New Researches in Biotechnology - Facultatea de Biotehnologii ...

New Researches in Biotechnology - Facultatea de Biotehnologii ... New Researches in Biotechnology - Facultatea de Biotehnologii ...

10.07.2015 Views

Proceeding of the 4 rd International Symposium“NEW RESEARCH IN BIOTECHNOLOGY” USAMV Bucharest, Romania, 2011SECTION III: FOOD BIOTECHNOLOGYEVALUATION OF INULIN GELLING PROPERTIES IN NEW TYPES OFJELLIESMAIJA KRONBERGA, DAINA KARKLINA, DACE KLAVA, RUTA GALOBURDAFaculty of Food Technology, Latvia University of Agriculture, Liela iela 2, Jelgava, Latvia, LV 3001Tel/fax No 37163022829, e-mail:maija.kronberga@inbox.lvAbstract. Inulin-type fructans have become a topic of much interest for both the food industry and forresearchers (Roberfroid, 2005). At high concentrations in water, above 25% for standard chicoryinulin and above 15% for long – chain material, inulin has gelling properties (Glibowski, 2011).Therefore the objective of this research work was to evaluate effect of agar-agar and inulin on thestructural properties of experimental gels.The research was carried out at the Latvia University of Agriculture, Faculty of Food Technology. Ingel production the inulin powder was used to make mixed gels from the agar-agar and inulin. Toprepare control samples the following recipe was used: agar-agar (2 g), sucrose (28 g), water(145 g), citric acid (1 g) and inulin powder (24 g). In the research amount of agar-agar was varied inthe following range: 1.0; 1.5; 2.0; 2.5 g. Agar-agar water solutions were boiled for 5 minutes at thetemperature of 100 ºC, then sucrose and citric acid were added to the solution and cooled down to85 ºC. Then inulin powder was added, mixture stirred and kept for 5 minutes at the temperature85 ºC. The solution was hot filled in polystyrene containers (150 ml), which were sealed with lids andcooled, down to 18 ºC.The gel strength (hardness) was characterised by cutting force. Texture was determined using aTexture Analyser TA.XT Plus. Wire cutter (A/BC) was used to slice the jelly samples. Measurementswere carried out on samples at room temperature on the next day after gel samples were prepared.Structure of agar-agar and inulin powder dissolved in water, control samples with agar-agar, andinulin, as well as gel samples with both polysaccaharides were analysed under the triocularmicroscope Axioskop 40. Pictures were taken by digital compact camera Canon PowerShot A620 via16 × 10 or 16 × 40 magnification of the microscopeIn experimental gel samples, inulin concentration influenced the strength of the gels. The decrease inhardness of the gels can be explained by different properties of inulin and agar-agar. The strength ofthe gels became weaker when inulin was used as agar-agar substitute.Keywords: inulin powder, agar-agar, gels1. INTRODUCTIONJellies are not basic foods, but they are good complements to a diet if they are eaten incorrect amounts (Figuerola et al., 2007). Jellies are high-energy products, meaning that theproducts are not suitable or advisable for people who have glycemic problems, obesity,diabetes, and cardiovascular diseases.70

Proceeding of the 4 rd International Symposium“NEW RESEARCH IN BIOTECHNOLOGY” USAMV Bucharest, Romania, 2011Sugar in jellies is essential to form not only to product sweet taste, but also the productstructure and gives to product calories (Bayarri et al., 2004). Some polysaccarides can beused to replace sugar in different sweets obtaining products with lower calories.Inulin is legally classified as food or a food ingredient, and not as additive in all countrieswhere it is processed in a number of different ways for the health food market (Roberfroid,2005, Frack, 2002; Kaur et al., 2002). Inulin has been defined as a polydispersecarbohydrate consisting mainly of β – (2→1) fructosylfructose links (Roberfroid, 2005).The most common are: native inulin with an average degree of polymerization (DP) of 10–12, inulin containing short chain fractions (with DP from 2 to 10) and a high performanceinulin with an average DP no less than 23 (Roberfroid, 2005).Inulin offers technological properties for a wide scope of food applications as well asimportant nutritional benefits. At high concentrations (> 25% in water for native and > 15%for long-chain inulin) inulin has gelling properties and forms a participle gel networkingafter shearing (Kim et al. 2001, Glibowski and Wasko 2008, Glibowski, 2009; Glibowskiand Bukowska, 2011). When inulin is thoroughly mixed with water, a white creamystructure is obtained that can easily be incorporated in foods to replace fat. One of is its fatmimetic property which is used in the production of yogurts with decreased caloricity andfull fat taste (Guven et al., 2005) and in ice-cream (El-Nagar et al., 2002) production.Inulin incorporation into baked goods allows the replacement of sugar, fibre enrichmentand better moisture retention properties (Frack, 2002). Inulin has found an interestingapplication as low calorie bulk ingredient in chocolate to replace sugar. Inulin syrup alsocan be used to substitute sugar in low-sugar jelly formulations by obtaining differentproduct quality (Kronberga et al., 2011; Kronberga and Karklina, 2011).Agar has been used for many centuries as a high performance gelling agent (Armisen et al.,2000; Stanley, 2006). Agar forms one of the strongest gels. Its ability to produce clear,colourless, odourless, and natural gels without the support of other colloids has long beenexploited by the food industry not only as a stabilizer and gelling agent but also in themanufacturing of confectionery (Barrangou et al., 2006).Interactions between hydrocolloids may modify the gel characteristics. The polysaccharidediffers from other hydrocolloids in property of its high gel strength. The synergistic effectof inulin with other gelling agents constitutes additional advantage in all these applications.In several products inulin, and especially long chain version, can even (partially) replacegelatine, starch, maltodextrin, and other stabilizers. As of present jelly structure propertiesaffected by agar-agar and inulin have not been investigated decreasing the content of agarand increasing the content of inulin in jellies. The knowledge of the mixed gels as well asthe stability of such gels becomes very important for the development new products.Therefore the objective of this research work was to evaluate effect of agar-agar and inulinon the structural properties of experimental gels.2. MATERIALS AND METHODSInulin granulated powder made from chicory root (Orafti GR produced by BENEO-Orafti,Belgium) was used in the experiments. Agar powder produced by AgarNordS, Estonia (E406) was used for preparation of experimental jellies. Sugar (Dansukker, Denmark) andcitric acid (E 330) were purchased in local retailing and added to experimental gels.71

Proceed<strong>in</strong>g of the 4 rd International Symposium“NEW RESEARCH IN BIOTECHNOLOGY” USAMV Bucharest, Romania, 2011Sugar <strong>in</strong> jellies is essential to form not only to product sweet taste, but also the productstructure and gives to product calories (Bayarri et al., 2004). Some polysaccari<strong>de</strong>s can beused to replace sugar <strong>in</strong> different sweets obta<strong>in</strong><strong>in</strong>g products with lower calories.Inul<strong>in</strong> is legally classified as food or a food <strong>in</strong>gredient, and not as additive <strong>in</strong> all countrieswhere it is processed <strong>in</strong> a number of different ways for the health food market (Roberfroid,2005, Frack, 2002; Kaur et al., 2002). Inul<strong>in</strong> has been <strong>de</strong>f<strong>in</strong>ed as a polydispersecarbohydrate consist<strong>in</strong>g ma<strong>in</strong>ly of β – (2→1) fructosylfructose l<strong>in</strong>ks (Roberfroid, 2005).The most common are: native <strong>in</strong>ul<strong>in</strong> with an average <strong>de</strong>gree of polymerization (DP) of 10–12, <strong>in</strong>ul<strong>in</strong> conta<strong>in</strong><strong>in</strong>g short cha<strong>in</strong> fractions (with DP from 2 to 10) and a high performance<strong>in</strong>ul<strong>in</strong> with an average DP no less than 23 (Roberfroid, 2005).Inul<strong>in</strong> offers technological properties for a wi<strong>de</strong> scope of food applications as well asimportant nutritional benefits. At high concentrations (> 25% <strong>in</strong> water for native and > 15%for long-cha<strong>in</strong> <strong>in</strong>ul<strong>in</strong>) <strong>in</strong>ul<strong>in</strong> has gell<strong>in</strong>g properties and forms a participle gel network<strong>in</strong>gafter shear<strong>in</strong>g (Kim et al. 2001, Glibowski and Wasko 2008, Glibowski, 2009; Glibowskiand Bukowska, 2011). When <strong>in</strong>ul<strong>in</strong> is thoroughly mixed with water, a white creamystructure is obta<strong>in</strong>ed that can easily be <strong>in</strong>corporated <strong>in</strong> foods to replace fat. One of is its fatmimetic property which is used <strong>in</strong> the production of yogurts with <strong>de</strong>creased caloricity andfull fat taste (Guven et al., 2005) and <strong>in</strong> ice-cream (El-Nagar et al., 2002) production.Inul<strong>in</strong> <strong>in</strong>corporation <strong>in</strong>to baked goods allows the replacement of sugar, fibre enrichmentand better moisture retention properties (Frack, 2002). Inul<strong>in</strong> has found an <strong>in</strong>terest<strong>in</strong>gapplication as low calorie bulk <strong>in</strong>gredient <strong>in</strong> chocolate to replace sugar. Inul<strong>in</strong> syrup alsocan be used to substitute sugar <strong>in</strong> low-sugar jelly formulations by obta<strong>in</strong><strong>in</strong>g differentproduct quality (Kronberga et al., 2011; Kronberga and Karkl<strong>in</strong>a, 2011).Agar has been used for many centuries as a high performance gell<strong>in</strong>g agent (Armisen et al.,2000; Stanley, 2006). Agar forms one of the strongest gels. Its ability to produce clear,colourless, odourless, and natural gels without the support of other colloids has long beenexploited by the food <strong>in</strong>dustry not only as a stabilizer and gell<strong>in</strong>g agent but also <strong>in</strong> themanufactur<strong>in</strong>g of confectionery (Barrangou et al., 2006).Interactions between hydrocolloids may modify the gel characteristics. The polysacchari<strong>de</strong>differs from other hydrocolloids <strong>in</strong> property of its high gel strength. The synergistic effectof <strong>in</strong>ul<strong>in</strong> with other gell<strong>in</strong>g agents constitutes additional advantage <strong>in</strong> all these applications.In several products <strong>in</strong>ul<strong>in</strong>, and especially long cha<strong>in</strong> version, can even (partially) replacegelat<strong>in</strong>e, starch, malto<strong>de</strong>xtr<strong>in</strong>, and other stabilizers. As of present jelly structure propertiesaffected by agar-agar and <strong>in</strong>ul<strong>in</strong> have not been <strong>in</strong>vestigated <strong>de</strong>creas<strong>in</strong>g the content of agarand <strong>in</strong>creas<strong>in</strong>g the content of <strong>in</strong>ul<strong>in</strong> <strong>in</strong> jellies. The knowledge of the mixed gels as well asthe stability of such gels becomes very important for the <strong>de</strong>velopment new products.Therefore the objective of this research work was to evaluate effect of agar-agar and <strong>in</strong>ul<strong>in</strong>on the structural properties of experimental gels.2. MATERIALS AND METHODSInul<strong>in</strong> granulated pow<strong>de</strong>r ma<strong>de</strong> from chicory root (Orafti GR produced by BENEO-Orafti,Belgium) was used <strong>in</strong> the experiments. Agar pow<strong>de</strong>r produced by AgarNordS, Estonia (E406) was used for preparation of experimental jellies. Sugar (Dansukker, Denmark) andcitric acid (E 330) were purchased <strong>in</strong> local retail<strong>in</strong>g and ad<strong>de</strong>d to experimental gels.71

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