New Researches in Biotechnology - Facultatea de Biotehnologii ...

Proceeding of the 4 rd International Symposium“NEW RESEARCH IN BIOTECHNOLOGY” USAMV Bucharest, Romania, 2011azapylones was obtained after 106 h, (figure 5) with a OD=3.8 units units, whilst maximumconcentration of orange compounds was obtained after 105 h, when a OD = 4.2 wasobtained (figure 6.)Table 1. Culture media used o obtain Monascus metabolite in submerged mediaCulture media with starchCulture media with ethanolStarch, g/L 30 Glutamic acid, g/L 5Peptone, g/L 4 K 2 HPO 4, g/L 5glutamic acid, g/L 1 KH 2 PO 4, g/L 5bidistiled water up to1L MgSO 4 •7H 2 O, g/L 0.5Culture media with dextrose CaCl 2, g/L 0.5Dextrose, g/L 30 FeSO 4 •7H 2 O), g/L 0.5NaNO 3, g/L 0.1 ZnSO 4 •7H 2 O, g/L 0.01MgSO 4, g/L 0.05 MnSO 4 •H 2 O, g/L 0.03FeSO 4 , g/L 0.01 Ethanol, g/L 20ZnSO 4 , g/L 0.08 bidistiled water up to1LMnSO 4 , g/L 0.03bidistiled waterup to1LMaximum concentration for yellow azaphylones was attain in 114 h with a OD =2.85 (figure 8.)In media with etanol the behaviour of Monascus sp 1 is the same like in the caseof starch media (without pigment production). In the case of strain Monascus sp.2 ,azaphylone appear, but in the concentration less than that obtained in starch culture media.So, in this case the productions of azaphylones red and yelow attain the maximumconcentration after 145h, corresponding to an OD=1(figure 8 and figure 10), whereas thecontent of orange azaphylone attain a maximum concentration after 135 h with an OD =0.27 (figure 9.In submerged media which contain dextrose, the both strains produce azaphylones,but content of their is less than azaphylone concentration obtained in starch or etanolculture media. The Monascus sp. 1 strain give a maximum concentration of red azaphilonesafter 160 h, when obtain an OD =0.75 (figure 11). After 164 h, a maximum concentrationof orange pigment is obtained (figure 12) with an OD =1. Two hour later (at 166 h) amaximum concentration of yellow pigment was obtained; with an OD =1 (figure 13),Monascus sp. 2 give a maximum concentration of red azaphylones after 153 h,with an OD =0.75 (figure 14). After 166 h of biosynthesis, a maximum concentration oforange pigment was obtained (figure 15) with an OD=1. After that, at 177h a maximumconcentration of yellow pigment was obtained, with an OD =1.2 (figure 16)Fungal mycotoxin formation in submerged media. Fungi from Monascus genusproduce one mycotoxin: citrinin. Content of this toxic compound in food, raw materials forfood industry or fodder depend of Monascus strains and culture media.85