BDâ„¢ P700 Blood Collection System for Plasma GLP-1 Preservation
BDâ„¢ P700 Blood Collection System for Plasma GLP-1 Preservation
BDâ„¢ P700 Blood Collection System for Plasma GLP-1 Preservation
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BD <strong>P700</strong><br />
<strong>Blood</strong> <strong>Collection</strong> <strong>System</strong> <strong>for</strong><br />
<strong>Plasma</strong> <strong>GLP</strong>-1 <strong>Preservation</strong><br />
BD <strong>P700</strong> is <strong>for</strong> Research Use Only — Not <strong>for</strong> Use in Diagnostic Procedures<br />
Preserve<br />
www.bd.com/proteomics
BD <strong>P700</strong> <strong>Blood</strong> <strong>Collection</strong> <strong>System</strong><br />
<strong>for</strong> <strong>Plasma</strong> <strong>GLP</strong>-1 <strong>Preservation</strong><br />
Studies show that BD <strong>P700</strong> enables<br />
significant increase in <strong>GLP</strong>-1 recovery (Table 1).<br />
BD <strong>P700</strong> is a new protein preservation blood<br />
collection tube that contains a proprietary Dipeptidyl<br />
Peptidase IV (DPP-IV) protease inhibitor. The tube<br />
is especially suited as the blood collection tube<br />
of choice <strong>for</strong> assays that require quantitation and<br />
measurement of the preproglucagon-derived glucose<br />
regulatory peptide, glucagon-like peptide 1 (<strong>GLP</strong>-1).<br />
Tube Size 13 x 75 mm plastic tube<br />
Draw Volume 3 mL<br />
Closure translucent lavender BD Hemogard shield<br />
Separator none<br />
Sterilization Sterility Assurance Level (SAL) 10 -6<br />
Anticoagulant 5.4 mg K 2 EDTA (spray coated)<br />
Additive<br />
BD <strong>P700</strong> <strong>Blood</strong> <strong>Collection</strong> Tube Specifications<br />
Storage 2 – 8° C<br />
proprietary Dipeptidyl Peptidase IV (DPP-IV)<br />
inhibitor (lyophilized)<br />
Expire 1 year from date of manufacture
Table 1. Comparison of plasma <strong>GLP</strong>-1 levels obtained from whole blood samples<br />
collected into BD <strong>P700</strong> and K 2 EDTA Tubes. <strong>Blood</strong> was drawn directly from<br />
12 subjects into BD <strong>P700</strong> and K 2 EDTA evacuated tubes at room temperature.<br />
<strong>GLP</strong>-1 was measured using a <strong>GLP</strong>-1 (7-36)-Amide competitive enzyme immunoassay.<br />
BD <br />
<strong>P700</strong><br />
<strong>GLP</strong>-1<br />
(pM/mL)<br />
BD <strong>P700</strong> Enhances <strong>Preservation</strong><br />
of <strong>GLP</strong>-1 (7-36)-Amide *<br />
BD <strong>P700</strong> versus EDTA <strong>Plasma</strong><br />
BD <strong>P700</strong> K 2 EDTA<br />
Mean 2.5 1.1<br />
Standard Deviation 3.4 1.8<br />
Minimum 0 0<br />
Maximum 10.2 4.3<br />
Total Subjects 12 12<br />
BD <strong>P700</strong>: Standard Electrolyte<br />
and Chemistry 8 Profile Analysis *<br />
Sodium<br />
(nmol/L)<br />
Chloride<br />
(nmol/L)<br />
BD <strong>P700</strong> versus EDTA <strong>Plasma</strong><br />
HCO3 (nmol/L)<br />
BUN<br />
(mg/dL)<br />
Creatinine<br />
(mg/dL)<br />
Glucose<br />
(mg/dL)<br />
Total Protein<br />
(g/dL)<br />
Albumin<br />
(g/dL)<br />
Table 2. Comparison of plasma electrolyte and Chemistry 8 profile <strong>for</strong> whole blood<br />
samples collected into BD <strong>P700</strong> and K 2 EDTA Tubes. <strong>Blood</strong> was drawn directly<br />
from 12 subjects into BD <strong>P700</strong> and K 2 EDTA evacuated tubes at room temperature.<br />
An aliquot of each plasma sample was prepared <strong>for</strong> use in Standard Electrolyte and Chemistry<br />
8 Profile analyses. Comparable quantitation of analytes were observed using BD <strong>P700</strong><br />
and K 2 EDTA Tubes.<br />
* Based on studies using 12 samples; cannot be guaranteed to be statistically valid.<br />
Bilirubin<br />
(mg/dL)<br />
ALT<br />
(U/L)<br />
AST<br />
(U/L)<br />
147.3 99.5 16.5 15.2 0.9 101.5 7.5 4.6 0.5 21.2 25.8<br />
SD 3.8 2.2 2.1 3.4 0.2 18.1 0.4 0.3 0.5 7.4 4.9<br />
K 2 EDTA 140.5 97.3 17.9 15.2 0.8 101.2 7.5 4.5 0.5 23.4 23.8<br />
SD 2.2 2.6 2.0 3.1 0.2 19.1 0.3 0.3 0.5 11.1 5.5
Reference<br />
Number<br />
Kit Contents<br />
20<br />
BD <strong>P700</strong> Product Description<br />
366473<br />
BD <strong>P700</strong> <strong>Blood</strong> <strong>Collection</strong> Tubes<br />
Ten (10) tubes/foil pouch, Two (2) foil pouches/box with desiccant<br />
1 Product Instructions<br />
1 “Research Use Only” Certification Card<br />
Required Accessories **<br />
Description Reference Number<br />
BD Vacutainer ® Safety-Lok <strong>Blood</strong> <strong>Collection</strong> Set<br />
(21 G butterfly needle, 12" (304.8 mm) tubing:<br />
sterile, use once and discard)<br />
BD Vacutainer ® One Use Holder 364815<br />
Region Ordering Technical<br />
US<br />
† BD <strong>P700</strong> is sold directly by BD. Not available through distributors.<br />
** Product must be used with a 12" blood collection set, such as BD Vacutainer ® Safety-Lok <strong>Blood</strong> <strong>Collection</strong> Set.<br />
BD, BD Logo and all other trademarks are property of<br />
Becton, Dickinson and Company. © 2008 BD 06/08 VS7641-2<br />
367281 (North America)<br />
367286 (Outside North America)<br />
Ordering and Technical In<strong>for</strong>mation †<br />
888.237.2762 (phone orders)<br />
800.847.2220 (fax orders)<br />
800.631.0174 (phone)<br />
vacutainer_techservices@bd.com<br />
(email)<br />
Canada 800.268.5430 ext. 5567 800.268.5430 ext. 5567<br />
All other areas Please visit www.bd.com to locate your regional office<br />
1 Becton Drive<br />
Franklin Lakes, NJ 07417<br />
www.bd.com/proteomics