW-CAPPED SPECIMENCONTAINER - GASTRIC FLUIDPLAIN TUBE OR GEL TUBEChlamydia trachomatis PCR - Swab DRY SWAB OR BACTERIAL SWAB (BLUE) -CERVICAL OR URETHRALThe preferred collection is 1x DRY swab for eachPCR test except when Chlamydia requested withGonorrhoeae. Label swab with site of collection& test.Chlamydia trachomatis PCR - ThinPrepChlamydia trachomatis PCR - Urine FirstVoidChlamydophilia IgG/IgA AntibodyChlorideChloride - CSFChloride - Urine 24 hourChloride - Urine RandomChlorpromazineCholesterolCholinesteraseCholinesterase - PlasmaCholinesterase - Red CellCholinesterase - Serum and Red CellTHINPREP VIALYELLOW SCREW-CAPPED SPECIMENCONTAINER - FIRST VOID URINECollect the first 20-30mL of the urine stream.PLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBESTERILE SCREW-CAPPED SPECIMENCONTAINER - CSF24 HOUR URINE CONTAINER (NILPRESERVATIVE)Note starting and finishing times on urinecontainer.YELLOW SCREW-CAPPED SPECIMENCONTAINER - MORNING URINENote time of collection on jar.PLAIN TUBECollect just before next dose. Note dosage, timeof dose and collection time on referral.PLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBELITHIUM HEPARIN TUBELITHIUM HEPARIN TUBECollect Monday to Thursday only.PLAIN TUBE OR GEL TUBE & LITHIUM HEPARINTUBECollect Monday to Thursday only.28

Test NameCholinesterase GenotypingChromatin AntibodyChromiumChromium - Body FluidChromium - Urine 24 hourChromium - Urine RandomChromogranin AChromosome Analysis / Studies - BloodChromosome Analysis / Studies - BoneMarrowChromosome Analysis / Studies - FreshTissue or POCCitrate - Urine 24 hourCitrate - Urine RandomClomipramineClonazepamCollection RequirementsRefer patient to collection centreNo Medicare rebate available.PLAIN TUBE OR GEL TUBERefer patient to collection centreSTERILE SCREW-CAPPED SPECIMENCONTAINER - BODY FLUID24 HOUR URINE CONTAINER (NILPRESERVATIVE)Note starting and finishing times on urinecontainer.YELLOW SCREW-CAPPED SPECIMENCONTAINER - MORNING URINE (preferred)Refer patient to collection centreNo Medicare rebate available.LITHIUM HEPARIN TUBEMedicare rebatable for only 1 test if bothChromosomes & CGH Microarray requested.BONE MARROW IN HANKS SOLUTIONFRESH TISSUE OR POC IN STERILE SALINESOLUTION24 HOUR URINE CONTAINER (NILPRESERVATIVE)Note starting and finishing times on urinecontainer.YELLOW SCREW-CAPPED SPECIMENCONTAINER - MORNING URINENote time of collection on jar.PLAIN TUBECollect just before next dose. Note dosage, timeof dose and collection time on referral.LITHIUM HEPARIN TUBECollect just before next dose. Note dosage, timeof dose and collection time on referral.29

Pathology testsAlphabetically listedTest NameClozapineCoagulation ScreenCobalt - PlasmaCobalt - Urine 24 hourCobalt - Urine RandomCodeineCoeliac Serology (Gliadin & TTG) AbCoeliac Tissue TypingCold AgglutininsComplement C1QComplement C2Complement C3Complement C3 C4Complement C4Complement C5Complement C6Complement C7, C8 or C9Complement Total (CH50/CH100)Collection RequirementsLITHIUM HEPARIN TUBECollect just before next dose. Note dosage, timeof dose and collection time on referral.2x CITRATE TUBE & 4mL EDTA TUBETreat as Urgent. Citrate tube must be filled tothe line at the top of the label (fill line) and mixedthoroughly.Refer patient to collection centre24 HOUR URINE CONTAINER (NILPRESERVATIVE)Note starting and finishing times on urinecontainer.YELLOW SCREW-CAPPED SPECIMENCONTAINER - RANDOM URINEFor occupational exposure, urine should becollected at end of shift. Label jar with time anddate of collection.PLAIN TUBEFor toxicity collect as soon as possible and treatas Urgent.PLAIN TUBE OR GEL TUBE4mL EDTA TUBE (separate tube required)Refer patient to collection centrePLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBERefer patient to collection centreRefer patient to collection centreRefer patient to collection centre30

Test NameConnexin-26 Gene TestCopper - PlasmaCopper - SerumCopper - Urine 24 hourCopper - Urine RandomCortisolCortisol - Urine 24 hourCortisol - Urine RandomCotinine - SerumCotinine - Urine RandomCreatine Kinase (CK)CreatinineCreatinine - Urine 24 hourCreatinine - Urine RandomCollection Requirements4mL EDTA TUBE (separate tube required)No Medicare rebate available.Special consent paperwork will be followed up byreferral laboratory before test can be performed.Refer patient to collection centrePLAIN TUBE OR GEL TUBE24 HOUR URINE CONTAINER (NILPRESERVATIVE)Note starting and finishing times on urinecontainer.YELLOW SCREW-CAPPED SPECIMENCONTAINER - MORNING URINE (preferred)PLAIN TUBE OR GEL TUBENote collection time & any hormone therapy onreferral.24 HOUR URINE CONTAINER (NILPRESERVATIVE)Note starting and finishing times on urinecontainer.YELLOW SCREW-CAPPED SPECIMENCONTAINER - MORNING URINENote time of collection on jar.PLAIN TUBE OR GEL TUBEYELLOW SCREW-CAPPED SPECIMENCONTAINER - RANDOM URINEPLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBE24 HOUR URINE CONTAINER (ANYPRESERVATIVE)Note starting and finishing times on urinecontainer.YELLOW SCREW-CAPPED SPECIMENCONTAINER - MORNING URINENote time of collection on jar.31

Pathology testsAlphabetically listed32Test NameCreatinine Clearance - Urine 24 hourCrossmatch (Group & Hold)CryofibrinogenCryoglobulinsCryptococcus AntigenCryptococcus Antigen - CSFCyclic Citrullinated Peptide Antibody (CCP)CyclosporinCystic Fibrosis (all 32 Common Mutations)Cystine - Urine 24 hourCystine - Urine RandomCytomegalovirus IgG/IgM Antibody (CMV)D Dimer (Fibrinogen Degradation Products)DAZ Gene PCR (AZF PCR)Dehydroepiandrosterone (DHEA)Dehydroepiandrosterone Sulphate (DHEAS)Collection Requirements24 HOUR URINE CONTAINER (NIL OR ANYPRESERVATIVE) & PLAIN TUBE OR GEL TUBENote starting and finishing times on urinecontainer.Refer patient to collection centreCITRATE TUBECitrate tube must be filled to the line at the top ofthe label (fill line) and mixed thoroughly.Refer patient to collection centrePLAIN TUBE OR GEL TUBESTERILE SCREW-CAPPED SPECIMENCONTAINER - CSFPLAIN TUBE OR GEL TUBE4mL EDTA TUBE (separate tube preferred)Collect just before next dose or 2 hours postdose. Note dosage, time of dose and collectiontime on referral.4mL EDTA TUBE (separate tube required)No Medicare rebate available. Note any familyhistory of Cystic Fibrosis and mutations if known.24 HOUR URINE CONTAINER (NILPRESERVATIVE)Note starting and finishing times on urinecontainer.YELLOW SCREW-CAPPED SPECIMENCONTAINER - MORNING URINE (preferred)PLAIN TUBE OR GEL TUBECITRATE TUBETreat as Urgent. Citrate tube must be filled tothe line at the top of the label (fill line) and mixedthoroughly. Clinical history of patient required.4mL EDTA TUBE (separate tube required)No Medicare rebate available.PLAIN TUBE OR GEL TUBENo Medicare rebate available.PLAIN TUBE OR GEL TUBE

Test NameDengue Fever IgG/IgM AntibodyDeoxypyridinoline Cross Links (DPD X-Links)Dexamethasone Suppression TestDiazepamDigoxinDiphtheria AntibodyDirect CoombsDisaccharidase Assay (DSAC) - TissueDisseminated Intravascular CoagulationScreenDNA Relationship TestingDNAse BDopamine - Urine 24 hourDouble stranded DNA Antibody (dsDNA)Collection RequirementsPLAIN TUBE OR GEL TUBEYELLOW SCREW-CAPPED SPECIMENCONTAINER - MORNING URINE (FIRST ORSECOND VOID)Note time of collection on jar.Refer patient to collection centrePLAIN TUBECollect just before next dose. Note dosage, timeof dose and collection time on referral.PLAIN TUBECollect just before next dose or at least 6 hoursbut preferably 8-10 hours post dose. Notedosage, time of dose and collection time onreferral.PLAIN TUBE OR GEL TUBERefer patient to collection centreSTERILE SCREW-CAPPED SPECIMENCONTAINER - BIOPSY TISSUEIdeally 2x specimens must be wrapped separatelyin foil or parafilm to avoid dehydration then placedin sterile container. Freeze as soon as possible.4mL EDTA TUBE & CITRATE TUBETreat as Urgent. Citrate tube must be filled tothe line at the top of the label (fill line) and mixedthoroughly.Refer patient to collection centreNo Medicare rebate available. Contact DNALabson 1300 663 244 for further information.PLAIN TUBE OR GEL TUBE24 HOUR URINE CONTAINER (HCLPRESERVATIVE)Note starting and finishing times on urinecontainer.PLAIN TUBE OR GEL TUBE33

Pathology testsAlphabetically listedTest NameDowns Syndrome Screen (Second Trimester)DoxepinDrug Screen (Legal) - BloodDrugs of Abuse Urine Testing (Chain-of-Custody)Drugs of Abuse Urine Testing (Medical)Drugs of Abuse Urine Testing (MethadoneClinic) - Urine RandomE/LFT (Electrolytes/LFT)EGF Receptor Gene mutation (EpidermalGrowth Factor)Electrocardiogram (ECG)ElectrolytesElectrolytes - Urine 24 hourElectrolytes - Urine RandomEndomysial IgA AntibodyEndoscope CultureCollection RequirementsPLAIN TUBE OR GEL TUBECollect specimen between 15-17 completedweeks gestation (ie. 17 weeks, 6 days). Noterelevant details on referral eg LMP, ultrasounddata.PLAIN TUBECollect just before next dose. Note dosage, timeof dose and collection time on referral.Test not available through DHM Pathology.Refer patient to collection centreNo Medicare rebate available.YELLOW SCREW-CAPPED SPECIMENCONTAINER - RANDOM URINEWhen requested for pre-employment, paroleboard, corporate requests or probation test isnon-Medicare rebatable.GREEN SCREW-CAPPED SPECIMENCONTAINER - RANDOM URINEPLAIN TUBE OR GEL TUBEFRESH TISSUE OR PARRAFIN EMBEDDEDTISSUEContact laboratory for information.Refer patient to collection centrePartial Medicare Rebate.PLAIN TUBE OR GEL TUBE24 HOUR URINE CONTAINER (NILPRESERVATIVE)Note starting and finishing times on urinecontainer.YELLOW SCREW-CAPPED SPECIMENCONTAINER - MORNING URINENote time of collection on jar.PLAIN TUBE OR GEL TUBESTERILE SCREW-CAPPED SPECIMENCONTAINER - ENDOSCOPE WASHINGSNo Medicare rebate available.34

Test NameEnterovirus AntibodyEnterovirus PCR - SwabEosinophil Cationic Protein (ECP)Epstein Barr Virus Early Antigen (EBVEA)Epstein Barr Virus IgG/IgM Antibody (EBV)Epstein Barr Virus Viral Capsid Antigen IgA(EBVA)Erythrocyte Sedimentation Rate (ESR)ErythropoietinEthosuximideEUC (Electrolytes, Urea & Creatinine)EverolimusExtractable Nuclear Antigen (ENA)Factor IIFactor IXFactor IX InhibitorFactor VFactor V Leiden PCRFactor VIICollection RequirementsPLAIN TUBE OR GEL TUBE2x DRY SWABS - NOSE & THROATPLAIN TUBE OR GEL TUBEMedicare Criteria:• Monitoring the response to therapy incorticosteroid treated asthma, in a child agedless than 12 years.PLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEClinical notes must indicate ‘investigation ofNasopharyngeal Carcinoma’ for test to beperformed.4mL EDTA TUBEPLAIN TUBE OR GEL TUBENo Medicare rebate available.PLAIN TUBECollect just before next dose. Note dosage, timeof dose and collection time on referral.PLAIN TUBE OR GEL TUBE4mL EDTA TUBEPLAIN TUBE OR GEL TUBERefer patient to collection centreRefer patient to collection centreRefer patient to collection centreRefer patient to collection centre4mL EDTA TUBE (separate tube required)Medicare Criteria:• proven DVT/PE (deep vein thrombosis/pulmonary embolism) in patient or• presence of mutation in first degree relativeRefer patient to collection centre35

Pathology testsAlphabetically listedTest NameFactor VIII InhibitorFactor VIII:C AssayFactor XFactor XIFactor XIIFactor XIIIFaecal Fat (3 Day)Faeces Occult Blood x3Faeces OCP & CultureFamilial Mediterranean Fever Gene Test(FMF)Farmers Lung PrecipitinsFasciola SerologyFerritinFibrinogenFilarial ParasitesFilariasis SerologyCollection RequirementsRefer patient to collection centreRefer patient to collection centreRefer patient to collection centreRefer patient to collection centreRefer patient to collection centreRefer patient to collection centreRefer patient to collection centreFAECAL OCCULT BLOOD KITCollect 3 specimens from 3 separate motions.BROWN TOP CONTAINER - FAECESIf Dientamoeba Fragilis is required, use the specialSAF collection kit available from Douglass HanlyMoir Stores department (02) 9855 5210.4mL EDTA TUBE (separate tube required)No Medicare rebate available.PLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBECITRATE TUBECitrate tube must be filled to the line at the top ofthe label (fill line) and mixed thoroughly.4mL EDTA TUBEPLAIN TUBE OR GEL TUBE36

Test NameFine Needle Aspiration (FNA)Fine Needle Aspiration (FNA) (pathologistcollection)First Trimester Screen (FTS/PAPPA)FISH (Chromosome) - BloodFISH (embedded tissue specimen)Flow Cytometry - FNAFluid CytologyCollection RequirementsAIR DRIED AND/OR FIXED SMEARS, NEEDLERINSE +/- STERILE CONTAINER - NON GYNAEFLUIDLabel slides clearly in pencil with patient name,date of birth specimen type and site. Makesmears directly from needle contents. Quicklyspray fix half of the slides and air dry theremaining slides. Please mark slides as A/D(air dried) or W/F (wet fixed). The needle maybe rinsed into a clearly labelled 5mL containercontaining Hanks Balanced Salt Solution(available from the laboratory). Please DO NOTforward needles to the laboratory. If there is alarger volume of fluid place in a clearly labelledsterile container. DO NOT add fixative to the fluid.Contact local laboratory for booking or enquiries.PLAIN TUBE OR GEL TUBEPartial Medicare RebateCollect specimen between 10-13 completedweeks gestation (ie. 13 weeks, 6 days). Noterelevant details on referral eg LMP, ultrasounddata.LITHIUM HEPARIN TUBENo Medicare rebate available.Collect Monday - Thursday only.Contact Histopathologist for all enquiries.FNA IN HANKS SOLUTIONSTERILE CONTAINER - FLUIDLabel container clearly with patient name, dateof birth, specimen type and site. If the specimenis very scanty (i.e. several drops only) slides canbe made directly from the fluid. Slides should beclearly labelled in pencil with patient name, dateof birth, specimen type and site. Alternatively forsmall fluid volumes add a small amount of normalsaline to the specimen to avoid dehydration. DONOT add fixative to the fluid.37

Pathology testsAlphabetically listedTest NameFluoride - Urine RandomFolate - Red Cell (RCF) (preferred)Follicle Stimulating Hormone (FSH)Fragile X PCR Gene Test (DNA Probe)Free Light Chain Typing - SerumFree TestosteroneFree Thyroxine (FT4)Free Triiodothyronine (FT3)Collection RequirementsYELLOW SCREW-CAPPED SPECIMENCONTAINER - RANDOM URINENo Medicare rebate available.Collect at end of shift or following exposureMonday to Thursday only.4mL EDTA TUBEPLAIN TUBE OR GEL TUBEIf female, include LMP & any exogenous hormonetherapy on referral.4mL EDTA TUBE (separate tube required)Medicare Criteria:• developmental delay or• patient exhibits clinical features of FRXsyndrome or• family history of Fragile X mutationPLAIN TUBE OR GEL TUBECalculated from serum testosterone, albumin &SHBG results.PLAIN TUBE OR GEL TUBERelevant clinical notes required for test to beperformed• TSH is abnormal or• monitoring thyroid disease or• psychiatric investigation or dementia or• infertility investigation or amenorrhoea or• pituitary dysfunction suspected or• on drugs interfering with thyroid function or• investigating sick euthyroid syndrome inadmitted patientPLAIN TUBE OR GEL TUBERelevant clinical notes required for test to beperformed• TSH is abnormal or• monitoring thyroid disease or• psychiatric investigation or dementia or• infertility investigation or amenorrhoea or• pituitary dysfunction suspected or• on drugs interfering with thyroid function or• investigating sick euthyroid syndrome inadmitted patient38

Test NameFructosamineFructose - SemenFull Blood Count (FBC)Fungal Microscopy & CultureFungal PrecipitinsGabapentinGalactokinaseGamma Glutamyl Transferase (GGT)Ganglioside AntibodiesGastric Parietal Cell Antibody (GPCA)GastrinGene Rearrangements T & B CellGentamicinGhrelinGilberts Disease Gene TestGlomerular Basement Membrane Antibody(GBM)Glucagon - PlasmaGlucose - Joint/Synovial FluidGlucose - Plasma (Blood Sugar Level, BSL)Collection RequirementsPLAIN TUBE OR GEL TUBERefer patient to collection centre4mL EDTA TUBEBLACK BOX CONTAINER - SKIN SCRAPINGSOR NAIL CLIPPINGSPLAIN TUBE OR GEL TUBEPLAIN TUBECollect just before next dose. Note dosage, timeof dose and collection time on referral.Refer patient to collection centrePLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEPartial Medicare Rebate.PLAIN TUBE OR GEL TUBERefer patient to collection centre4mL EDTA TUBE (separate tube required) ORBONE MARROW/TISSUE/FNANo Medicare rebate available.PLAIN TUBECollect just before next dose. Peak level iscollected 1/2 to 1 hours post dose. Note dosage,time of dose and collection time on referral.Refer patient to collection centreNo Medicare rebate available.4mL EDTA TUBE (separate tube required)No Medicare rebate available.PLAIN TUBE OR GEL TUBERefer patient to collection centreSTERILE SCREW-CAPPED SPECIMENCONTAINER - BODY FLUIDFLUORIDE OXALATE TUBENote if fasting or random collection.39

Pathology testsAlphabetically listedTest NameGlucose - Plasma (Blood Sugar Level, BSL)(2hr Post Prandial)Glucose - Serum (Blood Sugar Level, BSL)Glucose - Urine 24 hourGlucose - Urine RandomGlucose 6 Phosphate Dehydrogenase(G6PD)Glucose Challenge Test (GCT) - 50g load(Pregnant)Glucose Tolerance Test (GTT) - 2hr 75g (NonPregnant)Glucose Tolerance Test (GTT) - 2hr 75g(Pregnant)Glucose Tolerance Test with Insulins(INSGTT)Glutamic Acid Decarboxylase Antibody(GAD)Collection RequirementsFLUORIDE OXALATE TUBECollect 2 hours after a meal AM or PM. Label tubeas ‘post-prandial’ & time of collection.PLAIN TUBE OR GEL TUBENote if fasting or random collection.24 HOUR URINE CONTAINER (NILPRESERVATIVE)Note starting and finishing times on urinecontainer.YELLOW SCREW-CAPPED SPECIMENCONTAINER - RANDOM URINE4mL EDTA TUBERefer patient to collection centreRefer patient to collection centreRefer patient to collection centreRefer patient to collection centrePLAIN TUBE OR GEL TUBEGonorrhoeae PCR - Swab DRY SWAB OR BACTERIAL SWAB (BLUE) -CERVICAL OR URETHRALThe preferred collection is 1x DRY swab for eachPCR test except when Chlamydia requested withGonorrhoeae. Label swab with site of collection& test.Gonorrhoeae PCR - Thin PrepGonorrhoeae PCR - Urine First VoidTHINPREP VIALYELLOW SCREW-CAPPED SPECIMENCONTAINER - FIRST VOID URINECollect the first 20-30mL of the urine stream.40

Test NameGroup B Streptococcus PCRGrowth Hormone (GH)Growth Hormone Stimulation Test withResponse to ExerciseGrowth Hormone Suppression Test withResponse to GlucoseGuthrie TestHaematocrit (HCT)Haemochromatosis Gene Assay (GAH)Haemoglobin (Hb)Haemoglobin A1c (GHB)Haemolysis ScreenHaemophilus Influenza Type B AntibodyHaemosiderin - Urine RandomHaptoglobinHeat Shock Protein (HSP 70)Collection RequirementsDRY SWAB OR BACTERIAL SWAB (BLUE)The preferred collection is 1x DRY swab for eachPCR test requested (except Chlamydia/Gono).Label swab with site of collection & test.PLAIN TUBE OR GEL TUBERefer patient to collection centreRefer patient to collection centreGUTHRIE CARD - DRIED BLOOD SPOT(Collected by heal-prick)4mL EDTA TUBE4mL EDTA TUBE (separate tube required)Medicare criteria:• the patient has an elevated transferrinsaturation or elevated serum ferritin on testingof repeated specimens or• the patient has a first degree relative withhaemochromatosis or• the patient has a first degree relative withhomozygosity for the C282Y geneticmutation, or with compound heterozygosityfor recognised genetic mutations forhaemochromatosis4mL EDTA TUBE4mL EDTA TUBE4mL & 6mL EDTA TUBES & PLAIN TUBE OR GELTUBEPLAIN TUBE OR GEL TUBEYELLOW SCREW-CAPPED SPECIMENCONTAINER - MORNING URINE (preferred)PLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBENo Medicare rebate available.41

Pathology testsAlphabetically listed42Test NameHeavy Metal Screen (Arsenic, Lead,Cadmium & Mercury)Heavy Metal Screen (Arsenic, Lead,Cadmium & Mercury) - Urine 24 hourHeavy Metal Screen (Arsenic, Lead,Cadmium & Mercury) - Urine RandomHeinz BodiesHelicobacter Pylori Faecal AntigenHelicobacter Pylori IgG AntibodyHeparin Induced Platelet Antibodies (HITTS)Hepatitis A & B (Immunity)Hepatitis A IgG (HAVIgG) (Immunity)Hepatitis A IgM (HAVIgM) (Acute)Hepatitis A,B,C (Acute)Hepatitis B (HBcAb) core AntibodyHepatitis B (HBcIgM) core IgMHepatitis B (HBeAb) e AntibodyHepatitis B (HBeAg) e Antigen (Carrierstatus)Hepatitis B (HBsAb) surface Antibody(Immunity)Hepatitis B (HBsAg) surface Antigen (Acute)Collection RequirementsRefer patient to collection centreSeafood should be excluded from diet for at least5 days prior to testing. Note specific metals onreferral.24 HOUR URINE CONTAINER (NILPRESERVATIVE)Seafood should be excluded from diet for at least5 days prior to and during testing. Note specificmetals on referral. Note starting and finishingtimes on urine container.YELLOW SCREW-CAPPED SPECIMENCONTAINER - MORNING URINE (preferred)Seafood should be excluded from diet for at least5 days prior to testing. Note specific metals onreferral.4mL EDTA TUBEBROWN TOP CONTAINER - FAECESPLAIN TUBE OR GEL TUBEPLAIN TUBEInformation of heparin given or preferably anampoule/vial given to patient prior to test shouldaccompany this request. Record how long thepatient has been on heparin on the referral.PLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBE

Test NameHepatitis B delta AntibodyHepatitis B DNA (Viral Load)Hepatitis C Antibody (HCV)Hepatitis C Genotyping (HCV Genotyping)Hepatitis C PCR Qualitative (HCV PCR Qual)Hepatitis C PCR Quantitative (HCV PCR ViralLoad)Hereditary Angioedema Type III (Factor XIIMutation)Hereditary SphereocytosisHerpes & Varicella PCR - SwabCollection RequirementsPLAIN TUBE OR GEL TUBEMedicare Criteria:• Must be Hepatitis B positiveRefer patient to collection centreMedicare Criteria:• Must be Hepatitis B positivePLAIN TUBE OR GEL TUBERefer patient to collection centreMedicare Criteria:• Pre-treatment evaluation or post treatmentassessment and specialist request/adviceRefer patient to collection centreMedicare Criteria:• Patient is Hepatitis C antibody positive or• Patient is Hepatitis C antibody statusindeterminate or• To determine Hepatitis status inimmunosuppressed or immunocompromisedpatient or• Detection of acute Hepatitis C prior toseroconversion when necessary for patientmanagement or• Patient undertaking antiviral therapy forHepatitis CRefer patient to collection centreMedicare Criteria:• Pre-treatment evaluation for antiviral therapyfor chronic Hep C and test advised byspecialist who manages treatment of thepatients hepatitis• 12 week assessment on combination antiviraltreatment4mL EDTA TUBE (separate tube required)No Medicare rebate available.4mL EDTA TUBE (separate tube required)Collect Monday to Thursday only.DRY SWAB OR BACTERIAL SWAB (BLUE)The preferred collection is 1x DRY swab for eachPCR test except when Chlamydia requested withGonorrhoeae. Label swab with site of collection& test.43

Pathology testsAlphabetically listedTest NameHerpes Simplex Virus Antibody (HSV)Heterophile AntibodyHigh Density Lipoprotein (HDL)Histamine - BloodHistamine - Urine 24 hourHistone AntibodyHistopathologyHistopathology - Frozen SectionHistopathology - GynaepathHistopathology - ImmunofluorescenceHistoplasma AntibodyHIV 1/2 Antigen & AntibodyHIV Drug Resistant GenotypeHIV Viral LoadCollection RequirementsPLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEFasting 8-12 hours recommended. Note on formif fasting and if patient is on any lipid loweringdrugs. Lipid requests will only include Chol/Trig ifHDL required test must be specifically requested.Refer patient to collection centre24 HOUR URINE CONTAINER (NILPRESERVATIVE)Note starting and finishing times on urinecontainer. Histamine diet to be followed 24 hoursprior to and for the duration of the test.PLAIN TUBE OR GEL TUBETISSUE IN FORMALINPlace in 10% buffered formalin. Ideally the volumeof fixative should be at least 10 times that of thespecimen.TISSUE - FROZEN SECTIONContact local laboratory for booking.TISSUE IN FORMALINBIOPSY (ENDOMETRIAL, CERVICAL, CORE,VULVAL), UTERUS, OVARY, FALLOPIAN TUBESOR FIBROIDSDo NOT place in fixative. Place in transportmedium or wrap in gauze moistened with normalsaline in sterile container. Transport to thelaboratory without delay. Place any accompanyingspecimen for routine histopathology in formalinas usual.PLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBERefer patient to collection centreMedicare Criteria:• max 2 tests per yearRefer patient to collection centre44

Test NameHLA B1502HLA B27HLA B5701HLA Tissue Typing - First visit (Red Cross)HLA Tissue Typing - Monthly (Red Cross)Holotranscobalamin Active B12Holter Monitor 24 hourHoma IndexHomocysteine - PlasmaHomocystine - Urine RandomHomogentisic Acid - Urine RandomHomovanillic Acid (HVA) - Urine 24 hourHoney Bee Venom IgGHTLV I & II AntibodyHuman Epididymis Protein 4 (HE4/ROMA)Human Herpes Virus Type 6 (HHV6)Collection Requirements4mL EDTA TUBE (separate tube required)No Medicare rebate available.4mL EDTA TUBE (separate tube required)4mL EDTA TUBE (separate tube required)Partial Medicare Rebate.Refer patient to collection centreNo Medicare rebate available (except transplantpatients).Refer patient to collection centreNo Medicare rebate available (except transplantpatients).PLAIN TUBE OR GEL TUBERefer patient to collection centrePLAIN TUBE OR GEL TUBEPatient must be fastingRefer patient to collection centrePatient must be fasting 8-12 hours.YELLOW SCREW-CAPPED SPECIMENCONTAINER - MORNING URINE (preferred)Refer patient to collection centre24 HOUR URINE CONTAINER (HCLPRESERVATIVE)Note starting and finishing times on urinecontainer.PLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBENo Medicare rebate available.PLAIN TUBE OR GEL TUBE45

Pathology testsAlphabetically listedTest NameHuman Papilloma Virus DNA Typing (HPV)Hydatid AntibodyHydroxyindoleacetic Acid (5HIAA) - Urine 24hourHydroxymethoxymandelic Acid (HMMA) -Urine 24 hourHydroxyproline - Urine RandomHydroxypyreneCollection RequirementsTHINPREP VIALMedicare Criteria:• post treatment for HSIL (CIN 2 or 3)1) Co-collection with the conventional Papsmear and the ThinPrep test◦{The conventional Pap smear is performedand the sampling device is then rinsedvigorously in the ThinPrep vial.◦{The HPV test can be performed from thematerial remaining in the vial after theThinPrep test has been completed.There is no need to take a separatesample for the HPV test.2) Co-collection with the conventional Papsmear◦{The conventional Pap smear is performedfirst.◦{Then sampling device is then rinsedvigorously in the ThinPrep vial.◦{Please clearly indicate that the ThinPrepvial is for HPV testing only.3) As a separate stand-alone specimen◦{The HPV test is collected with a cervicalcytology sampler which is rinsedvigorously in the ThinPrep vial.◦{Please clearly indicate that the ThinPrepvial is for HPV testing only.The HPV test can also be performed onsolid tissue biopsies (eg. anal papillomas)PLAIN TUBE OR GEL TUBE24 HOUR URINE CONTAINER (HCLPRESERVATIVE)Note starting and finishing times on urinecontainer. Patient to follow 5HIAA/5HT (Serotonin)dietary advice sheet.24 HOUR URINE CONTAINER (HCLPRESERVATIVE)Note starting and finishing times on urinecontainer.Refer patient to collection centreRefer patient to collection centreNo Medicare rebate available.46

Test NameIdentification - Insect/Worm/ParasiteImipramine & DesipramineImmunoglobulin A (IgA)Immunoglobulin D (IgD)Immunoglobulin E (IgE)Immunoglobulin G (IgG)Immunoglobulin G SubclassesImmunoglobulin M (IgM)Immunoglobulins GAMImuran MetabolitesInfluenza AntibodyInfluenza PCRInhibin BInsect IdentificationInsulinInsulin AntibodyInsulin Like Growth Factor 1 (IGF1)Collection RequirementsYELLOW SCREW-CAPPED SPECIMENCONTAINER - WORM/INSECT/PARASITEIf DHM laboratory are unable to report, thespecimen can be forwarded to ICPMR. If sent toICPMR test is non-Medicare rebatable.PLAIN TUBECollect just before next dose. Note dosage, timeof dose and collection time on referral.PLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBERefer patient to collection centrePLAIN TUBE OR GEL TUBE2x DRY SWAB - NOSE & THROATLabel swabs with site of collection & test.PLAIN TUBE OR GEL TUBENo Medicare rebate available.YELLOW SCREW-CAPPED SPECIMENCONTAINER - INSECTNo Medicare rebate available.For live insect place in container. For dead insectplace in container with alcohol, methylated spiritsor formalin.PLAIN TUBE OR GEL TUBEFasting collection preferred.PLAIN TUBE OR GEL TUBEFasting collection preferred.PLAIN TUBE OR GEL TUBE47

Pathology testsAlphabetically listedTest NameInterleukin 6International Normalised Ratio (INR)Intrinsic Factor Blocking Antibody (IFB Ab)Iodine - Urine 24 hourIodine - Urine RandomIron StudiesISAC Microarray Allergen TestingISH Testing - TissueJAK2 Gene TestJapanese B EncephalitisJoint Fluid M/C/SKetones - PlasmaKetones - Urine RandomKleihauer (Fetomaternal Haemorrhage)KRAS Gene mutationLactateLactate - CSFCollection RequirementsRefer patient to collection centreNo Medicare rebate available.CITRATE TUBETreat as Urgent. Citrate tube must be filled tothe line at the top of the label (fill line) and mixedthoroughly.PLAIN TUBE OR GEL TUBE24 HOUR URINE CONTAINER (NILPRESERVATIVE)Note starting and finishing times on urinecontainer. Random Urine is the preferredcollection.YELLOW SCREW-CAPPED SPECIMENCONTAINER - MORNING URINE (preferred)No Medicare rebate available.PLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBENo Medicare rebate available.Contact a Histopathologist on (02) 9855 5150 forfurther information regarding this test.4mL EDTA TUBE (separate tube required)PLAIN TUBE OR GEL TUBEYELLOW SCREW-CAPPED SPECIMENCONTAINER - JOINT FLUIDFLUORIDE OXALATE TUBEYELLOW SCREW-CAPPED SPECIMENCONTAINER - RANDOM URINE4mL EDTA TUBEFRESH TISSUE OR PARRAFIN EMBEDDEDTISSUEContact laboratory for information.Refer patient to collection centreSTERILE SCREW-CAPPED SPECIMENCONTAINER - CSF48

Test NameLactate Dehydrogenase (LD)Lactate Dehydrogenase Isoenzymes (LDIsoenzymes)Lactate Pyruvate Ratio - Post ExerciseLactate Pyruvate Ratio - Pre Exercise/RandomLamotrigine (LTG)Lead - BloodLead - Urine 24 hourLead - Urine RandomLegionella AntibodyLegionella Culture - SputumLeptinLeptospira AntibodyLipaseLipoprotein (a)LithiumCollection RequirementsPLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBERefer patient to collection centreRefer patient to collection centreLITHIUM HEPARIN TUBECollect just before next dose. Note dosage, timeof dose and collection time on referral.4mL EDTA TUBEFor occupational exposure collect before shift,after showering & wearing clean clothes. Skinaround site must be cleaned thoroughly to avoidcontamination.24 HOUR URINE CONTAINER (NILPRESERVATIVE)Note starting and finishing times on urinecontainer.YELLOW SCREW-CAPPED SPECIMENCONTAINER - MORNING URINE (preferred)PLAIN TUBE OR GEL TUBEYELLOW SCREW-CAPPED SPECIMENCONTAINER - SPUTUMAn early morning deep cough specimen collectedprior to breakfast is preferred. The mouth shouldbe rinsed thoroughly with water prior to collection.Refer patient to collection centreNo Medicare rebate available.PLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBENo Medicare rebate available.PLAIN TUBE OR GEL TUBECollect just before next dose or 12 hours postdose. Note dosage, time of dose and collectiontime on referral.49

Pathology testsAlphabetically listedTest NameLiver Function Test (LFT)Liver Kidney Microsomal Antibodies (LKM)Lupus InhibitorLuteinising Hormone (LH)Lyme Borreliosis AntibodyLymphocyte Surface Markers (LSM) - BloodLymphocyte Surface Markers (LSM) - BoneMarrowLymphocyte Surface Markers (LSM) - CSFLymphocyte Surface Markers (LSM) - TissueMagnesiumMagnesium - Red CellMagnesium - Urine 24 hourMagnesium - Urine RandomMalarial ParasitesManganese - PlasmaManganese - Urine 24 hourManganese - Urine RandomCollection RequirementsPLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBERefer patient to collection centrePLAIN TUBE OR GEL TUBEIf female, include LMP & any exogenous hormonetherapy on referral.PLAIN TUBE OR GEL TUBENote on the referral if patient has had a ‘tick bite’.4mL EDTA TUBE (separate tube required)BONE MARROW IN LITHIUM HEPARIN TUBE(WITH RPMI MEDIUM)STERILE SCREW-CAPPED SPECIMENCONTAINER - CSFTISSUE IN HANKS SOLUTION OR SALINEPLAIN TUBE OR GEL TUBERefer patient to collection centre24 HOUR URINE CONTAINER (NILPRESERVATIVE)Note starting and finishing times on urinecontainer.YELLOW SCREW-CAPPED SPECIMENCONTAINER - MORNING URINENote time of collection on jar.4mL EDTA TUBETreat as URGENT.Refer patient to collection centre24 HOUR URINE CONTAINER (NILPRESERVATIVE)Note starting and finishing times on urinecontainer.YELLOW SCREW-CAPPED SPECIMENCONTAINER - RANDOM URINE50

Test NameMeasles Antigen - SwabMeasles Antigen - Urine RandomMeasles IgG Antibody (Immunity)Measles IgG/IgM AntibodyMeasles, Mumps & Rubella Immunity ScreenIgGMeningococcal AntibodyMeningococcal PCRMeningococcal PCR - CSFMercury - BloodMercury - Urine 24 hourMercury - Urine RandomMesomarkMetabolic Screen (

Pathology testsAlphabetically listedTest NameMethotrexateMethylene Tetrahydrofolate Reductase(MTHFR) includes both mutations A1298C &C677TMethylmalonic AcidMianserinMicroalbumin - Urine 24 hourMicroalbumin - Urine RandomMicroalbumin - Urine Timed (preferred)Mitochondrial Antibody (AMA)MRSA Screen (Methicillin ResistantStaphylococcus Aureus)Mumps IgG Antibody (Immunity)Mumps IgG/IgM AntibodyMusk AntibodyMycoplasma and Ureaplasma PCR (Genital)Mycoplasma Pneumoniae AntibodyMyelin AntibodyMyoglobin - SerumCollection RequirementsPLAIN TUBECollect as requested. Note dosage, time of doseand collection time on referral.4mL EDTA TUBE (separate tube required)Medicare Criteria:• proven DVT/PE (deep vein thrombosis/pulmonary embolism) in patient or• presence of mutation in first degree relativeRefer patient to collection centrePLAIN TUBECollect just before next dose. Note dosage, timeof dose and collection time on referral.24 HOUR URINE CONTAINER (NILPRESERVATIVE)Note starting and finishing times on urinecontainer.YELLOW SCREW-CAPPED SPECIMENCONTAINER - MORNING URINE (preferred)TIMED OVERNIGHT URINE CONTAINER (NILPRESERVATIVE)Note starting and finishing times on urinecontainer.PLAIN TUBE OR GEL TUBE3x BACTERIAL SWABS (BLUE)Label site of collection on each swab.PLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBERefer patient to collection centreDRY SWAB OR URINEThe preferred collection is 1x DRY swab for eachPCR test. Label swab with site of collection & test.PLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBE52

Test NameMyoglobin - Urine RandomN TelopeptideNarcolepsy Tissue TypingNeuronal AntibodyNeurone Specific Enolase (NSE)Neutrophil Alkaline Phosphatase (NAPScore)NickelNickel - Urine 24 hourNickel - Urine RandomNipple DischargeNipple Fluid M/C/SNormal Protein Catabolic RateNortriptylineOestradiol (E2)OlanzapineOligosaccharides - Urine RandomCollection RequirementsYELLOW SCREW-CAPPED SPECIMENCONTAINER - RANDOM URINEYELLOW SCREW-CAPPED SPECIMENCONTAINER - SECOND VOID MORNING URINE4mL EDTA TUBE (separate tube required)GEL TUBE OR PLAIN TUBERefer patient to collection centreRefer patient to collection centreRefer patient to collection centre24 HOUR URINE CONTAINER (NILPRESERVATIVE)Note starting and finishing times on urinecontainer.YELLOW SCREW-CAPPED SPECIMENCONTAINER - MORNING URINE (preferred)See Smear Cytology - Non GynaeYELLOW SCREW-CAPPED SPECIMENCONTAINER - NIPPLE DISCHARGE24 HOUR URINE CONTAINER (NILPRESERVATIVE)Note starting and finishing times on urinecontainer.PLAIN TUBECollect just before next dose. Note dosage, timeof dose and collection time on referral.PLAIN TUBE OR GEL TUBEIf female, include LMP & any exogenous hormonetherapy on referral.PLAIN TUBECollect just before next dose. Note dosage, timeof dose and collection time on referral.Refer patient to collection centreNo Medicare rebate available.53

Pathology testsAlphabetically listedTest NameOptical Platelet CountOrganochlorine Insecticides - BloodOrganometals - Urine RandomOrganophosphates (Alkyl Phosphates) -Urine RandomOrganophosphates - BloodOsmolalityOsmolality - Urine RandomOsteocalcinOvarian AntibodyOxalate - Urine 24 hourOxalate - Urine RandomPancreatic Islet Cell Antibody (PIC Ab)Pancreatic PolypeptideCollection Requirements4mL EDTA TUBELITHIUM HEPARIN TUBENo Medicare rebate available.Collect at end of shift or following exposureMonday to Thursday only.YELLOW SCREW-CAPPED SPECIMENCONTAINER - RANDOM URINECollect Monday to Thursday only, at end ofshift or following exposure. No Medicare rebateavailable.YELLOW SCREW-CAPPED SPECIMENCONTAINER - RANDOM URINENo Medicare rebate available.LITHIUM HEPARIN TUBECollect Monday to Thursday only.PLAIN TUBE OR GEL TUBEWhen both urine and serum Osmolalityrequested, it is preferred they be collected at thesame time.YELLOW SCREW-CAPPED SPECIMENCONTAINER - RANDOM URINEWhen both urine and serum Osmolalityrequested, it is preferred they be collected at thesame time.Refer patient to collection centreNo Medicare rebate available.PLAIN TUBE OR GEL TUBE24 HOUR URINE CONTAINER (NILPRESERVATIVE)Note starting and finishing times on urinecontainer.YELLOW SCREW-CAPPED SPECIMENCONTAINER - MORNING URINENote time of collection on jar.PLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEFasting & no smoking for 12 hours.54

Test NamePap Smear (PAP)ParacetamolParasite Identification - ScabiesParathyroid Hormone (PTH)Paroxysmal Nocturnal Haemoglobinuria(PNH)Parvovirus IgG Antibody (Immunity)Parvovirus IgG/IgM AntibodyPeriodic Acid Schiff’s Stain (PAS Stain) - NailClippingspH - Gastric FluidpH - Urine 24 hourPharmacogenomic Testing (CYP2C19)Pharmacogenomic Testing (CYP2C9)(Warfarin)Pharmacogenomic Testing (CYP2D6)(Tamoxifen)Pharmacogenomic Testing (DPYD)Collection RequirementsPAP SMEARClearly label slide in pencil with patient nameand date of birth (do not use pen, texta or asticker). Fix immediately after collection using aspray fixative or immerse in 95% alcohol for 20minutes. Allow to dry completely before placing inslide container. DO NOT place slide in the samespecimen bag as a container with Formalin.PLAIN TUBE OR GEL TUBEFor toxicity collect as soon as possible and treatas Urgent.BLACK BOX CONTAINER - SKIN SCRAPINGS6mL EDTA TUBE & PLAIN TUBE OR GEL TUBECollect without tourniquet as PTH requires astasis free calcium.4mL EDTA TUBE (separate tube required)PLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEBLACK BOX CONTAINER - NAIL CLIPPINGSNo Medicare rebate available.YELLOW SCREW-CAPPED SPECIMENCONTAINER - GASTRIC FLUID24 HOUR URINE CONTAINER (NILPRESERVATIVE)Note starting and finishing times on urinecontainer.4mL EDTA TUBE (separate tube required)No Medicare rebate available.4mL EDTA TUBE (separate tube required)No Medicare rebate available.4mL EDTA TUBE (separate tube required)No Medicare rebate available.4mL EDTA TUBE (separate tube required)No Medicare rebate available.55

Pathology testsAlphabetically listedTest NamePharmacogenomics Testing (UGT1A1)PhenobarbitonePhenylalanine - Urine RandomPhenytoinphi (Prostate Health Index)PhosphatePhosphate - Urine 24 hourPhosphate - Urine RandomPhospholipid AntibodyPinworm IdentificationPlatelet Antibody - Non PregnantPlatelet Antibody - Post TransfusionPlatelet Antibody - PregnantPlatelet Count (Plt)Platelet Function Analysis (PFA100)Platelet Serotonin EstimationCollection Requirements4mL EDTA TUBE (separate tube required)No Medicare rebate available.PLAIN TUBE OR GEL TUBECollect just before next dose or at least 6 hourspost dose. Note dosage, time of dose andcollection time on referral.YELLOW SCREW-CAPPED SPECIMENCONTAINER - MORNING URINE (preferred)PLAIN TUBE OR GEL TUBECollect just before next dose or at least 6 hourspost dose. Note dosage, time of dose andcollection time on referral.Refer patient to collection centreNo Medicare rebate available.PLAIN TUBE OR GEL TUBE24 HOUR URINE CONTAINER (NILPRESERVATIVE)Note starting and finishing times on urinecontainer.YELLOW SCREW-CAPPED SPECIMENCONTAINER - MORNING URINENote time of collection on jar.Refer patient to collection centreRefer patient to collection centreRefer patient to collection centreRefer patient to collection centreNo Medicare rebate available.Refer patient to collection centreNo Medicare rebate available.4mL EDTA TUBENote: if clumped platelets also collect an ACDtube.Refer patient to collection centre4mL EDTA TUBE56

Test NamePlatinumPneumococcal AntibodiesPolymyositis Antibody (PM-SCL)Porphyrins - FaecesPorphyrins - Red CellPorphyrins - Urine RandomPotassiumPotassium - CSFPotassium - Gastric FluidPotassium - Urine 24 hourPotassium - Urine RandomPrenatal FISH (Aminotic fluid or CVS)PrimidoneProcalcitoninCollection Requirements4mL EDTA TUBE (separate tube required)PLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBERefer patient to collection centreNote relevant clinical features on referral.4mL EDTA TUBE (separate tube required)Wrap in foil. Note relevant clinical features onreferral.Refer patient to collection centreNote relevant clinical features on referral.PLAIN TUBE OR GEL TUBESTERILE SCREW-CAPPED SPECIMENCONTAINER - CSFYELLOW SCREW-CAPPED SPECIMENCONTAINER - BODY FLUID24 HOUR URINE CONTAINER (NILPRESERVATIVE)Note starting and finishing times on urinecontainer.YELLOW SCREW-CAPPED SPECIMENCONTAINER - MORNING URINENote time of collection on jar.STERILE SCREW-CAPPED SPECIMENCONTAINER - CHORIONIC VILLI SAMPLING(CVS) OR AMNIOTIC FLUIDContact Pre-Natal Testing Department on(02) 9855 5369.No Medicare rebate available.PLAIN TUBE OR GEL TUBECollect just before next dose or at least 6 hourspost dose. Note dosage, time of dose andcollection time on referral.PLAIN TUBE OR GEL TUBENo Medicare rebate available.57

Pathology testsAlphabetically listedTest NameProcollagen Type 3 Intact N-TerminalPropeptide (P3NP)Collection RequirementsPLAIN TUBE OR GEL TUBENo Medicare rebate available.Progensa PCA3 Score Contact Sonic Genetics PCA3 lab (02) 9855 6280for all enquiries.No Medicare rebate available.Progesterone (P2)Progesterone (P2) - Day 21ProlactinProstate Specific Antigen (PSA)Prostatic Specific Antigen Free/Total Ratio(Free PSA)ProteinProtein & Glucose - CSFProtein - Body FluidPLAIN TUBE OR GEL TUBEIf female, include LMP & any exogenous hormonetherapy on referral.PLAIN TUBE OR GEL TUBECollect on day 21 of menstrual cycle.PLAIN TUBE OR GEL TUBEPatient should rest for 15 minutes prior tocollection.PLAIN TUBE OR GEL TUBEFree PSA will only be done if Medicare criteria ismet.Medicare criteria:• Total PSA is above the age-related medianand below or equal to the age-related upperreference limit. (1 episode in a 12 monthperiod) or• If Total PSA is above the age-related upperreference limit , but below 10 ug/L (up to 4episodes for free PSA in a 12 month periodPLAIN TUBE OR GEL TUBEMedicare Criteria:• Total PSA is above the age-related medianand below or equal to the age-related upperreference limit. (1 episode in a 12 monthperiod) or• If Total PSA is above the age-related upperreference limit , but below 10 ug/L (up to 4episodes for free PSA in a 12 month period.PLAIN TUBE OR GEL TUBESTERILE SCREW-CAPPED SPECIMENCONTAINER - CSFSTERILE SCREW-CAPPED SPECIMENCONTAINER - BODY FLUID58

Test NameProtein - Urine 24 hourProtein CProtein Creatinine Ratio - Urine RandomProtein EPG (Electrophoresis)Protein EPG (Electrophoresis) - CSFProtein EPG (Electrophoresis) - Urine 24hourProtein EPG (Electrophoresis) - UrineRandomProtein Immunofixation Electrophoresis(IFE/IEPG)Protein Immunofixation Electrophoresis(IFE/IEPG) - Urine 24 hourProtein Immunofixation Electrophoresis(IFE/IEPG) - Urine RandomProtein SProthrombin Gene Mutation (PGM)Collection Requirements24 HOUR URINE CONTAINER (NILPRESERVATIVE)Note starting and finishing times on urinecontainer.Refer patient to collection centreMedicare Criteria:• History of venous thromboembolism or• First degree relative who has a proven defectYELLOW SCREW-CAPPED SPECIMENCONTAINER - MORNING URINE (preferred)Note time of collection on jar.PLAIN TUBE OR GEL TUBESTERILE SCREW-CAPPED SPECIMENCONTAINER - CSF and PLAIN TUBE OR GELTUBE24 HOUR URINE CONTAINER (NILPRESERVATIVE)Note starting and finishing times on urinecontainer.YELLOW SCREW-CAPPED SPECIMENCONTAINER - MORNING URINEPLAIN TUBE OR GEL TUBE24 HOUR URINE CONTAINER (NILPRESERVATIVE)Note starting and finishing times on urinecontainer.YELLOW SCREW-CAPPED SPECIMENCONTAINER - MORNING URINERefer patient to collection centreMedicare Criteria:• History of venous thromboembolism or• First degree relative who has a proven defect4mL EDTA TUBE (separate tube required)Medicare Criteria:• detection of a mutation associated withvenous clotting59

Pathology testsAlphabetically listedTest NameProthrombin Time (PT)PyruvateQ Fever AntibodyQuantiferon GoldQuinineRabies AntibodyRAST - GeneralRAST - SpecificRed Cell Morphology - Urine MidstreamReducing substances - FaecesReducing substances - Urine RandomRenin & Aldosterone - PlasmaRenin - PlasmaRespiratory Syncytial Virus Antibody (RSV)Respiratory Virus (Atypical) AntibodiesRespiratory Virus (Typical) AntibodiesCollection RequirementsCITRATE TUBECitrate tube must be filled to the line at the top ofthe label (fill line) and mixed thoroughly.Refer patient to collection centrePLAIN TUBE OR GEL TUBERefer patient to collection centreMedicare Criteria:• patient is immunosuppressedPLAIN TUBE OR GEL TUBECollect as requested. Note dosage, time of doseand collection time on referral.PLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEMedicare reimburses for requests up to 5 coreindividual, or 2 core mixes, or 1 core mix and 3individual, or 1 esoteric allergen per episode.PLAIN TUBE OR GEL TUBEMedicare reimburses for requests up to 5 coreindividual, or 2 core mixes, or 1 core mix and 3individual, or 1 esoteric allergen per episode.YELLOW SCREW-CAPPED SPECIMENCONTAINER - MIDSTREAM URINE (Freshspecimen required transport ASAP)BROWN TOP CONTAINER - FAECESYELLOW SCREW-CAPPED SPECIMENCONTAINER - MIDSTREAM URINERefer patient to collection centreRefer patient to collection centrePLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEIncludes Influenza & RSV60

Test NameRespiratory Virus PCR - NasopharyngealAspirateRespiratory Virus PCR - SwabReticulocyte Count (Retics)Retinol Binding Protein (RBP)Reverse Triiodothyronine (RT3)Rheumatoid Factor (RF)Rheumatoid Factor (RF) - Synovial FluidRickettsia AntibodyRoss River Virus IgG/IgM Antibody (RRV)Rubella IgG Antibody (Immunity)Rubella IgG/IgM Antibody (RUB)Salivary Gland AntibodySalmonella AntibodySchistosoma AntibodySchistosoma Parasite - Urine RandomSeleniumCollection RequirementsYELLOW SCREW-CAPPED SPECIMENCONTAINER - NASOPHARYNGEAL ASPIRATEIncludes Influenza A RNA (H1N1/H3N2),Influenza B RNA, Parainfluenza RNA, RSVRNA (Respiratory Syncytial Virus), HumanMetapneumovirus RNA, & Rhinovirus RNA2x DRY SWABS - NOSE & THROATIncludes Influenza A RNA (H1N1/H3N2),Influenza B RNA, Parainfluenza RNA, RSVRNA (Respiratory Syncytial Virus), HumanMetapneumovirus RNA, & Rhinovirus RNA4mL EDTA TUBEPLAIN TUBE OR GEL TUBENo Medicare rebate available.PLAIN TUBE OR GEL TUBENo Medicare rebate available. Note thyroid history& medications.PLAIN TUBE OR GEL TUBEYELLOW SCREW-CAPPED SPECIMENCONTAINER - SYNOVIAL FLUIDPLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEYELLOW SCREW-CAPPED SPECIMENCONTAINER - ENDSTREAM URINEPatient must include last part of urine in container.Refer patient to collection centre61

Pathology testsAlphabetically listedTest NameSelenium - Urine 24 hourSelenium - Urine RandomSemen Analysis (Fertility)Semen Analysis (Post Vasectomy)Semen M/C/SSerotonin - SerumSerotonin - Urine 24 hourSex Hormone Binding Globulin (SHBG)Sexually Transmitted Disease Screen (STD)Silver - Urine 24 hourSirolimusSkeletal Muscle Antibody (SKMA)Skin Auto Antibodies (SICA/BMA)Collection Requirements24 HOUR URINE CONTAINER (NILPRESERVATIVE)Note starting and finishing times on urinecontainer.YELLOW SCREW-CAPPED SPECIMENCONTAINER - MORNING URINE (preferred)Refer patient to collection centreRefer patient to collection centreRefer patient to collection centrePLAIN TUBE OR GEL TUBE24 HOUR URINE CONTAINER (HCLPRESERVATIVE)Note starting and finishing times on urinecontainer. Patient to follow 5HIAA/5HT (Serotonin)dietary advice sheet.PLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBE & YELLOW SCREW-CAPPED SPECIMEN CONTAINER - FIRST VOIDURINE24 HOUR URINE CONTAINER (NILPRESERVATIVE)Note starting and finishing times on urinecontainer.4mL EDTA TUBE (separate tube preferred)Collect just before next dose or as required.Note dosage, time of dose and collection time onreferral.PLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBE62

Test NameSmear Cytology - GynaeSmear Cytology - Non GynaeSmooth Muscle Antibody (SMA)SodiumSodium - CSFSodium - Gastric FluidSodium - Urine 24 hourSodium - Urine RandomSoluble Transferrin ReceptorCollection RequirementsGYNAECOLOGICAL SMEARLabel slides clearly in pencil with patient name,date of birth specimen type and site. Collect usingan endometrial sampling device or endocervicalbrush for vulval smears. Make slides by rollingthe brush over a glass slide. Discard brush. Aseparate slide for each site is required if multiplesites are requested. Fix slides immediately withcytology spray fixative.NON GYNAE SMEARNipple discharge - label slides with patientname, date of birth and type and site of smear.Ask patient to express fluid by massaging thebreast firmly from the base of the breast towardthe nipple. When discharge appears pleasenote on the request form if it is originating froma single duct opening or multiple ducts. Passslide/s lightly over the discharge to collect a directsmear of the material. Quickly fix specimen withspray fixative. Allow to dry before placing in aslide container and forwarding to the laboratory.For other smear types - label slides as describedabove and collect specimen with a cytobrush (lip,mouth and skin lesions), spatula (buccal smear) orDacron swab (anal smear). Fix immediately.PLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBESTERILE SCREW-CAPPED SPECIMENCONTAINER - CSFYELLOW SCREW-CAPPED SPECIMENCONTAINER - BODY FLUID24 HOUR URINE CONTAINER (NILPRESERVATIVE)Note starting and finishing times on urinecontainer.YELLOW SCREW-CAPPED SPECIMENCONTAINER - MORNING URINENote time of collection on jar.PLAIN TUBE OR GEL TUBENo Medicare rebate available.63

Pathology testsAlphabetically listedTest NameSolvent Screen - Urine RandomSpecific Gravity - Urine RandomSperm Antibody (SAB) - SemenSperm Antibody (SAB) - SerumSputum CytologySputum M/C/SStrongyloides AntibodyStrongyloides CultureSwab M/C/S - Ear, Nose, ThroatSwab M/C/S - GenitalSwab M/C/S - WoundSynacthen Stimulation TestSynacthen Stimulation Test with 17OHSyphilis SerologyCollection RequirementsYELLOW SCREW-CAPPED SPECIMENCONTAINER - RANDOM URINECollect Monday to Thursday only, at end ofshift or following exposure. No Medicare rebateavailable.YELLOW SCREW-CAPPED SPECIMENCONTAINER - MIDSTREAM URINERefer patient to collection centrePLAIN TUBE OR GEL TUBEYELLOW SCREW-CAPPED SPECIMENCONTAINER - SPUTUMRoutine collection consists of three separatespecimens preferably collected on 3 consecutivedays. An early morning deep cough specimencollected prior to breakfast is preferred. Themouth should be rinsed thoroughly with waterprior to collection.YELLOW SCREW-CAPPED SPECIMENCONTAINER - SPUTUMPLAIN TUBE OR GEL TUBEBROWN TOP CONTAINER - FAECESBACTERIAL SWAB (BLUE)Label swab with site of collection.BACTERIAL SWAB (BLUE)Label swab with site of collection.BACTERIAL SWAB (BLUE)Label swab with site of collection.Contact local laboratory for booking informationand additional paperwork. (Limited collectionlocations)Partial Medicare Rebate.Contact local laboratory for booking informationand additional paperwork. (Limited collectionlocations)Partial Medicare Rebate.PLAIN TUBE OR GEL TUBE64

Test NameTacrolimusTay-Sachs DiseaseTemazepamTestosteroneTetanus AntibodyThalassaemia Screen (HBEPG)ThalliumThallium - Urine 24 hourThallium - Urine RandomTheophyllineThinPrep PAP TestCollection Requirements4mL EDTA TUBE (separate tube preferred)Collect just before next dose or as required.Note dosage, time of dose and collection time onreferral.Refer patient to collection centreNo Medicare rebate available.PLAIN TUBECollect just before next dose. Note dosage, timeof dose and collection time on referral.PLAIN TUBE OR GEL TUBEPreferred morning collection for male patients.PLAIN TUBE OR GEL TUBE4mL EDTA TUBE & PLAIN TUBE OR GEL TUBEClinical history is required, particularly history ofhaemoglobinopathy and country of origin of thefamily.4mL EDTA TUBE (separate tube required)24 HOUR URINE CONTAINER (NILPRESERVATIVE)Note starting and finishing times on urinecontainer.YELLOW SCREW-CAPPED SPECIMENCONTAINER - MORNING URINE (preferred)PLAIN TUBECollect just before next dose or at least 4 hourspost dose. Note dosage, time of dose andcollection time on referral.THINPREP VIALNo Medicare rebate available.Rinse the Pap smear-taking implement vigorouslyin the ThinPrep vial solution. If both a cervixsampler and an endocervical brush are used,rinse both in the same vial. Discard implement/safter rinsing - do not leave in the vial. ThinPrep vialmay also be used for Chlamydia or GonorrhoeaePCR, Trichomonas vaginalis (TV PCR) and HPVDNA tests.65

Pathology testsAlphabetically listedTest NameThiocyanate - SerumThiocyanate - Urine 24 hourThiocyanate - Urine RandomThiopurine Methyl Transferase EnzymeAssay (TPMT Enzyme Assay)Thiopurine Methyl Transferase Gene Test(TPMT Gene Test)Thrombin TimeThrombophilia StudiesThyroglobulinThyroid AntibodyThyroid Function Test (TFT)Thyroid Stimulating Hormone (TSH)Tin - BloodCollection RequirementsPLAIN TUBE OR GEL TUBE24 HOUR URINE CONTAINER (NILPRESERVATIVE)No Medicare rebate available. Note starting andfinishing times on the container.YELLOW SCREW-CAPPED SPECIMENCONTAINER - RANDOM URINENo Medicare rebate available.Collect at end of shift or following exposureMonday to Thursday only. Note on referral ifpatient is a smoker or non-smoker.4mL EDTA TUBE (separate tube required)4mL EDTA TUBE (separate tube required)CITRATE TUBECitrate tube must be filled to the line at the top ofthe label (fill line) and mixed thoroughly.Refer patient to collection centreMedicare criteria will apply for Thrombophiliatests such as Protein C & S, APC Resistance, AntiThrombin 3, FVL & PGM.PLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEThyroid history must be included on referral for fullThyroid testing.Relevant clinical notes include:• TSH is abnormal or• monitoring thyroid disease or• psychiatric investigation or dementia or• infertility investigation or amenorrhoea or• pituitary dysfunction suspected or• on drugs interfering with thyroid function or• investigating sick euthyroid syndrome inadmitted patientPLAIN TUBE OR GEL TUBE4mL EDTA TUBE (separate tube required)66

Test NameTobramycinTopamaxToxocara AntibodyToxoplasma IgG/IgM Antibody (TOXO)Trace Elements (Zn,Cu,Al,Se) - 24 hour UrineTrace Elements (Zn,Cu,Al,Se) - PlasmaTrichomonas Vaginalis PCRTriglyceridesTrimipramineTroponin TTryptaseTSH Receptor Antibody (TRAB)Tumour Marker TestingUreaUrea - Gastric FluidCollection RequirementsPLAIN TUBECollect just before next dose. Note dosage, timeof dose and collection time on referral.PLAIN TUBECollect 2-4 hours post dose. Note dosage, time ofdose and collection time on referral.PLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBE24 HOUR URINE CONTAINER (NILPRESERVATIVE)Note starting and finishing times on urinecontainer. Note specific metals on referral.Refer patient to collection centreNote specific trace elements on referral.DRY SWAB, FIRST VOID URINE OR THINPREPVIALLabel swab with site of collection & test.PLAIN TUBE OR GEL TUBENote if fasting or random collection.PLAIN TUBECollect just before next dose. Note dosage, timeof dose and collection time on referral.PLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBECollect within 1-4 hours of an attack if possible.PLAIN TUBE OR GEL TUBESee specific tests for specimen requirements.Medicare criteria:Test(s) performed in the monitoring of malignancyor in the detection or monitoring of hepatictumours, gestational trophoblastic disease orgerm cell tumour.PLAIN TUBE OR GEL TUBEYELLOW SCREW-CAPPED SPECIMENCONTAINER - BODY FLUID67

Pathology testsAlphabetically listedTest NameUrea - Urine 24 hourUrea - Urine RandomUrea Breath Test C14 (UBT)Uric AcidUric Acid - Body FluidUric Acid - Urine 24 hourUric Acid - Urine RandomUrine CytologyUrine M/C/SValproateCollection Requirements24 HOUR URINE CONTAINER (NILPRESERVATIVE)Note starting and finishing times on urinecontainer.YELLOW SCREW-CAPPED SPECIMENCONTAINER - MORNING URINENote time of collection on jar.Refer patient to collection centrePLAIN TUBE OR GEL TUBEYELLOW SCREW-CAPPED SPECIMENCONTAINER - BODY FLUID24 HOUR URINE CONTAINER (NILPRESERVATIVE)Note starting and finishing times on urinecontainer.YELLOW SCREW-CAPPED SPECIMENCONTAINER - MORNING URINENote time of collection on jar.500mL STERILE CONTAINER (URINE CYTOLOGYWHITE TOP)Routine urine cytology consists of three separatespecimens preferably collected on 3 consecutivedays. Do not collect the first morning urine as thiswill contain degenerate cells. Collect a specimenafter the patient has been hydrated and ambulant.(Ambulation encourages exfoliation of cells).Collect the entire bladder volume into 500mLwhite top container. If using a smaller urine jar,collect the midstream part of the void.YELLOW SCREW-CAPPED SPECIMENCONTAINER - MIDSTREAM URINEPLAIN TUBE OR GEL TUBECollect just before next dose or at least 6 hourspost dose. Note dosage, time of dose andcollection time on referral.68

Test NameVancomycinVancomycin Resistant Enterococci (VRE)Varicella Zoster IgG Antibody (VCZ)(Immunity)Varicella Zoster IgG/IgM Antibody (VCZ)Varicella Zoster PCR (VCZ PCR)Vasoactive Intestinal Peptide (VIP)Vault SmearVerifi Fetal DNA Testing (NIPT)VigabatrinViral CultureViscosity - Serum/PlasmaVitamin AVitamin B1Vitamin B12Collection RequirementsPLAIN TUBE OR GEL TUBECollect trough level just before next dose. Peaklevel is collected 1/2 to 1 hours post dose. Notedosage, time of dose and collection time onreferral.BROWN TOP CONTAINER - FAECES ORBACTERIAL SWAB (BLUE)PLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBEDRY SWAB OR BACTERIAL SWAB (BLUE)The preferred collection is 1x DRY swab for eachPCR test except when Herpes requested withVaricella. Label swab with site of collection & test.Refer patient to collection centreVAULT SMEARClearly label slide in pencil with patient nameand date of birth (do not use pen, texta or asticker). Fix immediately after collection using aspray fixative or immerse in 95% alcohol for 20minutes. Allow to dry completely before placing inslide container. DO NOT place slide in the samespecimen bag as a container with formalin.Contact Sonic Genetics on 1800 010 447 or(02) 9855 5369 for all test enquiries.No Medicare rebate availablePLAIN TUBECollect just before next dose. Note dosage, timeof dose and collection time on referral.PCR has replaced Viral Culture in most situations,please consult a microbiologist on (02) 9855 5312for further information.Refer patient to collection centreRefer patient to collection centreRefer patient to collection centrePLAIN TUBE OR GEL TUBE69

Pathology testsAlphabetically listedTest NameVitamin B12 & Red Cell Folate (RCF)Vitamin B2Vitamin B3 - Urine 24 hourVitamin B6Vitamin CVitamin D (25OH Vit D)Vitamin D MetaboliteVitamin EVon Willebrand Studies (VWS)Washings CytologyWater TestingWeedicide/Herbicides - Urine RandomYersinia enterocolitica SerologyZAP-70ZincZinc - PlasmaZinc - Red CellZinc - Urine 24 hourZinc - Urine RandomCollection Requirements4mL EDTA TUBE & PLAIN TUBE OR GEL TUBERefer patient to collection centre24 HOUR URINE CONTAINER (HCLPRESERVATIVE)Note starting and finishing times on urinecontainer.Refer patient to collection centreRefer patient to collection centrePLAIN TUBE OR GEL TUBEPLAIN TUBE OR GEL TUBERefer patient to collection centreRefer patient to collection centreSee Fluid CytologyContact Sonic Food & Water Testing - Penrith forenquiries on 1800 048 993 or (02) 4734 6582.No Medicare rebate available.YELLOW SCREW-CAPPED SPECIMENCONTAINER - RANDOM URINESpecify weedicide or herbicide exposure. CollectMonday to Thursday only, at end of shift orfollowing exposure. No Medicare rebate available.PLAIN TUBE OR GEL TUBERefer patient to collection centreRefer patient to collection centreRefer patient to collection centreRefer patient to collection centre24 HOUR URINE CONTAINER (NILPRESERVATIVE)Note starting and finishing times on urinecontainer.YELLOW SCREW-CAPPED SPECIMENCONTAINER - MORNING URINE (preferred)70

Test NameZinc ProtoporphyrinZoonosis ScreenCollection Requirements4mL EDTA TUBEPLAIN TUBE OR GEL TUBE71

Specimen storage guideSpecimen typeAspirates/fluidsBlood CulturesBlood SpecimensFaeces – CultureFaeces – Reducing substances (if requested on its own)Pap smearsSputumSwabsUrinePreferred storageRoom TemperatureRoom TemperatureRoom TemperatureRoom TemperatureFreezeRoom TemperatureRoom TemperatureRoom TemperatureRoom Temperature72

Patient InstructionsPlease refer to our website; www.sonichealthcare.com.au/dhm for detailed information on patientinstructions, or to print out a copy for your patient.Fasting1. Fasting is generally overnight and the blood collected or test performed for the following morning.2. Fasting is usually for a minimum of 8 hours although preferably for 12 hours unless otherwise statedby your Doctor.3. All food and beverages should be withheld during the fasting period with the exception of water.4. Medication should only be stopped on the instructions of your Doctor.Post blood collection1. Rest your arm.2. Do not lift anything heavy.3. Do not wear tight or restrictive clothing above or around puncture site.4. Avoid strenuous activities or exercise.5. Avoid carrying heavy objects or parcels.Note: A few people do bruise easily after blood tests. This is unavoidable. By following the aboveinstructions carefully you will minimise any bruising that may occur. If, however, you experience anyswelling or extensive bruising, please seek medical advice.73

Patient InstructionsDiet sheet glucose tolerance testPlease phone the local collection centre (found at the back of request form) to make anappointment for this test.The 3 day diet is desirable but not essential. For any patient whose doctor indicates that the diet is notrequired, please follow the doctor’s instructions.Pre test preparationA. To make sure that this test gives reliable results, it is important that you eat generous amountsof carbohydrate on each of the three days before the test. The carbohydrate foods that arerecommended are bread, cereal, potatoes, vegetables and fruit. You should have your usualamounts of milk, meat, fish, eggs, cheese, butter and margarine and, as well, eat the followingfoods daily during each of the three days before the test in at least these amounts.• 3 slices of bread or toast• One serving of breakfast cereal or porridge, spaghetti• One medium potato or one serving of rice• 3 servings of vegetables• 3 servings of fruit (fresh, cooked, canned or juice)• For morning tea and afternoon tea have 2 biscuits or 1 scone or an extra slice of bread or toast.B. Do not eat after 9pm on the evening before the test. Only water and usual medication should betaken during the 12 hours before the test.C. No smoking on the day of the test.Important Note: If you should become unwell whilst on the three day high carbohydrate diet ornotice that you are loosing weight, feeling drowsy, thirsty or are passing large amounts of urine pleasecontact your referring doctor immediatelyDuring the test• You will be required to remain in the collection centre for the duration of the test (between two andfive hours depending on the test ordered by your doctor). This is because factors such as exercise,smoking and caffeine consumption can affect the results of the OGTT. Permission to leave thecollection centre during the test will only be given following approval by your doctor.• On arrival at our collection centre you will be required to pass a fasting urine sample beforecommencement of the test and a blood sample will be taken from you by one of our pathologycollection staff. You will then be asked to drink a sweet glucose drink and blood will be collected atappropriate intervals.• We suggest that you bring a book to read.• If you require further information please ring the collection centre at which you are having your test.74

Privacy & PathologyPrivacy & PathologyLegislation has been passed which imposes privacy obligations on the private sector, including thehealth sector. The Privacy Amendment (Private Sector) Act 2000 came into effect on 21 December2001 and at Douglass Hanly Moir Pathology we have put processes in place to ensure that we complywith all of its obligations. Such processes will be subject to continuing review.In summary, the Act sets a minimum standard for the way in which we collect, store, use and disclosepersonal and sensitive health information. The requirements imposed are contained in a set ofprinciples called the “National Privacy Principles”.The information that the Act covers is potentially very wide. We have developed a privacy policy thatdescribes how we manage the following issues:• What personal and sensitive information do we collect• How do we collect such information• What do we use the information for• What reasons are there for disclosing the information• How do we store the information• How do we allow access by our patients and referrers to the information that we holdPatient's contact information may be used for matters relating to billing, particularly with regard to thedelivery of invoices, reminders and expediting of payments. This could, for example, include the use ofSMS reminders and the referral of unpaid invoices to a third party debt collection agency.A brochure detailing our Privacy Policy is readily available to patients at our collection centres and isalso available on our website, www.dhm.com.au/privacy.If you require any further information, please contact ourPrivacy Officer/Quality Department on 98 555 222.75

Swab guideUse Swab type InstructionsBacterial swab – microscopy and cultureCulture & SensitivityMost sites and lesionsBlue Top SwabProduct #3338Please refer to instructionson packagingTo collect nasopharyngeal swab for culture and sensitivity please use orange top swab (Thin Shaft)– available upon request – Stores Order #1299 and place in the medium provided with blue swab Stores Order #3338PCR swab (flocked swab, no transport medium)Herpes simplex and/orVaricella zosterChlamydia trachomatis and/orN.gonorrhoeaeBordetella pertussisRespiratory virusesTrichomonas vaginalis1 PCR swab preferred for each testRed Top Swab– supplied by default _Stores Order #5290Orange Top Swab (Thin Shaft)– available upon request –Stores Order #1299Swab from lesionCervical/urethal/eye swabPosterior pharynxor nasopharynx swabThroat and nose swabsVaginal/urethal swabSwab for other viral tests – the majority of viruses are now detected by PCR as aboveAntigen Detectione.g. measlesViral CulturePlease contact microbiologist02 98 555 312Green Top SwabProduct #3734Please refer to instructions onpackaging76

Tube GuideIncluding recommended order of drawHemogardClosure & TubeContentCatalogueNumber &VolumeDeterminationsInstructionsBlood CultureBottles442192 Aerobic(10mL):442265 Anaerobic(10mL):442194 (pink) PedsPlus (1-3mL):MicrobiologyTo exclude the possibility of septicaemia1 x set of blood culture bottles: Both Aerobic andAnaerobicUsing a butterfly scalp vein needle only add 8-10mLs blood to each bottle (do not overfill) Collectaerobic bottle first and mix gently by inverting thebottles 5 timesStorage and Transportation: Room TemperaturePaediatric bottles are available on request andmust be filled with 1-3 mLs of bloodCitrate363095 (2.7mL)CoagulationPR, APTT, INR, Coagulation Studies, D-Dimer(Note : Coagulation Studies require 2 citrate tubesand a 4mL EDTA tube)Fill to at least Minimum Fill and not overMaximum Fill Line marked on the tubeMIX WELL BY GENTLY INVERTING 6 TIMES368985 (6mL)Platelet estimation on patients with known historyof EDTA induced platelet clumpingMIX WELL BY GENTLY INVERTING 6 TIMESACDPlainGel367895 (10mL)367958 (8.5mL)General BiochemistryCardiac Markers, U&E, LFT, Lipids, HDL, Amylase,Lipase, Magnesium, Fe Studies, TherapeuticDrugs, CRP, Vitamin D, Vitamin B12, GlucoseGeneral SerologyGeneral Serology, Hormones, Hepatitis Serology,RAST, HIVGeneral Biochemistry & SerologyAs above, however,NO THERAPEUTIC DRUG LEVELS on this tubeNumber of tubes required:• Up to 6 test groups – 1 tubee.g. LFT, Hepatitis, Amylase, TFT,Hormones, Cardiac Enzymes• More than 6 test groups – 2 tubesLithium HeparinEDTA367885 (6mL)367839 (4mL)BiochemistryCholinesterase (Red Cell or Plasma), ChromosomeAnalysis, OrganophosphatesHaematologyFBC, Hb, WCC, Diff, Platelets, ESR, MalarialParasites, ReticsBiochemistryGlycated Hb (HbA1c), Red Cell Folate, Lead, FEP,G6PD, Porphryins (Red Cell)Molecular Genetics*(EDTA acceptable, however separate red-rimmedEDTA preferred)MIX WELL BY GENTLY INVERTING 6 TIMESMIX WELL BY GENTLY INVERTING 6 TIMESEDTA367941 (6mL)Blood BankCross Match, Group & Hold, Antibody Screen,Blood Group (Note: 6mL EDTA and 4mL EDTAtube required for all crossmatches and Group& Hold)General SerologyPTH (additional plain or gel tube required as well)MIX WELL BY GENTLY INVERTING 6 TIMESNote: Full patient identification required forCrossmatching and Group & Hold, Blood Group& Ab ScreenDoctor/Collector must initial tube to verifypatient detailsTransfusion form required with any Cross Match orGroup & Hold requestRed Rimmed EDTA454003 (4mL)Flow CytometryLymphocyte Surface Markers (additional 4mLEDTA tube required for WCC), CD59, ParoxysmalNocturnal Haemoglobinuria*Molecular Genetics (separate tube required)Haemochromatosis, Factor V Leiden, MTHFR,PGM, HLA B27, Fragile X, Coeliac Tissue TypingMIX WELL BY GENTLY INVERTING 6 TIMES367935 (5mL)BiochemistryGlucose, Blood Alcohol (Medical)MIX WELL BY GENTLY INVERTING 6 TIMESFluoride Oxalate

DOUGLASS HANLY MOIR PATHOLOGY • ABN 80 003 332 858A subsidiary of SONIC HEALTHCARE14 GIFFNOCK AVENUE • MACQUARIE PARK • NSW 2113 • AUSTRALIATEL (02) 98 555 222 • FAX (02) 9878 5077MAIL ADDRESS • LOCKED BAG 145 • NORTH RYDE • NSW 1670 • AUSTRALIABARRATT & SMITH PATHOLOGY • ABN 80 003 332 858A trading name of DOUGLASS HANLY MOIR PATHOLOGYA subsidiary of SONIC HEALTHCARE31 LAWSON STREET • PENRITH • NSW 2750 • AUSTRALIATEL (02) 4734 6500 • FAX (02) 4732 2503MAIL ADDRESS • PO BOX 443 • PENRITH • NSW 2751 • AUSTRALIACobrand-PCG-V1-2013-1023

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