BDâ„¢ P100 Blood Collection Kit

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Minimize Preanalytical Variability with BD P100 Blood Collection Kit Human plasma contains a wide variety of proteins that hold potential as biomarkers for proteomics-based diagnostic and prognostic assays. * These proteins are subject to proteolytic degradation and modification during and after blood collection. The use of protease inhibitors – mixed immediately with blood – minimizes preanalytical variability associated with plasma sample preparation and handling. BD P100 v1.1 Blood Collection Kit enables greater recovery and preservation of plasma proteins by immediately mixing with proprietary novel on-board stabilizers, specifically formulated for human plasma. Preserve Valuable Plasma Proteins † Patented * BD P100 is For Research Use Only – Not For Use in Diagnostics Procedures Highlights: • On-board stabilizers provide point-of-collection protection of plasma proteins • Enhances long-term preservation by stabilizing analytes during blood collection • Innovative mechanical separator † minimizes cellular contamination • Eliminates pre-mixing and transfer tubes • Sterile and ready to use BD P100 Blood Collection Tube Specifications: Total Blood Draw Volume: ~8.5 mL Plasma Yield: ~3.5 mL Anticoagulant (Spray Dried): K 2 EDTA Additive (Lyophilized): Proprietary Protein Stabilizers Storage Prior to Use: 2°C to 8°C
BD P100 v1.1 Immediately Stabilizes Plasma Proteins BD P100 v1.1 versus EDTA Plasma “Time 0” Gel Plot Summary of Eight Subjects (m/z 1000-2400) BD P100 v1.1 versus EDTA Plasma “Time 0” Zoom Plot (m/z 1700-2050) Figure 1. Preservation of plasma proteins with BD P100 v1.1 Blood Collection Kit. Blood was drawn directly from eight subjects into P100 and EDTA evacuated tubes. Plasma was processed within 10 minutes of blood collection by centrifugation at 2,500 x g for 15 minutes at room temperature. After blood collection, plasma was transferred into microtubes and prepared for mass spectrometry analysis or stored at –80ºC for future analysis. Peptides were isolated from the plasma samples and analyzed by MALDI-TOF MS. The mass spectra were collected in the mass range from 800 to 3200 m/z. Areas within the red rectangles demonstrate enhanced peptide stabilization in the P100 samples versus EDTA plasma samples. A greater degree of proteolytic degradation is also more apparent in the EDTA plasma samples, even though samples were processed immediately upon blood collection (“Time 0”), as shown in the yellow rectangle areas. Figure 2. Enhanced stability of peptides observed in plasma samples collected with BD P100 v1.1 Blood Collection Kit. Regions with red arrows on this MALDI-TOF MS zoom plot (m/z 1700-2050) of two subjects demonstrates the ability of P100 v1.1 to stabilize plasma peptides more effectively in the presence of protease inhibitors. These peaks are absent in the same regions on the EDTA zoom plots for both subjects. The immediate mixing of blood with the onboard protease inhibitors in BD P100 tubes enhances the recovery of plasma proteins for proteomics studies.
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BDâ„¢ P100 Blood Collection Kit

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