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BD Biosciences - Discovery Labware BD Falcon BD Falcon cell culture Flasks cell culture Flasks • Sterilized by gamma irradiation • Non‑pyrogenic • Volumetric graduations and writing patch • Vented caps incorporate a 0.2 µm hydrophobic membrane Description cap Style Qty./case cat. no. 12.5 cm2 canted neck total Volume: 25 ml Qty.: 10/bag Standard TC* Plug-seal 100 353018 Standard TC 25 cm Vented 100 353107 2 canted neck total Volume: 50 ml Qty.: 20/bag Standard TC Plug-seal 200 353014 Standard TC Vented 100 353108 BD Primaria TC Plug-seal 200 353813 BD Primaria TC 25 cm Vented 100 353808 2 canted neck total Volume: 70 ml Qty.: 20/bag Standard TC Plug-seal 200 353082 Standard TC Vented 100 353109 Standard TC Phenolic 200 353081 Non-treated 75 cm Plug-seal 200 353009 2 Straight neck total Volume: 250 ml Qty.: 5/bag Standard TC Plug-seal 100 353024 Standard TC Vented 100 353110 Standard TC Phenolic 100 353023 BD Primaria TC Plug-seal 100 353824 BD Primaria TC Vented 100 353810 *TC = Tissue Culture TIPS • Use BD Falcon non-treated cell culture flasks for suspension cultures . • For enhanced cell performance, BD BioCoat Flasks are available with pre-applied matrix proteins . (See Chapter 6) BD Falcon cell cultureware • Phenolic caps contain non‑toxic liners • Manufactured under a registered ISO 9001 Quality System • Growth area and volume are nominal Description 75 cm cap Style Qty./case cat. no. 2 canted neck total Volume: 250 ml Qty.: 5/bag Standard TC Plug-seal 60 353135 Standard TC Vented 60 353136 Standard TC Phenolic 60 353134 Non-treated Plug-seal 60 353133 150 cm2 canted neck total Volume: 600 ml Qty.: 5/bag Standard TC Plug-seal 40 355000 Standard TC Vented 40 355001 175 cm2 Straight neck total Volume: 750 ml Qty.: 5/bag Standard TC Plug-seal 40 353028 Standard TC Vented 40 353112 Standard TC Phenolic 40 353045 Bar-coded 175 cm2 Straight neck - compatible with robotic cultivation systems, e.g. the automation Partnership’s Select total Volume: 750 ml Qty.: 5/bag Standard TC 225 cm Vented 40 353118 2 canted neck total Volume: 800 ml Qty.: 5/bag Standard TC Plug-seal 30 353139 Standard TC Vented 30 353138 300 cm2 Straight neck total Volume: 1900 ml Qty.: 1/bag Standard TC Plug-seal 12 353099 Standard TC Vented 12 353113 4 Flasks bdbiosciences.com | 37
4 Flasks B D F a l c o n c e l l c u lt u r e w a r e BD Biosciences - Discovery Labware BD Falcon Flask caps • A variety of caps to suit your cell culture requirements Plug-Seal caps BD Falcon polyethylene plug‑seal caps provide a liquid‑tight seal when closed and an open‑incubation position for reliable gas exchange when partially opened. A reference bar on the outside of the cap is aligned with the “BD Falcon” on the flask for precise open‑incubation conditions. convenient Vented caps ensure consistent Gas exchange and Minimize contamination Polyethylene caps with an integral, hydrophobic 0.2 µm microporous membrane filter vent allow consistent gas exchange when the caps are in the closed position (Graph 1). Gases required for cell growth and metabolism pass freely through the vent while microorganisms cannot. BD Falcon vented caps will not wet out. Vented caps minimize contamination associated with standard open incubation. The vented caps prevent media that can become trapped in a partially opened cap from blocking gas exchange. The caps will not fall off in the incubator because of vibration. Phenolic caps The rigid cap material allows the cap to be easily spun onto the neck during repetitive manipulations. The special non‑cytotoxic liner in BD Falcon phenolic caps makes a tight seal with the top rim of the flask neck to provide a reliable liquid or gas‑tight seal. 38 | bdbiosciences.com TIPS pH 8.0 7.8 7.6 7.4 7.2 7.0 6.8 6.6 0 Plug-seal, vented, and phenolic caps. Loose Plug-Seal Cap Closed Vented Cap 15 30 45 60 Time (minutes) 90 120 150 180 Graph 1 pH equilibration using vented caps after flasks are placed in an incubator (175 cm 2 Flasks, 5% CO 2 incubator) Vented caps for BD Falcon Flasks Description Qty./case cat. no. For use on 25 cm 2 Flasks 100 354637 For use on 75 cm 2 Flasks 100 354638 For use on 175 cm 2 Flasks 50 354639 Corrugated cardboard boxes are a source of particulates and associated microbial contamination and should not be kept in the tissue culture area . BD Biosciences has eliminated corrugated trays from most cases to reduce package waste . You can further reduce contamination due to corrugate by wiping the outside of any package or bag with alcohol or an appropriate disinfectant before putting it under the hood .
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204 Bioimaging SyStemS BD Bioscienc
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9 Pathway Bioimager Bioimaging SySt
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9 aCtone gPCR Screening assay Bioim
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10 Alphabetical Index AlphAbetIcAl
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10 numerical Index nUMeRIcAl Index
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BD Biosciences Worldwide Discovery
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