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BD Biosciences - Discovery Labware BD Falcon In Vitro Fertilization Plasticware • Pre‑tested and certified plasticware for in vitro fertilization (IVF) • Ce marked to the Medical Device Directive 93/42/eC Features • Non‑embryotoxic • Non‑pyrogenic • Non‑cytotoxic • Tissue culture‑treated for a consistent hydrophilic surface • Sterilized by gamma irradiation • Packaged in peel‑open, medical‑style packaging • Multi‑unit bags have reseal tabs Description Qty./Pack Qty./case cat. no. 60 mm Diameter Dish 20 500 353652 well area: 21.29 cm 2 well Volume: 23.0 ml center-well, 20 500 353653 60 mm Diameter Dish well area: 2.89 cm 2 well Volume: 2.5 ml total Volume: 20 ml 4-well Plate 1 100 353654 well area: 1.39 cm 2 well Volume: 1.8 ml Note: The lids of the BD Falcon IVF Products are not certified non-embryotoxic. BD Falcon cell cultureware Pretested BD Falcon IVF products BD Falcon In Vitro Fertilization (IVF) products are the first plasticware available that is certified sterile, non‑pyrogenic and non‑ embryotoxic. These pre‑tested products save you time and expense in complying with the College of American Pathologists (CAP) and American Fertility Society (AFS) recommended standards for IVF labs. Mouse embryo testing Products are manufactured in compliance with cGMP standards. each lot of BD Falcon IVF product is tested for embryotoxicity using the 1‑cell mouse embryo assay. Mouse embryos are isolated at the 1‑cell stage from B6C3F1 females following super ovulation with gonadotropin and mating with B6D2F1 males. BD Falcon IVF products are tested using both a direct and indirect test method. In the direct assay, the embryos are cultured directly in the IVF labware. For the indirect assay, culture media is incubated in the IVF labware for 24 hours at 37ºC. The incubated media is then transferred to a culture plate and the 1‑cell mouse embryo assay is performed. Products are deemed non‑embryotoxic if they support the growth of more than 75 percent of the embryos to the expanded and/or hatched blastocyst stage. To improve manipulation of ova and embryos, BD Biosciences designed, in conjunction with embryologists, an innovative 4‑well plate. A unique lid reduces the risk of contamination and minimizes evaporation by providing access to two wells at a time, while two remain covered. The wells are numbered and a large writing patch allows clear sample identification. Plates are packaged in individual peel‑open trays for sterile presentation. BD Falcon IVF dishes and 4‑well plates are manufactured from virgin crystalline polystyrene tested for USP Class VI cytotoxicity. They have flat, optically clear surfaces for optimum manipulation and observation of ova and embryos. Lids were designed for aseptic manipulation and consistent venting to maintain humidification. Individual certificates, containing actual test results, are available for each lot by calling your local BD office after you receive your order. 4 IVF bdbiosciences.com | 49
4 BD Primaria B D F a l c o n c e l l c u lt u r e w a r e BD Biosciences - Discovery Labware BD Primaria cultureware • Unique nitrogen‑containing tissue culture surface chemistry • Improves attachment, spreading, and growth for many primary cells or cell lines unique surface chemistry for enhanced cell culture Consistent cell culture conditions are required for reproducible research results. In the manufacture of all cell cultureware surfaces, hydrophobic polystyrene is permanently rendered hydrophilic to support cell attachment and spreading 1,2,3 . The consistency of this surface depends on the treatment method used. Many manufacturers have long used atmospheric plasma treatments (i.e., corona) to create hydrophilic surfaces. In corona treatment, a high‑voltage discharge creates a reactive gas plasma above the growth surface of the vessel. In this process, the highly interactive gasplasma mixture is created from ambient air. The consistency of the treatment surface can therefore be compromised by day‑to‑day environmental changes. At BD Biosciences, molded polystyrene vessels are placed in a chamber where a partial vacuum is created. A vacuum‑gas plasma treatment is fed into the chamber to create a number of negatively charged functional groups on the surface of the polystyrene vessels. The enclosed, highly controlled environment prevents contamination from the ambient air, ensuring a pure and consistent treatment surface. A major research investment by BD Biosciences resulted in the development of this unique vacuum‑gas plasma process used to produce both BD Primaria and traditional tissue culture (TC) surfaces on BD Falcon Dishes, Plates, Flasks and Roller Bottles. The gases used to manufacture BD Primaria contain both oxygen and ammonia, resulting in the incorporation in the surface of a variety of nitrogen‑containing functional groups in addition to the negatively charged oxygen‑containing groups found on traditional TC surfaces. 50 | bdbiosciences.com When chick embryo spinal cord neurons are cultured on BD Primaria (left), growth is enhanced and extensive neurite development occurs. In this experiment, cells clumped and detached from traditional TC plates (above) after 20 days in culture, but remained viable and differentiated on BD Primaria. The incorporation of nitrogen‑containing cations has been correlated to attachment and spreading of primary endothelial cells in a clonal cell‑growth assay 4 . The complex surface on BD Primaria Cultureware is homogeneous and stable and has been in use by researchers for over a decade to improve attachment and differentiation of a variety of cell types. For example, cell biologists have used BD Primaria for cultivating hepatocytes 5,6 , neuronal cells 7 , and other endothelial cells 8 . The surface chemistry of BD Primaria products is confirmed by electron Scanning for Chemical Analysis (eSCA). traditional tissue-culture Surface chemistry BD Primaria Surface chemistry Note: At pH 7, carboxy groups may be slightly dissociated and assume a negative (anionic) charge. Amine groups may protonate and assume a positive charge (cationic). the following is a partial list of cell types cultured on BD Primaria cellware • Hepatocytes 5,6,9,18 • Transfected COS‑7 10 • Transfected HeK‑293 12 • CHO 13 • Primary SMC 14 and Skeletal Muscle Cells 16 • Primary Cardiac Myocytes 15 • Osteoblasts 17 • Neuronal Cells 7 • endothelial Cells 8
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204 Bioimaging SyStemS BD Bioscienc
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9 Pathway Bioimager Bioimaging SySt
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9 aCtone gPCR Screening assay Bioim
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10 Alphabetical Index AlphAbetIcAl
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10 numerical Index nUMeRIcAl Index
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BD Biosciences Worldwide Discovery
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