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Proceeding of the 4 rd International Symposium“NEW RESEARCH IN BIOTECHNOLOGY” USAMV Bucharest, Romania, 2011(Figure 6 and 7), during the observed period for both “Endivia mix“lettuce (type A) andgreen lettuce type B).ABFigure 2 Mesophilic bacteria cell concentration during storage time for both types of lettuces(Endivia mix – A and green lettuce – B)ABFigure 3 Coliforms bacteria variation during storage time for both types of lettuces (Endivia mix – Aand green lettuce – B)AB160
Proceeding of the 4 rd International Symposium“NEW RESEARCH IN BIOTECHNOLOGY” USAMV Bucharest, Romania, 2011Figure 4 Yeasts and moulds variation during storage time for both types of lettuces(Endivia mix – A and green lettuce – B)During storage, there were high values for mesophilic bacteria cell concentrationfor type A lettuce, as an evidence of increased sensitivity of salad mix compared with greenlettuce, related to minimal processing (cutting, washing).Regarding BOPP film, using a perforated film (Ø 5mm), did not lead to favorableresults in terms of microbiological results.There was a microbiological degradation of green lettuce starting the day 4 ofstorage (higher values for yeasts and molds, total coliforms bacteria and mesophilicbacteria). The microorganisms growth was according with increased water activity, startingwith day 4 of storage. It must be considered the lower value of water vapor permeability ofBOPP film. Regarding Envidia mix lettuce, number of the coliforms bacteria, an indicatorfor safety of the product, is not considered representative for the comparative study of thethree materials. But after 7 ddays of storage, the lettuce packaged in LDPE film wasmaintained at the lowest value. We mention that the yeasts and molds value remained lowerthan 10 cfu/g for 7 days, for both nanocomposite-based LDPE films and increased only forLDPE film.In all cases no molds contamination was observed, but only with yeast.Sensory propertiesInitial properties of the lettuce:• Appearance: without signs of infection or foreign bodies;• Color: yellow-green to dark green;• Taste and smell: pleasant, slightly bitter, fresh lettuce, without strange taste or smell.Sensory modifications noted during the storage period are presented in Table 3and 4.Table 3 Sensory modifications of Endivia mix lettuceDate Sample Appearance Color Taste and smell ObservationsLDPE No signs of Specific - From yellowgreenPleasant, slightlyDay 1 D20A- Amp infestationto dark green bitter, specificD20A- C08Gfresh lettuceLDPE No signs of Specific - From yellowgreenPleasant, slightlyDay 3 D20A- Amp infestationto dark green bitter,specific161
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