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192Proceeding of the 4 rd International Symposium“NEW RESEARCH IN BIOTECHNOLOGY” USAMV Bucharest, Romania, 20113. RESULTS AND DISCUSSIONSThe microbiological analysis: total number of germ (NTG), yeasts and molds(YM), mycotoxin content (DON, ZEA) and percentage of impurities and foreign bodies andmoldy grains are presented in Table 1 for the wheat samples analyzed in all the 8 counties.For 33.3% of the samples analyzed, impurities and foreign bodies content exceedsthe maximum accepted percentage of 15% due to the percentage of kernels affected bypests (bug especially) and sprouted or fungal contamination.In the same county, Dropia variety had different mycotoxin values, varying frombelow the limit of detection to higher values. For example, in Teleorman county DONcontent varied from not detected values to 279.94 g/kg. Also, same situation in Dolj, Buzăuand Brăila. This can be due to the local environmental condition (soil conditions,meteorological conditions, harvesting agricultural technologies).Table 1. Results obtained for wheat samples of 2010 crop for different counties.LocalityNTG(ufc/g)YM(ufc/g)DON(g/kg)ZEA(g/kg)Impurities(%)Moldygrains (%)Dolj countyMischii 1.20·10 6 1.70·10 4 11.87 n.d.* 6.33 0Teslu 4.80·10 5 2.00·10 4 31.01 n.d.* 4.53 0.1Scaesti 4.50·10 5 7.30·10 3 11.97 n.d.* 13.37 0Ciupercenii-Noi 3.20·10 5 1.00·10 3 12.96 n.d.* 4.08 0Macesu de Sus 8.20·10 4 4.00·10 3 43.94 n.d.* 1.24 0Daneti 2.00·10 6 4.50·10 4 554.39 n.d.* 32.42 0.33Plenita 1.50·10 5 1.00·10 4 n.d.* n.d.* 4.8 0Motatei 4.00·10 4 9.00·10 3 6.51 n.d.* 7.11 0.2Izvoare 9.00·10 4 4.00·10 3 10.14 n.d.* 5.01 0Rast 2.60·10 5 6.40·10 3 6.41 n.d.* 4.73 0.35Dragotesti 2.50·10 5 2.00·10 4 12.02 n.d.* 4.9 0Leu 1.00·10 6 6.00·10 3 25.5 n.d.* 10.34 0.03Calafat 5.50·10 6 1.00·10 3 5.05 n.d.* 3.76 0Călăraşi countyBelciugatele 2.60·10 6 8.00·10 3 7.29 n.d.* 11.49 0.15Lupsanu 1.10·10 5 8.00·10 3 6.67 n.d.* 16.72 0.4Plataresti 1.70·10 5 1.20·10 4 298.72 n.d.* 4.39 0Dor Marunt 1.40·10 5 4.00·10 3 5.11 n.d.* 14.69 0.18St. Voda 6.40·10 5 2.10·10 4 9.67 n.d.* 38.52 0.15Al. Odobescu 2.70·10 5 1.90·10 4 6.44 n.d.* 20.85 0.13Mitreni 4.70·10 5 3.50·10 4 9.59 n.d.* 19.32 1.23Independentei 8.20·10 4 1.20·10 4 8.54 n.d.* 15.01 0.2Vlad Tepes 3.10·10 5 7.00·10 3 5.25 n.d.* 22.82 0.03Fundeni 4.10·10 5 8.00·10 3 397.8 0.3709 4.43 0Sohatu 7.00·10 4 4.00·10 3 125.84 n.d.* 1.71 0.02Galbinasi 1.90·10 5 1.80·10 4 147.56 n.d.* 15.19 0Olt countySlatina Z3 3.20·10 5 5.00·10 4 52.78 n.d.* 10.73 0.1
Proceeding of the 4 rd International Symposium“NEW RESEARCH IN BIOTECHNOLOGY” USAMV Bucharest, Romania, 2011LocalityNTG(ufc/g)YM(ufc/g)DON(g/kg)ZEA(g/kg)Impurities(%)Moldygrains (%)Studina Z9 1.10·10 6 1.40·10 4 0.09 n.d.* 18.74 0.48Corabia Z10 7.20·10 5 1.00·10 4 44.2 n.d.* 15.4 0.07Samburesti Z1 4.40·10 5 9.00·10 4 45.4 n.d.* 7.25 0Piatra Olt Z5 5.30·10 5 8.20·10 4 29.04 n.d.* 10.39 0Bals Z4 4.50·10 5 1.10·10 4 233.46 n.d.* 13.76 0Valea Mare Z6 1.90·10 5 1.00·10 3 15.79 n.d.* 6.05 0Ianca Z11 8.70·10 5 2.10·10 4 18.36 n.d.* 17.88 0.22Dâmboviţa countyDobra 1.60·10 5 7.00·10 3 27.01 n.d.* 6.71 0Contesti 8.00·10 4 1.00·10 2 686.51 n.d.* 4.99 0Selbru, Visina 4.30·10 5 1.20·10 4 58.55 n.d.* 8.89 0Dragodana, Matasaru 1.60·10 5 7.30·10 4 390.17 n.d.* 4.85 0Buzău countyPogoanele, Scutelnici 1.30·10 6 3.60·10 4 n.d.* n.d.* 10.26 0.25Vadu Pasii, Sageata 1.50·10 5 1.20·10 3 n.d.* n.d.* 7.46 0.2Padina, Luciu 2.50·10 5 9.40·10 3 n.d.* n.d.* 16.04 0.3Puiesti, Boldu 2.20·10 5 7.00·10 2 n.d.* n.d.* 9.42 0.32Susetu, C.A.Roseti 5.30·10 5 5.00·10 3 n.d.* n.d.* 12.02 0.15Mihailesti 1.40·10 5 1.10·10 4 n.d.* n.d.* 26.89 0.13Gl. Silistea 2.50·10 5 6.00·10 3 n.d.* n.d.* 9.32 0.08P. Calmau 1.90·10 5 7.40·10 3 17.98 n.d.* 16.36 0.32Puiesti, Boldu 5.10·10 5 5.50·10 3 31.28 n.d.* 9.81 0.02Brăila countyBrăila Z1 3.40·10 5 1.30·10 4 64.07 10.101 5.55 0.08Brăila Z2 3.10·10 6 6.70·10 4 n.d.* n.d.* 12.55 0.72Brăila Z3 1.00·10 5 1.60·10 3 n.d.* n.d.* 17.26 0.13Brăila Z4 3.50·10 5 3.90·10 3 94.96 0.63224 7.71 0.65Mehedinţi countyBurila Mare 1.30·10 5 1.70·10 3 n.d.* n.d.* 3.17 0.2Vanju Mare 3.10·10 6 2.50·10 3 n.d.* n.d.* 9.73 0.05Garla Mare 1.40·10 5 1.80·10 4 n.d.* n.d.* 6.46 0.07Teleorman countyNasturelu 1.00·10 5 3.30·10 3 n.d.* n.d.* 14.81 0Smardioasa 3.50·10 5 5.20·10 4 n.d.* n.d.* 28.94 0.23Turnu Magurele 1.40·10 7 4.00·10 3 n.d.* n.d.* 11.74 0.17Balaci 3.50·10 5 1.70·10 4 22.37 n.d.* 20.84 0Magura 4.10·10 5 7.00·10 3 74.59 n.d.* 15.25 0.1Zimnicea 2.40·10 6 7.90·10 5 n.d.* n.d.* 12.1 1.06Traian 2.20·10 6 1.10·10 3 n.d.* n.d.* 24.37 0Bogdana 4.60·10 5 1.70·10 3 185.1 112.657 24.48 0.08Botoroaga 1.60·10 5 5.20·10 3 17.5 n.d.* 15.05 0.28Ciolanesti 3.50·10 5 3.10·10 3 n.d.* n.d.* 7.39 0Calmatui 4.80·10 5 9.10·10 3 n.d.* n.d.* 27.17 0Piatra 1.10·10 5 2.00·10 3 n.d.* n.d.* 11.88 0.07Mosteni 7.50·10 5 5.20·10 3 279.94 191.052 26.5 0.05Vitanesti 1.50·10 5 1.40·10 5 n.d.* n.d.* 6.04 0193
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