New Researches in Biotechnology - Facultatea de Biotehnologii ...

Proceeding of the 4 rd International Symposium“NEW RESEARCH IN BIOTECHNOLOGY” USAMV Bucharest, Romania, 2011The establishing of the sterilization methods as well as their correct application arevery important for the slightest mistakes regarding the sterilization formula (not respectingthe time, temperature and pressure conditions) can have severe consequences upon thepreservation and the quality of the product. At high temperature (sterilization) the resinsthat compose the protection lacquers can decompose and result in components with hightoxic potential (bisphenol) and can migrate from the package to the food stuff. [3,4]The traditional method for fabricating food cans in the food can industry implies theusing of tin covered with protection lacquers. This trend is in a continuous change due tothe using reglementations (european and national) regarding the dangerous endocrineeffects of the bisphenol found in the protection lacquers (Moller şi alţii, 2004). The framereglemenation 1935/2004/CE, as well as the new CE directives regarding the materials thatcome in contact with the food stuff impose migration limits for the specific components.On national level we can mention HG nr. 1197/2002 for the approving of the Regulationsregarding the materials and objects that come in contact with the food stuff and theadditional things regarding the using of the epoxy derivates in materials and objects thatcome in contact with the food stuff. [5] Being known the effects of the componentsmigration from the package in the food stuff it’s necessary to determine the degree ofstability of the materials by global migration and specific migration testes for thecomponents.2. MATERIALS AND METHODSWe studied in practical conditions the package and content of the can in the case oftwo different food stuff in cans fabricated with different tins and lacquers all for to comparethe protection abilities of several lacquer systems and for extending the shelf life.We used for the experiments the following types of tin lacquered cans: welded cans(3 pieces) Ø 99x48; pressed cans (2 pieces) Ø 73x30. For sealing these cans we used : Ø99DV caps and Ø 73DV caps all lacquered in the corresponding lacquering systems.We used the following lacquers: WHITE PL- inner protection white lacquer ofepoxyphenolic type; HE GOLD – inner protection yellow lacquer epoxyphenolic type andPL GOLD – inner protection yellow lacquer of modified epoxyphenolic type.The lab analysis of the lacquers observed:- the global migration of the components in the food simulants A, B and D and theorganoleptic exam of the samples and liquids after extraction:- the specific components migration in the simulant A : the bisphenol A content(BPA) by two methods: UV-VIS spectrometry and gas chromatography coupled with massspectroscopy GC-MS;-the heavy metals migration from the package in the simulant B by atomicabsorption spectroscopy (AAS) with graphite oven.The checking of the quality of the cans was led on a relevant lot taken from a meatproducts manufacturer production (pork meat in natural juice and fois gras).The filling and sterilization of the cans was led according to the specificity of theproduct. The can taken from the manufacturer according to STAS 3730-92 were stored inthe conditions specified for the product. The canned products were regularly analyzed from167