New Researches in Biotechnology - Facultatea de Biotehnologii ...

Proceeding of the 4 rd International Symposium“NEW RESEARCH IN BIOTECHNOLOGY” USAMV Bucharest, Romania, 20113. RESULTS AND DISCUSSIONOptical spectroscopic characteristics of the pigment were investigated. The solubility of thepigment is better in ethanol than aqueous solution. For the pigment in ethanol, it can beseen in Figure 4, two absorption bands at 374 and 508 nm respectively. Using differentexcitation wavelengths, the fluorescence emission of the pigment was evidenced. Figure 5shows the fluorescence emission spectra of the Monascus metabolites in water (Figure 5A)and ethanol solution (Figure 5B). Three well structured emission bands were evidenced at430, 455 and 525 nm respectively in aqueous as well as in ethanol solution. Figure 6presents the fluorescence emission spectra of the Monascus metabolites in thechemiluminescent system luminol- hydrogen peroxide, in Tris-HCl pH 8.10, in directcomparison with the reference system and also with the Monascus metabolites in ethanolsolution. For the LH 2 /Tris-HCl, pH 8.10/ H 2 O 2 , the fluorescence emission at 424 nmcorresponds to LH 2 fluorescence emission. The red shifted emission of 10 nm (434 nm) inthe presence of the pigment was evidenced. In direct comparison with the free Monascusmetabolites in ethanol solution, it was observed that the fluorescence emission band at 525nm decreases. The feature may be due to the oxidation of the metabolites by hydrogenperoxide.By the chemiluminescence method, the antioxidant activity of the pigment wasestimated, (results presented in Table 1). The antioxidant activity found around 90% isattributed to a good stability to the oxidative processes. This behavior is also correlatedwith the increasing in the collagen concentration.Table 1. The chemiluminescence (CL) parameters in the LH 2 - H 2 O 2 system, in Tris-HCl buffer, pH8.10 (Reference system) (RS) in the presence of the pigment.System I CL , a.u. A, %RS 1799.05 -2% Monascus metabolites 496.9 72.384% Monascus metabolites 252.05 85.996% Monascus metabolites 185.05 89.718% Monascus metabolites 96.55 94.6315% Monascus metabolites 75.095 95.82Tests regarding cicatrisation effect. In the first step, the witness of conditioned reagent(respectively isopropyl myristate and water) was tested in order to establish his influenceregarding scars retraction. Result obtaining (data not shown) reveals no influence ofconditioning reagent. Regarding bioproduct derived from solid state (P), the average valueof surface lesion with the first day was 3,8 cm 2 , in the 3-days 2,84 cm 2 and in the days 14and 18, the average value was more accentuated for bioproduct which contain Monascussp.metabolite (P) in comparison with conditioning reagent (figure 7). These results indicatea possible cicatrisation effect of Monascus sp. metabolite obtained in solid statebiosynthesis.152