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Proceeding of the 4 rd International Symposium“NEW RESEARCH IN BIOTECHNOLOGY” USAMV Bucharest, Romania, 201110 000 2 0 0Cabernet Sauvignon /1000 8 0 0GLRaV-3 10 000 22 1 4.5Grasă de Cotnari /1000 15 0 0GFkV 10 000 9 9 1001000 7 6- ArMV-free 0Unidentified genotype (1)/0 -GFkV-freeArMV+GFkV 10 000 9 8– ArMv-free 00- GFkV-freeUnidentified genotype (2)/ 1000 - - -GFLV+GLRaV-1 10 000 7 0 0The succes of electrotherapy in producing virus- free plants depends upon bothplant regeneration and virus elimination rates (M.H. HORMOZI-NEJAD [22].Taking into consideration that both, antiviral treatment with electric current and invitro culture, can be considered stress factors responsible for inducing genetic variation, itis compulsory to check the obtained plants for genetic stability, fidelity and uniformity.Regenerated grapevine after exposure to electrotherapy in continous electric field weremorphologically identical to the control (non-treated plants), as shown in the studiescomparing their random amplified polymorphic DNA (RAPD) profiles (I.C. GUȚĂ & al.[14]).48CONCLUSIONSThe influence of alternating electric current and the behaviour of grapevinegenotypes in the presence of virus infection, during in vitro axillary buds proliferation,could not be correlated.The behaviour of viruses in sanitation process is different in simple or mixedinfections; electrotherapy in alternating current of single infected microshoots producedGLRaV-1-, GLRaV-3- or GFkV-free grapevine in different percentage, but no viruselimination in mixed infections containing one of these viruses has been obtained.No satisfactory results have been achieved for GFLV eradication both in simpleand mixed infections.In order to increase the number of grapevine cultivars and clones needed to beavailable as healthy material, the certification program and, also, the virus-eliminationmethod for obtaining virus-free grapevines must be constantly developed.REFERENCES
Proceeding of the 4 rd International Symposium“NEW RESEARCH IN BIOTECHNOLOGY” USAMV Bucharest, Romania, 20111. Maninni, F. (2003): Virus elimination in grapevine and crop performance. Extended abstracts 14 thICVG Meeting, 12-17 September 2003, Locorotondo (Bari), Italy, 234-239.2. Tsvetkov, I., Atanassov, A., Jankulova, M., Vulchev,V., Todorov, I., Pandeliev, S., Katerov, K.,Atanassov, I., Hvarleva, T., Tsvetkov, Y., Genov, I., Dzambazova, T., Antonov, I., Marinov, L.,Dimitrov, E., Panamska, M., Varadinova, G., Slavova, K., Atanassov, I., Shishmanov, G., Ivanova,N., Laginova, M. (2005): Grapevine plant genetic resources improvement, preservation andbiodiversity. Biotechnol. & Biotechnol. Eq. Vol.19: 13-21.3. Grammatikaki, G. and Avgelis, A. (2009): Virus elimination in infected clones of four Greekgrapevine wine cultivars (Roditis, Xinomavro, Baftra and Savatiano) using in vitro thermotherapy andtip meristem culture. Extended abstracts 16 th ICVG Meeting, 31 Aug.- 4 Sept. 2009, Dijon, France,241-242.4. Křižan, B., Ondrušiková, E., Holleinová, V., Moravcová, K., Bláhová, L. (2009): Elimination ofGrapevine fanleaf virus in grapevine by in vivo and in vitro thermotherapy. Hort. Sci. (Prague). Vol.36, 3:105–108.5. Panattoni, A., D’Anna, F., Triolo, E. (2006): Improvement in grapevine chemotherapy. Extendedabstracts 15 th ICVG Meeting, 3-7 April 2006, Stellenbosch, South Africa, 139-141.6. Guţă, I.C., Buciumeanu, E.C., Vişoiu, E., Teodorescu, Al., Liţă, I. (2008): The effect of electricfield on in vitro regenerative processes and grapevine virus elimination, Simpozion aniversar: A״ 60-aaniversare a Facultăţii de ‏,״Horticultură A״ 10-a aniversare a specializării de ‏,״peisagistică 23 mai2008, Bucureşti, Lucrări ştiinţifice, UŞAMV, Seria B – LI -2008- Horticultură, 481-486.7. Bayati, SH., Shams-Bakhsh, M., Moieni, A. (2011): Elimination of grapevine virus A (GVA) bycryotherapy and electrotherapy. J. Agr. Sci. Tech. Vol. 13: 443-450.8. Gambino, G., Vallania, R., Gribaudo, I. (2009): Detection and elimination of grapevine fanleafvirus in callus, somatic embryos and regenerated plantlets of grapevine. Extended abstracts 16 th ICVGMeeting, 31 Aug. - 4 Sept. 2009, Dijon, France, 239-240.9. Gonzáles, J.E., Sánchez, R., Sánchez, A. (2006): Biophysical analysis of electric current mediatednucleoprotein inactivation process. Centro Agrícola Vol. 2: 42-47.10. Lozoya-Saldaña, H.F., Abelló, J., García G. (1996): Electrotherapy and shoot-tip cultureeliminate potato virus X in potatoes. Am. J. Potato Res. Vol. 73: 149-154.11. Igarza Castro, J., Hernández, P.R., Cruz Castelanos, B. (2001): Electrotherapy as an alternativefor elimination the DMV virus in tania. Manejo Integrado de Plagas Vol. 60: 57-60.12. Hernández, P.R., Bertrand, H., Lepoivre, P., Gonzáles, J.E., Rojas, X., Pairol, A., Gonzáles, Y.,Gonzáles, G.Y., Cortés, C. (2002): Diagnostico y saniaminieto de Banana Streak Virus (BSV) enMusa spp. Centro Agrícola 2: 42-47.13. Burger, J.G. (1989): Electrotherapy: a possible method to eliminate grapevine fanleaf virus fromgrapevine. Extended abstracts 9 th ICVG Meeting, Kiryat Anavim, Israel, 1987, 153.14. Guță, I.C., Buciumeanu, E.C., Gheorghe, R.N., Teodorescu, Al. (2010): Solutions to eliminategrapevine leafroll associated virus serotype 1+3 from V. vinifera L. cv. Ranâi Magaraci. Rom.Biotechnol. Lett. Vol.15, 1: 72–78.15. Murashige, T. and Skoog, F. (1962): A revised medium for rapid grow and bioassays withTabacco tissue culture. Physiol. Plant. 15: 473-49.49
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