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PDA-310nmEtalon 2 ppm300280260240220200180160140120100806040200Proceeding of the 4 rd International Symposium“NEW RESEARCH IN BIOTECHNOLOGY” USAMV Bucharest, Romania, 2011300280260NameRetention TimeArea240220200amAU18016014012010080resveratrol 9.428 880792mAU60402000 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15Minutes300280PDA-310nmM 319VNameRetention TimeArea300280260260bmAU240220200180160140resveratrol 9.583 1540691240220200180160140mAU1201201001008080606040402020000 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15Minutes300280260PDA-310nmM 327VNameRetention TimeArea300280260240240220220200200180180cmAU160140160140mAU120120100100806040200resveratrol 9.268 195448060402000 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15MinutesFigure 3. Chromatogram for: (a) a trans-resveratrol standard solution; (b) a red wine sample; (c) awhite wine sample180
Proceeding of the 4 rd International Symposium“NEW RESEARCH IN BIOTECHNOLOGY” USAMV Bucharest, Romania, 2011Table 1 Trans-resveratrol content of Romanian red wines grouped on varieties, geographical originand harvest yearVariety Region Harvest yearTrans-resveratrol(mg/L)Muntenia (Valea Calugareasca) 2008 4.363Feteasca NeagraOltenia 2008 1.501Muntenia (Valea Calugareasca) 2009 6.589Muntenia (Dealu Mare) 2009 0.256Oltenia (Olt) 2009 1.717Muntenia (Tohani - Dealu Mare) 2008 0.262Cabernet SauvignonMuntenia (Urlati) 2008 0.499Oltenia (Mehedinti -Vinju Mare) 2008 0.025Oltenia - (Mehedinti -Vinju Mare) 2009 0.149Muntenia (Tohani - Dealu Mare) 2008 3.307MeroltMuntenia (Tohani – Dealu Mare) 2008 2.771Oltenia - (Mehedinti -Vinju Mare) 2008 1.621Pinot NoireMuntenia (Valea Calugareasca) 2008 2.369Muntenia (Valea Calugareasca) 2009 1.198Table 2. Trans-resveratrol content of Romanian white wines grouped on varieties, geographicalorigin and harvest yearVariety Region Harvest year Transresveratrol(mg/L)Feteasca Regala Muntenia (Dealu Mare-Pietroasele) 2008 n.d.Muntenia (Dealu Mare-Pietroasele) 2009 0.132Tamaioasa Romaneasca Muntenia (Dealu Mare-Pietroasele) 2008 n.d.Sauvignon Blanc Muntenia (Tohani – Dealu Mare) 2008 0.074Muntenia (Tohani – Dealu Mare) 2009 0.138Feteasca Alba Muntenia (Tohani – Dealu Mare) 2008 n.d.Muntenia (Tohani – Dealu Mare) 2009 n.d.Riesling Italian Muntenia (Valea Calugareasca) 2008 n.d.Muntenia (Valea Calugareasca) 2009 0.141Muscat Ottonel Muntenia (Urlati) 2009 0.159n.d. not detected (detection limit = 0.008 mg/L)4. CONCLUSIONSThis work enabled the implementation of an easy and reliable methodology fortrans-resveratrol analysis in wine. The use of direct injection in HPLC with PDA detectionis a practical method of analysis used by many laboratories. Validation studies for themethod show a detection limit of 0.008 mg/L, quantification limit of 0.026 mg/L and aglobal uncertainty of 23.5%.181
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