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BD Biosciences - Discovery Labware
BD cell Mab Medium
• Medium formulated to provide high‑yield monoclonal antibody
(MAb)
Three formulations of BD Cell MAb medium have been developed
to maximize antibody production in highly aerated culture systems.
The CeLLine flask, with aeration occurring through a silicon
membrane in the flask base, is a compact and easy‑to‑use device
that will help optimize antibody production in BD Cell MAb media.
BD Cell media will support a wide variety of myeloma fusion partners
and hydromas, including Sp2/0, NS‑1, P3X63Ag9, and FOX‑NY. In
addition, BD Cell Media have been used successfully for secreted
protein production using CHO cell lines. BD Cell MAb media have
been found to produce as much as ten times more antibody when
compared with conventional media. Due to the unique formulation
of these media, cells can retain viability for longer periods of time
and require much less handling than conventional media.
BD Cell MAb media have been found to work well in cell fusions,
thus eliminating the adaptation process for new clones. Normal
fusion procedures can be followed with the substitution of BD Cell
media.
The three formulations of BD Cell MAb media are offered to suit
either research or production requirements. Serum‑Free and Animal
Component‑Free formulations can be used in production situations
where the variability due to serum or the presence of serum proteins
are undesirable.
BD Falcon cell cultureware
BD cell Basal Mab Medium is a basal medium that requires
serum supplementation.* The serum concentration in the cultivation
compartment should be roughly double your standard serum
concentration. This medium contains L‑glutamine and phenol red.
It does not contain animal components, pluronic acid, or other
surfactants.
the BD cell Serum-Free Mab Medium is a complete medium
that does not require serum supplementation. This medium contains
L‑glutamine and phenol red and has a total protein concentration of
1.1 mg/ml. The majority of the protein is bovine serum albumin. It
does not contain pluronic acid or other surfactants.
the BD cell animal-Free Medium is a complete medium that
does not require serum supplementation. This medium contains
no animal components. BD Cell Animal‑Free Medium contains
L‑glutamine and is supplemented with 0.3% Select Soytone. It
does not contain phenol red, pluronic acid, other surfactants, or
attachment factors.
* The serum supplemented media should only be placed in the cell cultivation chamber of
a CELLine flask.
Description Qty. cat. no.
BD Cell MAb Basal Medium 1000 ml 220511
BD Cell Serum-Free MAb Medium 1000 ml 220509
BD Cell Animal-Free Medium 1000 ml 220513
Yield analysis of BD Cell using
Protein-G purification
4
Mab Medium
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