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Proceeding of the 4 rd International Symposium“NEW RESEARCH IN BIOTECHNOLOGY” USAMV Bucharest, Romania, 2011LocalityNTG YM DON ZEA Impurities Moldy(ufc/g) (ufc/g) (g/kg) (g/kg) (%) grains (%)Rosiori de Vede 1.10·10 5 3.60·10 3 n.d.* n.d.* 4.87 0.05Videle 7.00·10 5 3.00·10 3 n.d.* n.d.* 7.7 0* not detectedIn Figure 1 is presented the evolution of DON content in all the wheat samples. Asit can be noticed, the highest value for DON is registered in Dâmboviţa (686.51 g/kg),followed by Dolj (554.39 g/kg) and Călăraşi (397.8 g/kg).In all wheat samples studied, the mycotoxin content was below the maximumlevels allowed for unprocessed cereals established by CE no. 1881/2006 regulation; exceptfor Teleorman county where samples from Bogdana and Mosteni locality exceed the limitfor ZEA content (100 g/kg), namely 112.657 g/kg, respectively 191.052 g/kg (Fig.2).800700600DON, µg/kg5004003002001000Dolj Călăraşi Olt Dâmboviţa Buzău Brăila Mehedinţi TeleormanFig.1. Evolution of DON content in wheat samples from the counties analyzed.There were samples in which mycotoxin content was not detected: 92.7% ofsamples analyzed for ZEA, respectively 34.8% for DON content, the values being belowthe limit of detection of the method used for analysis. So, DON presence is higher thanZEA in wheat samples studied.In 5 counties, namely, Dolj, Olt, Dâmboviţa, Buzău and Mehedinţi, ZEA contentwas not detected in all the wheat samples studied. The only values detected for ZEAcontent in some localities from Călăraşi, Brăila and Teleorman are presented in Fig.2.Also, in all the samples from Călăraşi, Olt and Dâmboviţa, DON content wasdetected.In Fig.3 is shown a comparison between the highest values for DON found in awheat sample from each of the county analyzed and the corresponding value for ZEA forthe same sample.194
Proceeding of the 4 rd International Symposium“NEW RESEARCH IN BIOTECHNOLOGY” USAMV Bucharest, Romania, 2011ZEA, µg/kg200180160140120100806040200Călăraşi Brăila TeleormanFig.2. ZEA content in wheat samples analyzed.Mycotoxin, µg/kg7006005004003002001000Dolj Olt Buzău MehedinţiDON ZEAFig.3. Comparison between DON and ZEA content in the same locality.4. CONCLUSIONSIn this study, for all wheat samples (Triticum aestivum, Dropia variety) studiedfrom 8 different regions situated in southern Romania, the mycotoxin content was belowthe maximum levels allowed for unprocessed cereals established by European Regulation,except for Teleorman county were 2 samples exceed the limit for ZEA content.In the same county, Dropia variety had different mycotoxin loads, varying frombelow the limit of detection, of the method used for analysis, to higher values.195
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